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Isolation and transcriptional analysis of novel tetrachloroethene reductive dehalogenase gene from Desulfitobacterium sp. strain KBC1

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Abstract

Strain KBC1, an anaerobic bacterium, that dechlorinates tetrachloroethene (PCE) to trichloroethene was isolated. This strain also dechlorinated high concentrations of PCE at a temperature range of 10 to 40°C and showed high oxygen tolerance. Based on the 16S rRNA gene sequence analysis, this microorganism was identified as a species of the genus Desulfitobacterium. Several species of this genus have been reported to be potent ortho-chlorophenol and PCE dechlorinators; however, the gene coding PCE-specific dehalogenase had not been cloned thus far. In this report, we identified a novel PCE reductive dehalogenase (PrdA) gene from the Desulfitobacterium sp. strain KBC1. These prd genes, including putative membrane anchor protein, were classified as novel type of PCE reductive dehalogenase (approximately 40% homology with the general PCE dehalogenase). It was revealed that the two open reading frames had been transcribed as identical mRNA and were induced strictly in the presence of PCE. This transcriptional regulation appeared to be controlled by the transcriptional activator located downstream of prdAB operon. According to the substrate utility of the strain KBC1 and phylogenetic analysis of PrdA, this microorganism may be expected to play the role of a primary dechlorinator of PCE in the environment.

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Acknowledgements

We thank Yoichi Kamagata and Naoya Shinzato (National Institute of Advanced Industrial Science and Technology) for advice on isolation of strain KBC1.

This study was carried out as a part of “The Project for Development of Technologies for Analyzing and Controlling the Mechanism of Biodegrading and Processing,” which was entrusted by the New Energy and Industrial Technology Development Organization (NEDO).

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Authors and Affiliations

  1. Biotechnology Research Center, KUBOTA Corporation, Osaka, Japan

    Norihiko Tsukagoshi, Satoshi Ezaki, Tetsuya Uenaka, Nobukazu Suzuki & Ryuichiro Kurane

  2. 5-6, Koyodai, Ryugasaki City, Ibaraki, 301-0852, Japan

    Norihiko Tsukagoshi

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  1. Norihiko Tsukagoshi
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  2. Satoshi Ezaki
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  3. Tetsuya Uenaka
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  4. Nobukazu Suzuki
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Correspondence to Norihiko Tsukagoshi.

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Tsukagoshi, N., Ezaki, S., Uenaka, T. et al. Isolation and transcriptional analysis of novel tetrachloroethene reductive dehalogenase gene from Desulfitobacterium sp. strain KBC1. Appl Microbiol Biotechnol 69, 543–553 (2006). https://doi.org/10.1007/s00253-005-0022-x

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