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Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene

  • Applied Genetics and Molecular Biotechnology
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Abstract

Penicillin G acylase (PGA; E.C. 3.5.1.11) is an important enzyme which has broad applications in industries of β-lactim antibiotics production. In this study, a promising PGA gene from Alcaligenes faecalis (afpga) and another pcm gene encoding protein isoaspartate methyltransferase (PIMT) were constructed into pET43.1a(+) and pET28a(+), respectively. The recombinant plasmids pETAFPGA and pETPCM were transformed into the same host cell Escherichia coli BL21 (DE3). Results suggested that the two plasmids could peacefully exist in the host cell and the two genes could be efficiently expressed after induction. The product of pcm gene could function as a helper molecule for enzyme AFPGA. PIMT increased the enzymatic activities in supernatant of ferment broth (1.6 folds) and cell lysate (1.8 folds), while it did not significantly affect the expression level of penicillin G acylase.

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Acknowledgement

This work was financially supported by a grant from the National High Technology Research and Development Program of China (No.2002AA2Z345A).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China

    Tianwen Wang, Hu Zhu, Xingyuan Ma, Zhuoya Fei, Yushu Ma & Dongzhi Wei

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  1. Tianwen Wang
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  2. Hu Zhu
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  3. Xingyuan Ma
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  4. Zhuoya Fei
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  5. Yushu Ma
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  6. Dongzhi Wei
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Correspondence to Dongzhi Wei.

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Wang, T., Zhu, H., Ma, X. et al. Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene. Appl Microbiol Biotechnol 72, 953–958 (2006). https://doi.org/10.1007/s00253-006-0349-y

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  • DOI: https://doi.org/10.1007/s00253-006-0349-y

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