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Chicken MHC class I and II gene effects on antibody response kinetics in adult chickens

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Abstract

The major histocompatibility complex (MHC) plays an important role in regulation of the immune response. The MHC class I and II genes were selected as candidates to investigate associations with vaccine response to Salmonella enteritidis and kinetics of antibody response to sheep red blood cell (SRBC) and Brucella abortus. Primary antibody response after S. enteritidis vaccination at day 10, and antibody response to SRBC and killed B. abortus after immunization at 19 and 22 weeks were measured in an F2 population. The resource population was derived from males of two highly inbred MHC-congenic Fayoumi chicken lines (M5.1 and M15.2) mated with highly inbred G-B1 Leghorn line hens. Secondary phase parameters of minimum titers (Ymin), maximum titers (Ymax), and time needed to achieve Ymin (tmin) and Ymax (tmax) were estimated from post-secondary titers by using a non-linear regression model. Associations of single nucleotide polymorphisms (SNPs) in MHC class I and II genes with antibody response parameters were determined by a general linear model. Significant associations were found primarily in the M15.2 grandsire haplotype. There were significant associations between MHC class I α1 and α2 SNPs and antibody response to S. enteritidis, primary antibody response to B. abortus, Ymin to SRBC, and Ymax to both SRBC and B. abortus. There were significant effects of the MHC class II β1 domain SNP on S. enteritidis antibody and Ymax to SRBC. The results suggest that the characterized SNPs might be used in future applications by marker-assisted selection to improve vaccine response and immunocompetence in chickens.

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Fig. 2A, B.

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Acknowledgements

The authors thank Wei Liu for excellent technical assistance. We thank IDEXX Laboratories for donation of some of the ELISA kits for this study. This journal paper of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project No. 2576 and 3521, was supported by Hatch Act, Animal Health and State of Iowa funds. The experiments comply with the current laws of the USA.

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  1. Department of Animal Science, Iowa State University, Ames, IA 50011-3150, USA

    Huaijun Zhou & Susan J. Lamont

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  1. Huaijun Zhou
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  2. Susan J. Lamont
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Correspondence to Susan J. Lamont.

Additional information

The nucleotide sequence data reported in this paper have been submitted to the EMBL/GenBank nucleotide sequence databases and assigned the accession numbers AY210420–AY210424

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Zhou, H., Lamont, S.J. Chicken MHC class I and II gene effects on antibody response kinetics in adult chickens. Immunogenetics 55, 133–140 (2003). https://doi.org/10.1007/s00251-003-0566-9

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  • DOI: https://doi.org/10.1007/s00251-003-0566-9

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