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Fucoidans and fucoidanases—focus on techniques for molecular structure elucidation and modification of marine polysaccharides

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Abstract

The research field of fucoidans (sulphated polysaccharides from algae) and fucoidanases was strongly developing in recent years. Several different fucoidans and a few fucoidan-degrading enzymes were isolated and characterised. A high potential is seen in the medical exploitation of the fucoidans and its degradation products. This review gives an overview about the research of the last 5 years concerning fucoidan characterisation and application as well as enzyme detection, characterisation and production.

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References

  • Aisa Y et al (2005) Fucoidan induces apoptosis of human HS-sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways. Am J Hematol 78:7–14

    Article  CAS  PubMed  Google Scholar 

  • Albano RM, Mourao PAS (1986) Isolation, fractionation, and preliminary characterization of a novel class of sulfated glycans from the tunic of Styela plicata (Chordata Tunicata). J Biol Chem 261:758–765

    CAS  PubMed  Google Scholar 

  • Albuquerque IRL, Queiroz KCS, Alves LG, Santos EA, Leite EL, Rocha HAO (2004) Heterofucans from Dictyota menstrualis have anticoagulant activity. Braz J Med Biol Res 37:167–171

    Article  CAS  PubMed  Google Scholar 

  • Alves A-P, Mulloy B, Diniz JA, Mourao PAS (1997) Sulfated polysaccharides from the egg jelly layer are species-specific inducers of acrosomal reaction in sperms of sea urchins. J Biol Chem 272:6965–6971

    Article  CAS  PubMed  Google Scholar 

  • Andrykovitch G, Marx I (1988) Isolation of a new polysaccharide-digesting bacterium from a salt marsh. Appl Environ Microbiol 54:1061–1062

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Angulo Y, Lomonte B (2003) Inhibitory effect of fucoidan on the activities of crotaline snake venom myotoxic phospholipases A2. Biochem Pharmacol 66:1993–2000

    Article  CAS  PubMed  Google Scholar 

  • Araki Y, Katoh T, Urabe M, Kishi Y, Ishizuka I, Fujiyama Y (2004) The analysis of pyridylamino-dextran sulfate oligomers by high-performance liquid chromatography and a novel detection system for sulfated polysaccharides. Oncol Rep 12:363–367

    CAS  PubMed  Google Scholar 

  • Baba M, Snoeck R, Pauwels R, De Clercq E (1988) Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus. Antimicrob Agents Chemother 32:1742–1745

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bakunina IY et al (2000) Screening of marine bacteria for fucoidanases. Microbiology (Moscow)(Translation of Mikrobiologiya) 69:303–308

    CAS  Google Scholar 

  • Bakunina IY et al (2002) Degradation of fucoidan by the marine proteobacterium Pseudoalteromonas citrea. Microbiology (Moscow, Russian Federation)(Translation of Mikrobiologiya) 71:41–47

    Article  CAS  Google Scholar 

  • Barbeyron T, L’Haridon S, Corre E, Kloareg B, Potin P (2001) Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of Cytophaga uliginosa (Zobell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 51:985–997

    Article  CAS  PubMed  Google Scholar 

  • Beress A et al (1993) A new procedure for the isolation of anti-HIV compounds (polysaccharides and polyphenols) from the marine alga Fucus vesiculosus. J Nat Products 56:478–488

    Article  CAS  Google Scholar 

  • Berteau O, Mulloy B (2003) Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide. Glycobiology 13:29R–40R

    Article  CAS  PubMed  Google Scholar 

  • Berteau O, McCort I, Goasdoue N, Tissot B, Daniel R (2002) Characterization of a new alpha-L-fucosidase isolated from the marine mollusk Pecten maximus that catalyzes the hydrolysis of alpha-L-fucose from algal fucoidan (Ascophyllum nodosum). Glycobiology 12:273–282

    Article  CAS  PubMed  Google Scholar 

  • Bilan MI et al (2005) Effect of enzyme preparation from the marine mollusk Littorina kurila on fucoidan from the brown alga Fucus distichus. Biochemistry (Moscow) 70:1321–1326

    Article  CAS  Google Scholar 

  • Bilan MI, Grachev AA, Ustuzhanina NE, Shashkov AS, Nifantiev NE, Usov AI (2002) Structure of a fucoidan from the brown seaweed Fucus evanescens C.Ag. Carbohydr Res 337:719–730

    Article  CAS  PubMed  Google Scholar 

  • Bilan MI, Grachev AA, Ustuzhanina NE, Shashkov AS, Nifantiev NE, Usov AI (2004) A highly regular fraction of a fucoidan from the brown seaweed Fucus distichus L. Carbohydr Res 339:511–517

    Article  CAS  PubMed  Google Scholar 

  • Bilan MI, Grachev AA, Shashkov AS, Nifantiev NE, Usov AI (2006) Structure of a fucoidan from the brown seaweed Fucus serratus L. Carbohydr Res 341:238–245

    Article  CAS  PubMed  Google Scholar 

  • Bilan MI, Zakharova AN, Grachev AA, Shashkov AS, Nifantiev NE, Usov AI (2007) Polysaccharides of algae: 60. Fucoidan from the pacific brown alga Analipus japonicus (Harv.) winne (Ectocarpales, Scytosiphonaceae). Russ J Bioorgan Chem 33:38–46

    Article  CAS  Google Scholar 

  • Black WAP (1954) Seasonal variation in the combined L-fucose content of the common British Laminariaceae and Fucaceae. J Sci Food Agric 5:445–448

    Article  CAS  Google Scholar 

  • Black WAP, Dewar ET, Woodward FN (1952) Manufacture of algal chemicals. IV. Laboratory-scale isolation of fucoidin from brown marine algae. J Sci Food Agric 3:122–129

    Article  CAS  Google Scholar 

  • Boisson-Vidal C et al (2000) Relationship between antithrombotic activities of fucans and their structure. Drug Dev Res 51:216–224

    Article  CAS  Google Scholar 

  • Burtseva YV, Kusaikin MI, Sova VV, Shevchenko NM, Skobun AS, Zvyagintseva TN (2000) Distribution of fucoidan hydrolases and some glycosidases among marine invertebrates. Biologiya Morya (Vladivostok, Russian Federation) 26:429–432

    CAS  Google Scholar 

  • Chevolot L et al (1999) Further data on the structure of brown seaweed fucans: relationships with anticoagulant activity. Carbohydr Res 319:154–165

    Article  CAS  PubMed  Google Scholar 

  • Chevolot L, Mulloy B, Ratiskol J, Foucault A, Colliec-Jouault S (2001) A disaccharide repeat unit is the major structure in fucoidans from two species of brown algae. Carbohydr Res 330:529–535

    Article  CAS  PubMed  Google Scholar 

  • Church FC, Meade JB, Treanor RE, Whinna HC (1989) Antithrombin activity of fucoidan. The interaction of fucoidan with heparin cofactor II, antithrombin III, and thrombin. J Biol Chem 264:3618–3623

    CAS  PubMed  Google Scholar 

  • Colin S et al (2006) Cloning and biochemical characterization of the fucanase FcnA: definition of a novel glycoside hydrolase family specific for sulfated fucans. Glycobiology 16:1021–1032

    Article  CAS  PubMed  Google Scholar 

  • Corzo J, Perez-Galdona R, Leon-Barrios M, Gutierrez-Navarro AM (1991) Alcian blue fixation allows silver staining of the isolated polysaccharide component of bacterial lipopolysaccharides in polyacrylamide gels. Electrophoresis 12:439–441

    Article  CAS  PubMed  Google Scholar 

  • Cumashi A et al (2007) A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobiology 17:541–552

    Article  CAS  PubMed  Google Scholar 

  • Daniel R, Berteau O, Jozefonvicz J, Goasdoue N (1999) Degradation of algal (Ascophyllum nodosum) fucoidan by an enzymatic activity contained in digestive glands of the marine mollusc Pecten maximus. Carbohydr Res 322:291–297

    Article  CAS  Google Scholar 

  • Daniel R, Berteau O, Chevolot L, Varenne A, Gareil P, Goasdoue N (2001) Regioselective desulfation of sulfated L-fucopyranoside by a new sulfoesterase from the marine mollusk Pecten maximus: application to the structural study of algal fucoidan (Ascophyllum nodosum). Eur J Biochem 268:5617–5626

    Article  CAS  PubMed  Google Scholar 

  • Daniel R, Chevolot L, Carrascal M, Tissot B, Mourao PAS, Abian J (2007) Electrospray ionization mass spectrometry of oligosaccharides derived from fucoidan of Ascophyllum nodosum. Carbohydr Res 342:826–834

    Article  CAS  PubMed  Google Scholar 

  • De Reviers B, Marbeau S, Kloareg B (1983) Attempt to interpret the structure of fucoidans in relation to their localization in the cell wall of Phaeophyceae. Cryptogam Algol 4:55–62

    Google Scholar 

  • Dela Cruz Thomas Edison E, Schulz Barbara E, Kubicek Christian P, Druzhinina Irina S (2006) Carbon source utilization by the marine Dendryphiella species D. arenaria and D. salina. FEMS Microbiol Ecol 58:343–353

    Article  CAS  PubMed  Google Scholar 

  • Descamps V et al (2006) Isolation and culture of a marine bacterium degrading the sulfated fucans from marine brown algae. Mar Biotechnol 8:27–39

    Article  CAS  Google Scholar 

  • Deux J-F et al (2002) Low molecular weight fucoidan prevents neointimal hyperplasia in rabbit iliac artery in-stent restenosis model. Arterioscler Thromb Vasc Biol 22:1604–1609

    Article  CAS  PubMed  Google Scholar 

  • Dische Z, Shettles LB (1948) A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination. J Biol Chem 175:595–603

    CAS  PubMed  Google Scholar 

  • Dobashi K, Nishino T, Fujihara M, Nagumo T (1989) Isolation and preliminary characterization of fucose-containing sulfated polysaccharides with blood-anticoagulant activity from the brown seaweed Hizikia fusiforme. Carbohydr Res 194:315–320

    Article  CAS  PubMed  Google Scholar 

  • Duarte MER, Cardoso MA, Noseda MD, Cerezo AS (2001) Structural studies on fucoidans from the brown seaweed Sargassum stenophyllum. Carbohydr Res 333:281–293

    Article  CAS  PubMed  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Ekborg NA, Gonzalez JM, Howard MB, Taylor LE, Hutcheson SW, Weiner RM (2005) Saccharophagus degradans gen. nov., sp. nov., a versatile marine degrader of complex polysaccharides. Int J Syst Evol Microbiol 55:1545–1549

    Article  CAS  PubMed  Google Scholar 

  • Farias WRL, Valente A-P, Pereira MS, Mourao PAS (2000) Structure and anticoagulant activity of sulfated galactans: isolation of a unique sulfated galactan from the red algae Botryocladia occidentalis and comparison of its anticoagulant action with that of sulfated galactans from invertebrates. J Biol Chem 275:29299–29307

    Article  CAS  PubMed  Google Scholar 

  • Fitton JH, Irhimeh M, Falk N (2007) Macroalgal fucoidan extracts: a new opportunity for marine cosmetics. Cosmet Toilet 122:55–56, 58, 60–62, 64

    CAS  Google Scholar 

  • Fujimura T et al (2000) Fucoidan is the active component of Fucus vesiculosus that promotes contraction of fibroblast-populated collagen gels. Biol Pharm Bull 23:1180–1184

    Article  CAS  PubMed  Google Scholar 

  • Furukawa S, Fujikawa T, Koga D, Ide A (1992a) Purification and some properties of exo-type fucoidanases from Vibrio sp. N-5. Biosci Biotechnol Biochem 56:1829–1834

    Article  CAS  Google Scholar 

  • Furukawa S-I, Fujikawa T, Koga D, Ide A (1992b) Production of fucoidan-degrading enzymes, fucoidanase, and fucoidan sulfatase by Vibrio sp. N-5. Nippon Suisan Gakkaishi 58:1499–1503

    Article  CAS  Google Scholar 

  • Gerbst AG et al (2004) The synthesis and NMR and conformational studies of fucoidan fragments: VI. Fragments with an a-(1–2)-linked fucobioside unit. Russ J Bioorgan Chem (Translation of Bioorganicheskaya Khimiya) 30:137–148

    Article  CAS  Google Scholar 

  • Grauffel V, Kloareg B, Mabeau S, Durand P, Jozefonvicz J (1989) New natural polysaccharides with potent antithrombic activity: fucans from brown algae. Biomaterials 10:363–368

    Article  CAS  PubMed  Google Scholar 

  • Honya M, Mori H, Anzai M, Araki Y, Nisizawa K (1999) Monthly changes in the content of fucans, their constituent sugars and sulphate in cultured Laminaria japonica. Hydrobiologia 398/399:411–416

    Article  CAS  Google Scholar 

  • Ivanova EP et al (2002) Two species of culturable bacteria associated with degradation of brown algae Fucus evanescens. Microb Ecol 43:242–249

    Article  CAS  PubMed  Google Scholar 

  • Jhamandas Jack H, Wie Myung B, Harris K, MacTavish D, Kar S (2005) Fucoidan inhibits cellular and neurotoxic effects of beta-amyloid (A beta) in rat cholinergic basal forebrain neurons. Eur J Neurosci 21:2649–2659

    Article  CAS  PubMed  Google Scholar 

  • Karlsson A, Singh SK (1999) Acid hydrolysis of sulfated polysaccharides. Desulfation and the effect on molecular mass. Carbohydr Polym 38:7–15

    Article  CAS  Google Scholar 

  • Kelly S, Holtkamp A, Poth S, Lang S, Ulber R (2008) Untersuchungen zur potenziellen Fucoidanase-Aktivität von Dendryphiella arenaria. Chemie Ingenieur Technik 80:399–403

    Article  CAS  Google Scholar 

  • Kiseleva MI, Shevchenko NM, Krupnova TN, Zvyagintseva TN (2005) Effect of fucoidans on the developing embryos of the sea urchin Strongylocentrotus intermedius. J Evol Biochem Physiol 41:63–72

    Article  CAS  Google Scholar 

  • Kitamura K, Matsuo M, Yasui T (1992) Enzymatic degradation of fucoidan by fucoidanase from the hepatopancreas of Patinopecten yessoensis. Biosci Biotechnol Biochem 56:490–494

    Article  CAS  PubMed  Google Scholar 

  • Klarzynski O, Descamps V, Plesse B, Yvin J-C, Kloareg B, Fritig B (2003) Sulfated fucan oligosaccharides elicit defense responses in tobacco and local and systemic resistance against tobacco mosaic virus. Mol Plant Microb Interact 16:115–122

    Article  CAS  Google Scholar 

  • Kloareg B, Demarty M, Mabeau S (1986) Polyanionic characteristics of purified sulfated homofucans from brown algae. Int J Biol Macromol 8:380–386

    Article  CAS  Google Scholar 

  • Kusaykin MI, Burtseva YV, Svetasheva TG, Sova VV, Zvyagintseva TN (2003) Distribution of O-glycosylhydrolases in marine invertebrates. enzymes of the marine mollusk Littorina kurila that catalyze fucoidan transformation. Biochemistry (Moscow, Russian Federation)(Translation of Biokhimiya (Moscow, Russian Federation)) 68:317–324

    Article  CAS  Google Scholar 

  • Kuznetsova TA, Besednova NN, Mamaev AN, Momot AP, Shevchenko NM, Zvyagintseva TN (2003) Anticoagulant activity of fucoidan from brown algae Fucus evanescens of the Okhotsk Sea. Bull Exp Biol Med (Translation of Byulleten Eksperimental’noi Biologii i Meditsiny) 136:471–473

    CAS  Google Scholar 

  • Kylin H (1918) Biochemistry of seaweeds. Z Physiol Chem 101:236–247

    Article  CAS  Google Scholar 

  • Lapshina LA, Reunov AV, Nagorskaya VP, Zvyagintseva TN, Shevchenko NM (2006) Inhibitory effect of fucoidan from brown alga Fucus evanescens on the spread of infection induced by tobacco mosaic virus in tobacco leaves of two cultivars. Russ J Plant Physiol 53:246–251

    Article  CAS  Google Scholar 

  • Lapshina LA, Reunov AV, Nagorskaya VP, Zvyagintseva TN, Shevchenko NM (2007) Effect of fucoidan from brown alga Fucus evanescens on a formation of TMV-specific inclusions in the cells of tobacco leaves. Russ J Plant Physiol 54:111–114

    Article  CAS  Google Scholar 

  • Lee J-B, Hayashi K, Hashimoto M, Nakano T, Hayashi T (2004a) Novel antiviral fucoidan from sporophyll of Undaria pinnatifida (Mekabu). Chem Pharm Bull 52:1091–1094

    Article  CAS  Google Scholar 

  • Lee J-B, Hayashi K, Maeda M, Hayashi T (2004b) Antiherpetic activities of sulfated polysaccharides from green algae. Planta Med 70:813–817

    Article  CAS  PubMed  Google Scholar 

  • Li B, Wei X-J, Sun J-L, Xu S-Y (2006) Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed, Hizikia fusiforme. Carbohydr Res 341:1135–1146

    Article  CAS  PubMed  Google Scholar 

  • Mabeau S, Kloareg B, Joseleau JP (1990) Fractionation and analysis of fucans from brown algae. Phytochemistry 29:2441–2445

    Article  CAS  Google Scholar 

  • Masuko T, Minami A, Iwasaki N, Majima T, Nishimura S-I, Lee YC (2005) Carbohydrate analysis by a phenol–sulfuric acid method in microplate format. Anal Biochem 339:69–72

    Article  CAS  PubMed  Google Scholar 

  • Medcalf DG, Larsen B (1977) Fucose-containing polysaccharides in the brown algae Ascophyllum nodosum and Fucus vesiculosus. Carbohydr Res 59:531–537

    Article  CAS  Google Scholar 

  • Mian AJ, Percival E (1973a) Carbohydrates of the brown seaweeds Himanthalia lorea and Bifurcaria bifurcata. II. Structural studies of the fucans. Carbohydr Res 26:147–161

    Article  CAS  Google Scholar 

  • Mian AJ, Percival E (1973b) Carbohydrates of the brown seaweeds Himanthalia lorea, Bifurcaria bifurcata, and Padina pavonia. 1. Extraction and fractionation. Carbohydr Res 26:133–146

    Article  CAS  Google Scholar 

  • Miller GL (1959) Dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  • Min H, Cowman MK (1986) Combined Alcian blue and silver staining of glycosaminoglycans in polyacrylamide gels: application to electrophoretic analysis of molecular weight distribution. Anal Biochem 155:275–285

    Article  CAS  PubMed  Google Scholar 

  • Moeller HJ, Heinegaard D, Poulsen JH (1993) Combined Alcian blue and silver staining of subnanogram quantities of proteoglycans and glycosaminoglycans in sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem 209:169–175

    Article  CAS  Google Scholar 

  • Mulloy B (2002) Fucose-based polysaccharides of marine origin: research tools with therapeutic potential. In: Nantes Proceedings, pp 105–111

  • Mulloy B, Ribeiro A-C, Alves A-P, Vieira RP, Mourao PAS (1994) Sulfated fucans from echinoderms have a regular tetrasaccharide repeating unit defined by specific patterns of sulfation at the 0–2 and 0–4 positions. J Biol Chem 269:22113–22123

    CAS  PubMed  Google Scholar 

  • Nardella A, Chaubet F, Boisson-Vidal C, Blondin C, Durand P, Jozefonvicz J (1996) Anticoagulant low molecular wt. fucans produced by radical process and ion exchange chromatography of high molecular weight fucans extracted from the brown seaweed Ascophyllum nodosum. Carbohydr Res 289:201–208

    Article  CAS  PubMed  Google Scholar 

  • Nishino T, Nishioka C, Ura H, Nagumo T (1994) Isolation and partial characterization of a novel amino sugar-containing fucan sulfate from commercial Fucus vesiculosus fucoidan. Carbohydr Res 255:213–224

    Article  CAS  PubMed  Google Scholar 

  • Obluchinskaya ED, Minina SA (2004) Development of extraction technology and characterization of extract from wrack algae grist. Pharm Chem J (Translation of Khimiko-Farmatsevticheskii Zhurnal) 38:323–326

    CAS  Google Scholar 

  • Patankar MS, Oehninger S, Barnett T, Williams RL, Clark GF (1993) A revised structure for fucoidan may explain some of its biological activities. J Biol Chem 268:21770–21776

    CAS  PubMed  Google Scholar 

  • Percival EGV, Ross AG (1950) Fucoidin. I. The isolation and purification of fucoidin from brown seaweeds. J Chem Soc 145:717–720

    Article  Google Scholar 

  • Pereira MS, Mulloy B, Mourao PAS (1999) Structure and anticoagulant activity of sulfated fucans. Comparison between the regular, repetitive, and linear fucans from echinoderms with the more heterogeneous and branched polymers from brown algae. J Biol Chem 274:7656–7667

    Article  CAS  PubMed  Google Scholar 

  • Pomin VH, Pereira MS, Valente A-P, Tollefsen DM, Pavao MSG, Mourao PAS (2005) Selective cleavage and anticoagulant activity of a sulfated fucan: stereospecific removal of a 2-sulfate ester from the polysaccharide by mild acid hydrolysis, preparation of oligosaccharides, and heparin cofactor II-dependent anticoagulant activity. Glycobiology 15:369–381

    Article  PubMed  Google Scholar 

  • Ponce NMA, Pujol CA, Damonte EB, Flores ML, Stortz CA (2003) Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity and structural studies. Carbohydr Res 338:153–165

    Article  CAS  PubMed  Google Scholar 

  • Preeprame S, Hayashi K, Lee J-B, Sankawa U, Hayashi T (2001) A novel antivirally active fucan sulfate derived from an edible brown alga, Sargassum horneri. Chem Pharm Bull 49:484–485

    Article  CAS  Google Scholar 

  • Qiu X, Amarasekara A, Doctor V (2006) Effect of oversulfation on the chemical and biological properties of fucoidan. Carbohydr Polym 63:224–228

    Article  CAS  Google Scholar 

  • Ribeiro AC, Vieira RP, Mourao PAS, Mulloy B (1994) A sulfated alpha-L-fucan from sea cucumber. Carbohydr Res 255:225–240

    Article  CAS  PubMed  Google Scholar 

  • Ruperez P, Ahrazem O, Leal JA (2002) Potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed Fucus vesiculosus. J Agric Food Chem 50:840–845

    Article  CAS  PubMed  Google Scholar 

  • Sakai T, Kimura H, Kato I (2002) A marine strain of Flavobacteriaceae utilizes brown seaweed fucoidan. Mar Biotechnol 4:399–405

    Article  CAS  Google Scholar 

  • Sakai T, Ishizuka K, Kato I (2003a) Isolation and characterization of a fucoidan-degrading marine bacterium. Mar Biotechnol 5:409–416

    Article  CAS  Google Scholar 

  • Sakai T, Ishizuka K, Shimanaka K, Ikai K, Kato I (2003b) Structures of oligosaccharides derived from Cladosiphon okamuranus fucoidan by digestion with marine bacterial enzymes. Mar Biotechnol 5:536–544

    Article  CAS  Google Scholar 

  • Sakai T, Kimura H, Kato I (2003c) Purification of sulfated fucoglucuronomannan lyase from bacterial strain of Fucobacter marina and study of appropriate conditions for its enzyme digestion. Mar Biotechnol 5:380–387

    Article  CAS  Google Scholar 

  • Sakai T, Kimura H, Kojima K, Shimanaka K, Ikai K, Kato I (2003d) Marine bacterial sulfated fucoglucuronomannan (SFGM) lyase digests brown algal SFGM into trisaccharides. Mar Biotechnol 5:70–78

    Article  CAS  Google Scholar 

  • Sakai T, Kawai T, Kato I (2004) Isolation and characterization of a fucoidan-degrading marine bacterial strain and its fucoidanase. Mar Biotechnol 6:335–346

    Article  CAS  Google Scholar 

  • Sasaki K, Sakai T, Kojima K, Nakayama S, Nakanishi Y, Kato I (1996) Partial purification and characterization of an enzyme releasing 2-sulfo-alpha-L-fucopyranose from 2-sulfo-alpha-L-fucopyranosyl-(1->2)pyridylaminated fucose from a sea urchin, Strongylocentrotus nudus. Biosci Biotechnol Biochem 60:666–668

    Article  CAS  Google Scholar 

  • Senni K et al (2006) Fucoidan a sulfated polysaccharide from brown algae is a potent modulator of connective tissue proteolysis. Arch Biochem Biophys 445:56–64

    Article  CAS  PubMed  Google Scholar 

  • Shevchenko NM, Anastyuk SD, Gerasimenko NI, Dmitrenok PS, Isakov VV, Zvyagintseva TN (2007) Polysaccharide and lipid composition of the brown seaweed Laminaria gurjanovae. Russ J Bioorgan Chem 33:88–98

    Article  CAS  Google Scholar 

  • Siddhanta AK, Murthy ASK (2001) Bioactive polysaccharides from marine brown algae (Phaeophyceae). J Indian Chem Soc 78:431–437

    CAS  Google Scholar 

  • Silva TMA (2005) Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora. Braz J Med Biol Res 38:523–533 Revista brasileira de pesquisas medicas e biologicas/Sociedade Brasileira de Biofisica... [et al.]

    Article  CAS  PubMed  Google Scholar 

  • Skibola Christine F (2004) The effect of Fucus vesiculosus, an edible brown seaweed, upon menstrual cycle length and hormonal status in three pre-menopausal women: a case report. BMC Complement Altern Med 4:10

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23

    CAS  Google Scholar 

  • Suppiramaniam V, Vaithianathan T, Manivannan K, Dhanasekaran M, Parameshwaran K, Bahr BA (2006) Modulatory effects of dextran sulfate and fucoidan on binding and channel properties of AMPA receptors isolated from rat brain. Synapse (Hoboken, NJ, United States) 60:456–464

    Article  CAS  Google Scholar 

  • Takayama M, Koyama N, Sakai T, Kato I (2002) Enzymes capable of degrading a sulfated-fucose-containing polysaccharide and their encoding genes. US Patent 6489155

  • Tako M, Yoza E, Tohma S (2000) Chemical characterization of acetyl fucoidan and alginate from commercially cultured Cladosiphon okamuranus. Bot Mar 43:393–398

    Article  CAS  Google Scholar 

  • Thanassi NM, Nakada HI (1967) Enzymic degradation of fucoidan by enzymes from the hepatopancreas of abalone, Haliotus species. Arch Biochem Biophys 118:172–177

    Article  CAS  Google Scholar 

  • Thorlacius H, Vollmar B, Seyfert UT, Vestweber D, Menger MD (2000) The polysaccharide fucoidan inhibits microvascular thrombus formation independently from P- and L-selectin function in vivo. Eur J Clin Investig 30:804–810

    Article  CAS  Google Scholar 

  • Tissot B, Daniel R (2003) Biological properties of sulfated fucans: the potent inhibiting activity of algal fucoidan against the human complement system. Glycobiology 13:29G–30G

    Article  CAS  PubMed  Google Scholar 

  • Tissot B, Salpin J-Y, Martinez M, Gaigeot M-P, Daniel R (2006) Differentiation of the fucoidan sulfated L-fucose isomers constituents by CE-ESIMS and molecular modeling. Carbohydr Res 341:598–609

    Article  CAS  PubMed  Google Scholar 

  • Urvantseva AM, Bakunina IY, Nedashkovskaya OI, Kim SB, Zvyagintseva TN (2006) Distribution of intracellular fucoidan hydrolases among marine bacteria of the family Flavobacteriaceae. Appl Biochem Microbiol 42:484–491

    Article  CAS  Google Scholar 

  • Vilela-Silva A-CES, Alves A-P, Valente A-P, Vacquier VD, Mourao PAS (1999) Structure of the sulfated alpha-L-fucan from the egg jelly coat of the sea urchin Strongylocentrotus franciscanus: patterns of preferential 2-O- and 4-O-sulfation determine sperm cell recognition. Glycobiology 9:927–933

    Article  CAS  PubMed  Google Scholar 

  • Volpi N, Maccari F (2006) Electrophoretic approaches to the analysis of complex polysaccharides. J Chromatogr B Analyt Technol Biomed Life Sci 834:1–13

    Article  CAS  PubMed  Google Scholar 

  • Wardi AH, Allen WS (1972) Alcian blue staining of glycoproteins. Anal Biochem 48:621–623

    Article  CAS  PubMed  Google Scholar 

  • Witvrouw M, De Clercq E (1997) Sulfated polysaccharides extracted from sea algae as potential antiviral drugs. Gen Pharmacol 29:497–511

    Article  CAS  PubMed  Google Scholar 

  • Wu K et al (2002) Production of fucoidanase from marine fungus Dendryphiella arenaria TM 94 by solid substrate fermentation using marine algae resources. Nantes Proceedings, pp 122–133

  • Xue C-H et al (2001) Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica. J Appl Phycol 13:67–70

    Article  CAS  Google Scholar 

  • Yaphe W, Morgan K (1959) Enzymic hydrolysis of fucoidin by Pseudomonas atlantica and Pseudomonas carrageenovora. Nature (London, United Kingdom) 183:761–762

    Article  CAS  Google Scholar 

  • Ye J et al (2005) Enzyme-digested fucoidan extracts derived from seaweed Mozuku of Cladosiphon novae-caledoniae kylin inhibit invasion and angiogenesis of tumor cells. Cytotechnology 47:117–126

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhang Q, Li N, Zhao T, Qi H, Xu Z, Li Z (2005) Fucoidan inhibits the development of proteinuria in active Heymann nephritis. Phytotherapy Research 19:50–53

    Article  CAS  PubMed  Google Scholar 

  • Zvyagintseva TN et al (1999) A new procedure for the separation of water-soluble polysaccharides from brown seaweeds. Carbohydr Res 322:32–39

    Article  CAS  Google Scholar 

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Acknowledgement

The authors would like to thank very much the German Research Association (DFG) for kind financial support of a project on the search for and use of enzymes of marine origin for the hydrolysis of sulphated polysaccharides.

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Correspondence to Siegmund Lang.

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Holtkamp, A.D., Kelly, S., Ulber, R. et al. Fucoidans and fucoidanases—focus on techniques for molecular structure elucidation and modification of marine polysaccharides. Appl Microbiol Biotechnol 82, 1–11 (2009). https://doi.org/10.1007/s00253-008-1790-x

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  • DOI: https://doi.org/10.1007/s00253-008-1790-x

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