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Characterization of Aspergillus oryzae fermentation extract effects on the rumen fungus Neocallimastix frontalis, EB 188. Part 1. Zoospore development and physiology

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Abstract

Experiments were performed to determine the effect of Aspergillus oryzae (AO) fermentation extract on zoospore development in the rumen fungus Neocallimastix frontalis EB 188. Powdered product, or liquid extract prepared from such powder, was added at the recommended value for supplementation in dairy cattle. Stationary and stirred cultures were periodically sampled and assayed for extracellular and intracellular protein and enzymes, gas production, zoospore production and maturation, and carbon source utilization. Soluble extract increased fungal physiology when grown in stirred vessels or stationary cultures. Treated cultures produced higher levels of enzymes (nearly double). Mobile zoospores matured into germination entities more rapidly in treated cultures, and when powdered product was used, nearly 3 times more motile zoospores were produced at 56 h of fungal growth. Levels of the intracellular enzyme malate dehydrogenase increased by 6-fold in the presence of powdered product. Product wheat bran carrier used as soluble extract or powder had very little effect on fungal cultures. Medium cellulose was completely hydrolyzed in all cultures but this occurred earlier in those containing AO treatment.

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Acknowledgements

The authors wish to thank the Agricultural Research Center at Washington State University, the Washington State Dairy Commission, and BioZyme Incorporated, (St. Joseph, Mo.) for financial support of this research. We also extend our appreciation for the experimental assistance of M. Robertson and M.S. Cheong. Lastly, we are most grateful for the critical review of the manuscript provided by colleagues at Washington State University.

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  1. Department of Animal Sciences and School of Molecular BioSciences, Washington State University, Pullman, WA 99164–6353, USA

    J. A. Schmidt, S. Albright, K.-P. Tsai, G. M. Calza, J. S. Chang & R. E. Calza

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  1. J. A. Schmidt
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  2. S. Albright
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  3. K.-P. Tsai
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  4. G. M. Calza
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  5. J. S. Chang
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  6. R. E. Calza
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Correspondence to R. E. Calza.

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Schmidt, J.A., Albright, S., Tsai, KP. et al. Characterization of Aspergillus oryzae fermentation extract effects on the rumen fungus Neocallimastix frontalis, EB 188. Part 1. Zoospore development and physiology. Appl Microbiol Biotechnol 63, 422–430 (2004). https://doi.org/10.1007/s00253-003-1293-8

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  • DOI: https://doi.org/10.1007/s00253-003-1293-8

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