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Isolation and characterization of a novel Burkholderia cepacia with strong antifungal activity against Rhizoctonia solani

  • Applied Microbial and Cell Physiology
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Abstract

Strain CF-66 with strong antifungal activity against Rhizoctonia solani was isolated from compost samples. It is clearly demonstrated that strain CF-66 is belonging to Burkholderia cepacia complex by the morphological and biochemical tests and 16S rDNA sequence. The B. cepacia complex consists of a group of bacteria currently organized into nine genomovars, among them genomovar II and genomovar III, contain the highly epidemic strains. However, it was known that strain CF-66 is not a member of genomovar II or III of the B. cepacia complex by species-specific polymerase chain reaction assay. In this study, the antifungal compound CF66I produced by strain CF-66 was purified and characterized. Based on the nuclear magnetic resonance, GC-MS spectral and infrared spectral data, CF66I was confirmed to have amide bonds, α-metyl fatty acid, bromine, and some structural units such as CH2CH2O. CF66I is stable to high temperature, proteolytic enzymes, and organic solvents. CF66I inhibit the growth of a variety of plant pathogenic fungi and pathogenic yeast, whereas bacterial cells are unaffected. CF66I mainly reduced hyphal extension rates in a dose-dependent manner and induced severe change in cell morphology that resulted in swelled and formed very short hyphae with multiple branches.

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Authors and Affiliations

  1. Life Science College, Dalian Nationalities University, Economical and Technological Development Zone, Dalian, 116600, People’s Republic of China

    C. S. Quan, W. Zheng, Q. Liu & S. D. Fan

  2. Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, 739-8528, Japan

    Y. Ohta

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  1. C. S. Quan
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  2. W. Zheng
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  3. Q. Liu
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  4. Y. Ohta
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  5. S. D. Fan
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Correspondence to S. D. Fan.

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Quan, C.S., Zheng, W., Liu, Q. et al. Isolation and characterization of a novel Burkholderia cepacia with strong antifungal activity against Rhizoctonia solani . Appl Microbiol Biotechnol 72, 1276–1284 (2006). https://doi.org/10.1007/s00253-006-0425-3

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  • DOI: https://doi.org/10.1007/s00253-006-0425-3

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