Abstract
Agrobacterium tumefaciens ATCC4452 cells were irradiated by nitrogen ion beam, a new mutagen, with energy of 10 keV and fluence ranging from 2.6×1014 ions/cm2 to 6.5×1015 ions/cm2. A similar “saddle shape” survival curve due to ion beam irradiation appeared again in this study. Some mutants with high yield of ubiquinone-10 were induced by ion implantation. High mutation rate and wide mutation spectrum were also observed in the experiment. These results suggested that the mutagenic effect of such low-energy ion influx into bacterium cells could result from multiple processes involving direct collision of particles with cytoplasm, nucleolus, and cascade atomic and molecular reactions due to plentiful primary and secondary particles.
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Acknowledgements
This work is sponsored by the national natural science foundation committee of china under contract No.20576132. The authors are indebted to the others of research group of Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, for their excellent operation and helpful discussion.
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Gu, SB., Yao, JM., Yuan, QP. et al. A novel approach for improving the productivity of ubiquinone-10 producing strain by low-energy ion beam irradiation. Appl Microbiol Biotechnol 72, 456–461 (2006). https://doi.org/10.1007/s00253-005-0283-4
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DOI: https://doi.org/10.1007/s00253-005-0283-4