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Emergence of a Synergistic Diversity as a Response to Competition in Pseudomonas putida Biofilms

  • Environmental Microbiology
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Abstract

Genetic diversification through the emergence of variants is one of the known mechanisms enabling the adaptation of bacterial communities. We focused in this work on the adaptation of the model strain Pseudomonas putida KT2440 in association with another P. putida strain (PCL1480) recently isolated from soil to investigate the potential role of bacterial interactions in the diversification process. On the basis of colony morphology, three variants of P. putida KT2440 were obtained from co-culture after 168 h of growth whereas no variant was identified from the axenic KT2440 biofilm. The variants exhibited distinct phenotypes and produced biofilms with specific architecture in comparison with the ancestor. The variants better competed with the P. putida PCL1480 strain in the dual-strain biofilms after 24 h of co-culture in comparison with the ancestor. Moreover, the synergistic interaction of KT2440 ancestor and the variants led to an improved biofilm production and to higher competitive ability versus the PCL1480 strain, highlighting the key role of diversification in the adaptation of P. putida KT2440 in the mixed community. Whole genome sequencing revealed mutations in polysaccharides biosynthesis protein, membrane transporter, or lipoprotein signal peptidase genes in variants.

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Acknowledgments

Claus Sternberg is acknowledged for the kind gift of the P. putida KT2440 GFP strain and Ellen Lagendijk for the P.putida PCL1480 mCherry strain.

Funding

This work was funded by the French National Research Agency (ANR) within the SYSCOMM project DISCO (ANR-09-SYSC-003). This study was conducted on the LABE experimental platform funded by DRRT in the framework of CPER 2007–2013 projects.

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  1. ANSES, Fougères Laboratory, AB2R, 10B rue Claude Bourgelat, 35300, Fougères, France

    Arnaud Bridier

  2. IRSTEA, UR PROSE, 1 rue Pierre-Gilles de Gennes, 92761, Antony Cedex, France

    Arnaud Bridier & T. Bouchez

  3. Institut Micalis, INRA, AgroParisTech, Université Paris-Saclay, 78350, Jouy-en-Josas, France

    J. C. Piard & R. Briandet

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  1. Arnaud Bridier
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Correspondence to Arnaud Bridier.

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Bridier, A., Piard, J.C., Briandet, R. et al. Emergence of a Synergistic Diversity as a Response to Competition in Pseudomonas putida Biofilms. Microb Ecol 80, 47–59 (2020). https://doi.org/10.1007/s00248-019-01470-z

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