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Change in ammonia-oxidizing microorganisms in enriched nitrifying activated sludge

  • Environmental Biotechnology
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Abstract

In this study, sludge was taken from a municipal wastewater treatment plant that contained a nearly equal number of archaeal amoA genes (5.70 × 106 ± 3.30 × 105 copies mg sludge−1) to bacterial amoA genes (8.60 × 106 ± 7.64 × 105 copies mg sludge−1) and enriched in three continuous-flow reactors receiving an inorganic medium containing different ammonium concentrations: 2, 10, and 30 mM NH +4 –N (28, 140, and 420 mg N l−1). The abundance and communities of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in enriched nitrifying activated sludge (NAS) were monitored at days 60 and 360 of the operation. Early on, between day 0 and day 60 of reactor operation, comparative abundance of AOA amoA genes to AOB amoA genes varied among the reactors depending on the ammonium levels found in the reactors. As compared to the seed sludge, the number of AOA amoA genes was unchanged in the reactor with lower ammonium level (0.06 ± 0.04 mgN l−1), while in the reactors with higher ammonium levels (0.51 ± 0.33 and 0.25 ± 0.10 mgN l−1), the numbers of AOA amoA genes were deteriorated. By day 360, AOA disappeared from the ammonia-oxidizing consortiums in all reactors. The majority of the AOA sequences from all NASs at each sampling period fell into a single AOA cluster, however, suggesting that the ammonium did not affect the AOA communities under this operational condition. This result is contradictory to the case of AOB, where the communities varied significantly among the NASs. AOB with a high affinity for ammonia were present in the reactors with lower ammonium levels, whereas AOB with a low affinity to ammonia existed in the reactors with higher ammonium levels.

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Acknowledgements

This work was supported by the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund) and the Thailand Research Fund under grant no. MRG5280086. Additionally, we would like to express our appreciation to the Bangkok Metropolitan Administration (BMA) for providing the seed sludge from one of its municipal WWTP. We also thank Professor Chongrak Polprasert for his guidance and Ms. Akiko Uyeda for her assistance in editing, polishing, and correcting the English language of our manuscript.

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Authors and Affiliations

  1. International Postgraduate Programs in Environmental Management, Graduate School, Chulalongkorn University, Bangkok, Thailand

    Puntipar Sonthiphand

  2. National Center of Excellence for Environmental and Hazardous Waste Management, Chulalongkorn University, Bangkok, Thailand

    Puntipar Sonthiphand & Tawan Limpiyakorn

  3. Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok, 10330, Thailand

    Tawan Limpiyakorn

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  1. Puntipar Sonthiphand
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  2. Tawan Limpiyakorn
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Correspondence to Tawan Limpiyakorn.

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Sonthiphand, P., Limpiyakorn, T. Change in ammonia-oxidizing microorganisms in enriched nitrifying activated sludge. Appl Microbiol Biotechnol 89, 843–853 (2011). https://doi.org/10.1007/s00253-010-2902-y

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