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Cloning and expression of a gene for an alpha-glucosidase from Saccharomycopsis fibuligera homologous to family GH31 of yeast glucoamylases

  • Applied Genetics and Molecular Biotechnology
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Abstract

Cloning of cDNA encoding an α-glucosidase from the dimorphous yeast Saccharomycopsis fibuligera and characterization of the gene product were performed. The cDNA of the putative α-glucosidase gene consists of 2,886 bp, which includes an open reading frame encoding a 19 amino acid signal peptide at the N-terminal end and a 944 amino acid mature protein with a predicted molecular mass of 105.4 kDa and pI value of 4.52. The deduced amino acid sequence shows a high degree of identity (70%) with two yeast glucoamylases, namely, the extracellular glucoamylase Gam from Schwanniomyces occidentalis and the cell surface glucoamylase Gca from Candida albicans. The recombinant product, synthesized in Saccharomyces cerevisiae, is localized on the cell surface and hydrolyses maltooligosaccharides exclusively without the ability to digest soluble starch, which is consistent with the specificity characteristic of α-glucosidase, EC. 3.2.1.20.

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Acknowledgements

This work was supported by grants 2/1092/21 and 1/010/03 from the Slovak Grant Agency VEGA.

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Authors and Affiliations

  1. Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51, Bratislava, Slovakia

    Eva Hostinová, Adriana Solovicová & Juraj Gašperík

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  1. Eva Hostinová
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  2. Adriana Solovicová
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  3. Juraj Gašperík
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Correspondence to Eva Hostinová.

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Hostinová, E., Solovicová, A. & Gašperík, J. Cloning and expression of a gene for an alpha-glucosidase from Saccharomycopsis fibuligera homologous to family GH31 of yeast glucoamylases. Appl Microbiol Biotechnol 69, 51–56 (2005). https://doi.org/10.1007/s00253-005-1971-9

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  • DOI: https://doi.org/10.1007/s00253-005-1971-9

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