Abstract
A mutant Aspergillus carbonarius selected for temperature tolerance after UV treatment, when grown in shake flasks, produced mycelia bearing yellow pigment. Since the mutant was affected in sterol biosynthetic pathway, the pigment was apparently produced to maintain membrane fluidity and rigidity for growth sustenance in low-pH culture broth. Nuclear magnetic resonance analyses characterizing the pigment as a partially saturated canthaxanthin, containing β-ionone end rings, suggested its application as a retinoid. When tested for this property in retinoic acid receptor expressing prostate cancer cell line, LNCaP, the fungal partially saturated canthaxanthin induced apoptosis. Low apoptosis percentage in DU145 prostrate cancer cells that does not express functional retinoic acid receptor-β (RAR-β) suggested binding specificity of the partially saturated canthaxanthin for RAR-β.
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Acknowledgement
The authors thank Dr. Dinesh K Sukumaran, Director, Magnetic Resonance Center, Department of Chemistry, State University of New York, Buffalo, NY, USA for his assistance in NMR data and its analysis. NK, KRS, KSV, and RK were supported by fellowship grants from Indian Government research agencies, the Council of Scientific Industrial Research, Indian Council of Medical Research and University Grant Commission.
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An erratum to this article can be found at https://doi.org/10.1007/s00253-008-1586-z
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Kumaresan, N., Sanjay, K.R., Venkatesh, K.S. et al. Partially saturated canthaxanthin purified from Aspergillus carbonarius induces apoptosis in prostrate cancer cell line. Appl Microbiol Biotechnol 80, 467–473 (2008). https://doi.org/10.1007/s00253-008-1538-7
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DOI: https://doi.org/10.1007/s00253-008-1538-7