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The Role of Biogeography in Shaping Intestinal Flora and Influence on Fatty Acid Composition in Red Swamp Crayfish (Procambarus clarkii)

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Abstract

Intestinal microbiota plays an important role in promoting digestion, metabolism, and immunity. Intestinal microbiota and fatty acids are important indicators to evaluate the health and nutritional composition of Procambarus clarkii. They have been shown to be strongly influence by environmental and genetic factors. However, it is not clear whether environmental factors have a greater impact on the intestinal microbiota and fatty acid composition of crayfish. The link between the intestinal microbial communities and fatty acid (FA) compositions of red swamp crayfish from different geographical has not yet been studied. Thus, the current paper focuses on the influence of different environments on the fatty acids in muscles of crayfish and the possible existence between gut microbiota and fatty acids. Therefore, in this study, we compared the fatty acid compositions and intestinal microbiota of five crayfish populations from different geographical locations. The results were further analyzed to determine whether there is a relationship between geographical location, fatty acid compositions and intestinal microbiota. The gut microbial communities of the crayfish populations were characterized using 16S rRNA high-throughput gene sequencing. The results showed that there were significant differences in FA compositions of crayfish populations from different geographical locations. A similar trend was observed in the gut microbiome, which also varied significantly according to geographic location. Interestingly, the analysis revealed that there was a relationship between fatty acid compositions and intestinal microbes, revealed by alpha diversity analysis and cluster analysis. However, further studies of the interactions between the P. clarkii gut microbiota and biochemical composition are needed, which will ultimately reveal the complexity of microbial ecosystems with potential applications in aquaculture and species conservation.

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Data Availability

The datasets presented in this study are available in online repositories. The names of the repository/repositories and accession number(s) can be found below. https://dataview.ncbi.nlm.nih.gov/object/PRJNA1018237.

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Acknowledgements

We thank the students and staff of the Aquatic Genetic Laboratory, FFRC for their assistance in this study.

Funding

This work was supported by grants from the “JBGS” Project of Seed Industry Revitalization in Jiangsu Province (JBGS [2021]123), Central Public-interest Scientific Institution Basal Research Fund, CAFS (NO. 2022XT01), Central Public-interest Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS (NO. 2023JBFM21).

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Author notes

  1. Ming Chen and Shengyan Su contributed equally.

Authors and Affiliations

  1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China

    Ming Chen, Shengyan Su, Chengfeng Zhang, Jian Zhu, Jingjing Jiang & Zeyu Lu

  2. Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China

    Shengyan Su, Chengfeng Zhang, Jian Zhu, Wenrong Feng, Wenting Liu & Jingchang Gan

  3. Jiangsu Fishery Technology Promotion Center, Jiangsu, China

    Huangen Chen

Authors
  1. Ming Chen
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Contributions

Shengyan Su conceived the study and contributed to the design of the experiments. Ming Chen, Wenrong Feng, Can Tian, Jingjing Jiang, Yang Gao, Zeyu Lu, Jian zhu, Chengfeng Zhang performed all the experiments. All authors contributed to the drafting of the manuscript.

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Correspondence to Shengyan Su.

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Chen, M., Su, S., Zhang, C. et al. The Role of Biogeography in Shaping Intestinal Flora and Influence on Fatty Acid Composition in Red Swamp Crayfish (Procambarus clarkii). Microb Ecol 86, 3111–3127 (2023). https://doi.org/10.1007/s00248-023-02298-4

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