Abstract.
Current research into poly-γ-glutamate (PGA) and its biosynthesis is reviewed. In PGA-producing Bacillus subtilis, glutamate racemase supplies abundant DL-glutamate, the substrate for PGA synthesis. The pgsBCA genes of PGA-producing B. subtilis, which encode the membrane-associated PGA synthetase complex PgsBCA, were characterized and the enzyme complex was suggested to be an atypical amide ligase based on its structure and function. A novel reaction mechanism of PGA synthesis is proposed.
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Ashiuchi, .M., Misono, .H. Biochemistry and molecular genetics of poly-γ-glutamate synthesis. Appl Microbiol Biotechnol 59, 9–14 (2002). https://doi.org/10.1007/s00253-002-0984-x
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DOI: https://doi.org/10.1007/s00253-002-0984-x