Abstract
The diversity of heterotrophic bacterial isolates of three commercial and two homemade composts was studied. The commercial composts were produced from poultry litter (PC), sewage sludge (SC), municipal solid waste (MC), and homemade composts (thermal compost [DC] and vermicompost [VC]) from food wastes. The taxonomic and physiological diversity of the heterotrophic culturable bacteria was assessed using phenotypic and genotypic characterization and the analysis of the partial 16S rRNA gene sequence. Composts DC and SC presented the higher genotypic diversity, as could be inferred from the number of distinct genotypic patterns observed, 28 and 21, respectively. Gram-positive bacteria, mainly Firmicutes, were predominant in all the composts. Some organisms related with taxa rarely reported in composts, as Rhodanobacter spathiphylli, Moraxella osloensis, Lysobacter, Corynebacterium, Pigmentiphaga kullae, and new taxa were also isolated. The highest relative proportion of isolates able to degrade starch was found in compost SC (>70%), to degrade gelatine in compost DC (>70%), to degrade Tween 80 in compost PC (>90%), and to degrade poly-epsilon-caprolactones in compost DC (>80%). Compost MC presented the lowest relative proportions of isolates able to degrade starch (<25%), gelatine (<20%), and poly-epsilon-caprolactone (<40%). When compared with the others, the homemade composts presented higher relative proportions of Gram-positive isolates able to inhibit the target organisms Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, or Pseudomonas aeruginosa. In compost MC, none of the Gram-positive isolates was able to inhibit those targets.
References
Dees PM, Ghiorse WC (2001) Microbial diversity in hot synthetic compost as revealed by PCR-amplified rRNA sequences from cultivated isolates and extracted DNA. FEMS Microbiol Ecol 35:207–216
Epstein E (1997) The Science of Composting. Technomic Publication, Lancaster, PA, ISBN 1-56676-478-5
Fang M, Wong JMC (2000) Changes in thermophilic bacteria population and diversity during composting of coal fly ash and sewage sludge. Water Air Soil Pollut 124:333–343
Ferreira da Silva M, Tiago I, Veríssimo A, Boaventura AR, Nunes OC, Manaia CM (2006) Antibiotic resistance of enterococci and related bacteria in an urban wastewater treatment plant. FEMS Microbiol Ecol 55:322–329
Ferreira da Silva M, Vaz-Moreira I, Gonzalez-Pajuelo M, Nunes OC, Manaia CM (2007) Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant. FEMS Microbiol Ecol 60:166–176
Fracchia L, Dohrmann AB, Martinotti MG, Tebbe CC (2006) Bacterial diversity in finished compost and vermicompost: differences revealed by cultivation-independent analyses of PCR-amplified 16S rRNA genes. Appl Microbiol Biotechnol 71:942–952
Haruta S, Nakayama T, Nakamura K, Hemmi H, Ishii M, Igarashi Y, Nishino T (2005) Microbial diversity in biodegradation and reutilization processes of garbage (review). J Biosc Bioeng 99(1):1–11
Hassen A, Belguith K, Jedidi N, Cherif A, Cherif M, Boudabous A (2001) Microbial characterization during composting of municipal solid waste. Bioresour Technol 80:217–225
Lasaridi K, Protopapa I, Kotsou M, Pilidis G, Manios T, Kyriacou A (2006) Quality assessment of composts in the Greek market: the need for standards and quality assurance. J Environ Manag 80(1):56–65
Pietronave S, Fracchia L, Rinaldi M, Martinotti MG (2004) Influence of biotic and abiotic factors on human pathogens in a finished compost. Water Res 38:1963–1970
Silva E, Vaz-Moreira I, Silva C, Sarabando H, Nunes OC, Manaia CM, Cunha-Queda C (2006) A contribution for a quality study of several composts produced in Portugal—ORBIT 2006 biological waste management: from local to global. 5th International Conference, Weimar, Germany
Strom PF (1985) Identification of thermophilic bacteria in solid-waste composting. Appl Environ Microbiol 50:906–913
Takaku H, Kodaira S, Kimoto A, Nashimoto M, Takagi M (2006) Microbial communities in the garbage composting with rice hull as an amendment revealed by culture-dependent and independent approaches. J Biosci Bioeng 101:42–50
Tiago I, Teixeira I, Silva S, Chung P, Veríssimo A, Manaia CM (2004) Metabolic and genetic diversity of mesophilic and thermophilic bacteria isolated from composted municipal sludge on poly-ɛ-caprolactones. Curr Microbiol 49:407–414
Tiquia SM (2002) Evolution of extracellular enzyme activities during manure composting. J Appl Microbiol 92(4):764–775
Vaz-Moreira I, Nobre MF, Nunes OC, Manaia CM (2007) Pseudosphingobacterium domesticum gen. nov., sp. nov., isolated from home-made compost. Int J Syst Evol Microbiol 57:1535–1538
Vaz-Moreira I, Faria C, Nobre MF, Schumann P, Nunes OC, Manaia CM (2007) Paenibacillus humicus, sp. nov., isolated from chicken poultry litter compost. Int J Syst Evol Microbiol (in press)
Versalovic K, Koeuth T, Lupski JR (1991) Distribution of repetitive DNA sequences in Eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19(24):6823–6831
Wei Y, Fan Y, Wang M, Wang J (2000) Composting and compost application in China. Resour Conserv Recycl 30:277–300
Yilmaz M, Soran H, Beyatli Y (2006) Antimicrobial activities of some Bacillus spp. strains isolated from the soil. Microbiol Res 161:127–131
Acknowledgements
The authors gratefully acknowledge Prof. Margarida Silva and Engr. Conceição Almeida of the Grupo de Estudos Ambientais of Escola Superior de Biotecnologia for supplying the homemade composts.
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Vaz-Moreira, I., Silva, M.E., Manaia, C.M. et al. Diversity of Bacterial Isolates from Commercial and Homemade Composts. Microb Ecol 55, 714–722 (2008). https://doi.org/10.1007/s00248-007-9314-2
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DOI: https://doi.org/10.1007/s00248-007-9314-2