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Synthesis of a mesoporous functional copolymer bead carrier and its properties for glucoamylase immobilization

  • Biotechnological Products and Process Engineering
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Abstract

A series of mesoporous and hydrophilic novel bead carriers containing epoxy groups were synthesized by modified inverse suspension polymerization. Glycidyl methacrylate and acryloyloxyethyl trimethyl ammonium chloride were used as the monomers, and divinyl benzene, allyl methacrylate, and ethylene glycol dimethacrylate as crosslinking agents, respectively. The resulting carriers were employed in the immobilization of glucoamylase (Glu) with covalent bond between epoxy groups and enzymes. The activity recovery of the three series of immobilized Glus could reach 76%, 79%, and 86%, respectively. The immobilized Glus exhibit excellent stability and reusability than that of the free ones.

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Acknowledgments

We thank the National Natural Science Foundation of China (No. 50703017) and the Gansu Province Natural Science Foundation (No. 0710RJZA032) for the financial support given to this work.

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Authors and Affiliations

  1. Institute of Material Science and Engineering, Lanzhou University, Lanzhou, 730000, China

    Yongxiao Bai

  2. Institute of Biochemical Engineering & Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China

    Yanfeng Li & Lin Lei

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  1. Yongxiao Bai
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  2. Yanfeng Li
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Correspondence to Yongxiao Bai.

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Bai, Y., Li, Y. & Lei, L. Synthesis of a mesoporous functional copolymer bead carrier and its properties for glucoamylase immobilization. Appl Microbiol Biotechnol 83, 457–464 (2009). https://doi.org/10.1007/s00253-009-1864-4

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  • DOI: https://doi.org/10.1007/s00253-009-1864-4

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