Abstract
Sooty mangabeys (Cercocebus atys) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roche/454 and Illumina MiSeq deep sequencing in order to determine the MHC class I transcripts in a cohort of 165 sooty mangabeys from the Yerkes National Primate Research Center (YNPRC). We have characterized 121 functionally full-length classical (Ceat-A and Ceat-B) and non-classical (Ceat-F and Ceat-I) alleles and have also identified 22 Ceat-A/Ceat-B haplotype chromosomal combinations. We correlated these Ceat-A/Ceat-B haplotype combinations to recently described microsatellite haplotypes from the YNPRC colony. These newly identified alleles and haplotypes establish a resource for studying cellular immunity in sooty mangabeys and provide a framework for rapidly cataloging MHC class I sequences in an understudied, yet important, nonhuman primate species.
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Acknowledgments
This work was supported by the National Institute of Allergy and Infectious Diseases (HHSN272201100013C), the National Center for Research Resources (R24 RR021745), the Wisconsin National Primate Research Center Base Grant from the National Center for Research Resources (P51 RR000167), and the Office of Research Infrastructure Programs (P51 OD011106) of the National Institutes of Health. This research was conducted at a facility constructed with support from the Research Facilities Improvement Project (RR15450-01, RR020141-01). This work was also supported by funding by National Institutes of Health grants P51 OD0111103 to NEPRC and P51 OD011132 to YNPRC. We gratefully acknowledge the help of Tracy Meeker, Stephanie Ehnert, and Kay Lee Summerville at YNPRC for the assistance with collection and shipping of samples from mangabeys.
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Heimbruch, K.E., Karl, J.A., Wiseman, R.W. et al. Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys. Immunogenetics 67, 437–445 (2015). https://doi.org/10.1007/s00251-015-0847-0
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DOI: https://doi.org/10.1007/s00251-015-0847-0