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Anaerobic oxidation of 2-chloroethanol under denitrifying conditions by Pseudomonas stutzeri strain JJ

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Abstract

A bacterium that uses 2-chloroethanol as sole energy and carbon source coupled to denitrification was isolated from 1,2-dichloroethane-contaminated soil. Its 16 S rDNA sequence showed 98% similarity with the type strain of Pseudomonas stutzeri (DSM 5190) and the isolate was tentatively identified as Pseudomonas stutzeri strain JJ. Strain JJ oxidized 2-chloroethanol completely to CO2 with NO3 − or O2 as electron acceptor, with a preference for O2 if supplied in combination. Optimum growth on 2-chloroethanol with nitrate occurred at 30 °C with a µ max of 0.14 h−1 and a yield of 4.4 g protein per mol 2-chloroethanol metabolized. Under aerobic conditions, the µ max was 0.31 h−1. NO2 − also served as electron acceptor, but reduction of Fe(OH)3, MnO2, SO4 2−, fumarate or ClO3 − was not observed. Another chlorinated compound used as sole energy and carbon source under aerobic and denitrifying conditions was chloroacetate. Various different bacterial strains, including some closely related Pseudomonas stutzeri strains, were tested for their ability to grow on 2-chloroethanol as sole energy and carbon source under aerobic and denitrifying conditions, respectively. Only three strains, Pseudomonas stutzeri strain LMD 76.42, Pseudomonas putida US2 and Xanthobacter autotrophicus GJ10, grew aerobically on 2-chloroethanol. This is the first report of oxidation of 2-chloroethanol under denitrifying conditions by a pure bacterial culture.

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Acknowledgements

We thank Harald Ruijssenaars (Hercules European Research Center) and Hans Visser (Wageningen University) for help with cloning the 16S rDNA. We also thank Dick B. Janssen, who kindly provided bacterial strains. The research described in this paper was supported by and carried out at the Research Centre on Soil, Sediment and Groundwater Management and Remediation WUR/TNO.

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Authors and Affiliations

  1. Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT, Wageningen, The Netherlands

    J. A. Dijk, A. J. M. Stams & G. Schraa

  2. TNO Environment, Energy and Process Innovation, P.O. Box 342, 7300 AH, Apeldoorn, The Netherlands

    H. Ballerstedt, J. A. M. de Bont & J. Gerritse

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  1. J. A. Dijk
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  2. A. J. M. Stams
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  3. G. Schraa
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  4. H. Ballerstedt
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  5. J. A. M. de Bont
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  6. J. Gerritse
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Correspondence to J. A. Dijk.

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Dijk, J.A., Stams, A.J.M., Schraa, G. et al. Anaerobic oxidation of 2-chloroethanol under denitrifying conditions by Pseudomonas stutzeri strain JJ. Appl Microbiol Biotechnol 63, 68–74 (2003). https://doi.org/10.1007/s00253-003-1346-z

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  • DOI: https://doi.org/10.1007/s00253-003-1346-z

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