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Isolation and Characterization of Diuron-degrading Bacteria from Lotic Surface Water

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Abstract

The bacterial community structure of a diuron-degrading enrichment culture from lotic surface water samples was analyzed and the diuron-degrading strains were selected using a series of techniques combining temporal temperature gradient gel electrophoresis (TTGE) of 16 S rDNA gene V1–V3 variable regions, isolation of strains on agar plates, colony hybridization methods, and biodegradation assays. The TTGE fingerprints revealed that diuron had a strong impact on bacterial community structure and highlighted both diuron-sensitive and diuron-adapted bacterial strains. Two bacterial strains, designated IB78 and IB93 and identified as belonging to Pseudomonas sp. and Stenotrophomonas sp., were isolated and shown to degrade diuron in pure resting cells in a first-order kinetic reaction during the first 24 h of incubation with no 3,4-DCA detected. The percentages of degradation varied from 25% to 60% for IB78 and 20% to 65% for IB93 and for a diuron concentration range from 20 mg/L to 2 mg/L, respectively. It is interesting to note that diuron was less degraded by single isolates than by mixed resting cells, thereby underlining a cumulative effect between these two strains. To the best of our knowledge, this is the first report of diuron-degrading strains isolated from lotic surface water.

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References

  1. Altschul, SF, Madden, TL, Schaffer, AA, Zhang, J, Zhang, Z, Miller, W, Lipman, DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402

    Article  PubMed  CAS  Google Scholar 

  2. Četkauskaité, A, Grigonis, U, Berinskiené, J (1998) Biodegradation: selection of suitable model. Ecotoxicol Environ Saf 40: 19–28

    Article  PubMed  Google Scholar 

  3. Cullington, JE, Walker, A (1999) Rapid biodegradation of diuron and other phenylurea herbicides by a soil bacterium. Soil Biol Biochem 31: 677–686

    Article  CAS  Google Scholar 

  4. Dejonghe, W, Berteloot, E, Goris, J, Boon, N, Crul, K, Maertens, S, Höfte, M, De Vos, P, Verstraete, W, Top, EM (2003) Synergistic degradation of linuron by a bacterial consortium and isolation of a single linuron-degrading Variovorax strain. Appl Environ Microbiol 69: 1532–1541

    Article  PubMed  CAS  Google Scholar 

  5. El Deeb, BA, Soltan, SM, Ali, AM, Ali, KA (2000) Detoxification of the herbicide diuron by Pseudomonas sp. Folia Microbiol 45: 211–216

    Google Scholar 

  6. El-Dib, MA, Abou-Waly, HF (1998) Biodegradation of some triazines and phenylureas in surface waters. Water Res 32: 1881–1887

    Article  CAS  Google Scholar 

  7. El Fantroussi, S, Verschuere, L, Verstraete, W, Top, EM (1999) Effect of phenylurea herbicides on soil microbial communities estimated by analysis of 16SrRNA gene fingerprints and community-level physiological profiles. Appl Environ Microbiol 65: 982–988

    PubMed  Google Scholar 

  8. El Fantroussi, S (2000) Enrichment and molecular characterization of a bacterial culture that degrades Methoxy-Methyl urea herbicides and their aniline derivatives. Appl Environ Microbiol 66: 5110–5115

    Article  PubMed  Google Scholar 

  9. Ellis, PA, Camper, ND (1982) Aerobic degradation of diuron by aquatic microorganisms. J Environ Sci Health B 17: 277–289

    Article  PubMed  CAS  Google Scholar 

  10. El Sebai, T, Lagacherie, B, Soulas, G, Martin-Laurent, F (2004) Isolation and characterisation of an isoproturon-mineralising Methylopila sp. TES from French agricultural soil. FEMS Microbiol Lett 239: 103–110

    Article  PubMed  CAS  Google Scholar 

  11. Esposito, E, Paulillo, SM, Manfio, GP (1998) Biodegradation of the herbicide diuron in soil by indigenous actinomycetes. Chemosphere 37: 541–548

    Article  PubMed  CAS  Google Scholar 

  12. Field, JA, Reed, RL, Sawyer, TE, Griffith, SM, Wigington, PJ (2003) Diuron occurrence and distribution in soil and surface and ground water associated with grass seed production. J Environ Qual 32: 171–179

    Article  PubMed  CAS  Google Scholar 

  13. Geissbuhler, H (1973) The substituted ureas. In: Kearney, PC, Kaufman, DD (Eds.) Degradation of Herbicides, Marcel Dekker, New York, pp 79–111

    Google Scholar 

  14. Giacomazzi, S, Cochet, N (2004) Environmental impact of diuron transformation: a review. Chemosphere 56: 1021–1032

    Article  PubMed  CAS  Google Scholar 

  15. Heikinheimo, A, Lindström, M, Korkeala, H (2004) Enumeration and isolation of cpe-positive Clostridium perfringens spores from feces. J Clin Microbiol 42: 3992–3997

    Article  PubMed  CAS  Google Scholar 

  16. Hill, GD, MacGahen, JW, Baker, HM, Finnerty, DW, Bingeman, CW (1955) The fate of substituted urea herbicides in agricultural soils. Agron J 47: 93–104

    Article  CAS  Google Scholar 

  17. IFEN (2004) Etudes et travaux no. 42 (Juillet 2004): les pesticides dans les eaux—Sixième bilan annuel—Données 2002

  18. Joynt, J, Bischoff, M, Turco, R, Konopka, A, Nakatsu, CH (2006) Microbial community analysis of soils contaminated with lead, chromium and petroleum hydrocarbons. Microb Ecol 51: 209–219

    Article  PubMed  CAS  Google Scholar 

  19. Khadrani, A, Seigle-Murandi, F, Steiman, R, Vroumsia, T (1999) Degradation of three phenylurea herbicides (chlortoluron, isoproturon and diuron) by micromycetes isolated from soil. Chemosphere 38: 3041–3050

    Article  PubMed  CAS  Google Scholar 

  20. Kopczynski, ED, Bateson, MM, Ward, DM (1994) Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms. Appl Environ Microbiol 60: 746–748

    PubMed  CAS  Google Scholar 

  21. Landry, D, Dousset, S, Andreux, F (2006) Leaching of oryzalin and diuron through undisturbed vineyard soil columns under outdoor conditions. Chemosphere 62: 1736–1747

    Article  PubMed  CAS  Google Scholar 

  22. Lane, DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt, E, Goodfellow, M (Eds.) Nucleic Acid Techniques in Bacterial Systematics, John Wiley & Sons, Inc., New York, NY, pp 115–148

    Google Scholar 

  23. Leu, C, Singer, H, Stamm, C, Muller, SR, Schwarzenbach, RP (2004) Simultaneous assessment of sources, processes, and factors influencing herbicide losses to surface waters in a small agricultural catchment. Environ Sci Technol 38: 3827–3834

    Article  PubMed  CAS  Google Scholar 

  24. Leu, C, Singer, H, Stamm, C, Muller, SR, Schwarzenbach, RP (2004) Variability of herbicide losses from 13 fields to surface water within a small catchment after a controlled herbicide application. Environ Sci Technol 38: 3835–3841

    Article  PubMed  CAS  Google Scholar 

  25. Ludwig, W, Strunk, O, Westram, R, Richter, L, Meier, H, Yadhukumar, Buchner, A, Lai, T, Steppi, S, Jobb, G, Forster, W, Brettske, I, Gerber, S, Ginhart, AW, Gross, O, Grumann, S, Hermann, S, Jost, R, Konig, A, Liss, T, Lussmann, R, May, M, Nonhoff, B, Reichel, B, Strehlow, R, Stamatakis, A, Stuckmann, N, Vilbig, A, Lenke, M, Ludwig, T, Bode, A, Schleifer, KH (2004) ARB: a software environment for sequence data. Nucleic Acids Res 32: 1363–1371

    Article  PubMed  CAS  Google Scholar 

  26. Maidak, BL, Cole, JR, Parker, CT, Jr, Garrity, GM, Larsen, N, Li, B, Lilburn, TG, McCaughey, MJ, Olsen, GJ, Overbeek, R, Pramanik, S, Schmidt, TM, Tiedje, JM, Woese, CR (1999) A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27: 171–173

    Article  PubMed  CAS  Google Scholar 

  27. Manzo, S, Buono, S, Cremisini, C (2006) Toxic effect of irgarol and diuron on sea urchin Paracentrotus lividus early development, fertilization, and offspring quality. Arch Environ Contam Toxicol 51: 61–68

    Article  PubMed  CAS  Google Scholar 

  28. Muyzer, G, de Waal, EC, Uitterlinden, AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16 S rRNA. Appl Environ Microbiol 59: 695–700

    PubMed  CAS  Google Scholar 

  29. Pesce, S, Fajon, C, Bardot, C, Bonnemoy, F, Portelli, C, Bohatier, J (2006) Effects of the phenylurea herbicide diuron on natural riverine microbial communities in an experimental study. Aquat Toxicol 78: 303–314

    Article  PubMed  CAS  Google Scholar 

  30. Phyt’eauvergne (2004) Rapport d’activités du groupe régional d’action contre la pollution des eaux naturelles par les produits phytosanitaires, année 2003. Phyt’eauvergne, France

  31. Prichard, T, Troiano, J, Marade, J, Guo, F, Canevari, M (2005) Movement of diuron and hexazinone in clay soil and infiltrated pond water. J Environ Qual 34: 2005–2017

    Article  PubMed  CAS  Google Scholar 

  32. Ramwell, CT, Heather, AI, Shepherd, J (2002) Herbicide loss following application to a roadside. Pest Manag Sci 58: 695–701

    Article  PubMed  CAS  Google Scholar 

  33. Roberts, SJ, Walker, A, Cox, L, Weich, SJ (1998) Isolation of isoproturon-degrading bacteria from treated soil via three different routes. J Appl Microbiol 85: 309–316

    Article  PubMed  CAS  Google Scholar 

  34. Rousseaux, S, Hartmann, A, Soulas, G (2001) Isolation and characterisation of new Gram-negative and Gram-positive atrazine degrading bacteria from different French soils. FEMS Microbiol Ecol 36: 211–222

    Article  PubMed  CAS  Google Scholar 

  35. Rupp, DE, Peachey, RE, Warren, KL, Selker, JS (2006) Diuron in surface runoff and tile drainage from two grass-seed fields. J Environ Qual 35: 303–311

    Article  PubMed  CAS  Google Scholar 

  36. Shelton, DR, Khader, S, Karns, JS, Pogell, BM (1996) Metabolism of twelve herbicides by Streptomyces. Biodegradation 7: 129–136

    Article  PubMed  CAS  Google Scholar 

  37. Snellinx, Z, Taghavi, S, Vangronsveld, J, van der Lelie, D (2003) Microbial consortia that degrade 2,4-DNT by interspecies metabolism: isolation and characterisation. Biodegradation 14: 19–29

    Article  PubMed  CAS  Google Scholar 

  38. Sobieszczanski, J, Rodziewicz, A, Stempniewicz, R (1981) Microbiological transformation of herbicides in catabolic processes. Part II: the effect of diuron on the respiration activity of selected bacterial strains. Rocz Glebozn 32: 105–113

    CAS  Google Scholar 

  39. Sørensen, SR, Ronen, Z, Aamand, J (2001) Isolation from agricultural soil and characterization of a Sphingomonas sp. able to mineralize the phenylurea herbicide isoproturon. Appl Environ Microbiol 67: 5403–5409

    Article  PubMed  CAS  Google Scholar 

  40. Sørensen, SR, Bending, GD, Jacobsen, CS, Walker, A, Aamand, J (2003) Microbial degradation of isoproturon and related phenylurea herbicides in and below agricultural fields. FEMS Microbiol Ecol 45: 1–11

    Article  CAS  PubMed  Google Scholar 

  41. Sumpono, Perotti, P, Belan, A, Forestier, C, Lavedrine, B, Bohatier, J (2003) Effect of diuron on aquatic bacteria in laboratory-scale wastewater treatment ponds with special reference to Aeromonas species studied by colony hybridization. Chemosphere 50: 445–455

    Article  PubMed  CAS  Google Scholar 

  42. Tillmanns, GM, Wallnöffer, PR, Engelhardt, G, Olie, K, Huntzinger, O (1978) Oxidative dealkylation of five phenylurea herbicides by the fungus Cunninghamella echinulata Thaxter. Chemosphere 1: 59–74

    Article  Google Scholar 

  43. Tixier, C, Bogaerts, P, Sancelme, M, Bonnemoy, F, Twagilimana, L, Cuer, A, Bohatier, J, Veschambre, H (2000) Fungal biodegradation of a phenylurea herbicide, diuron: structure and toxicity of metabolites. Pest Manag Sci 56: 455–462

    Article  CAS  Google Scholar 

  44. Tixier, C, Sancelme, M, Bonnemoy, F, Cuer, A, Veschambre, H (2001) Degradation products of a phenylurea herbicide, diuron: synthesis, ecotoxicology, and biotransformation. Environ Toxicol Chem 20: 1381–1389

    Article  PubMed  CAS  Google Scholar 

  45. Tixier, C, Sancelme, M, Ait-Aissa, S, Widehem, P, Bonnemoy, F, Cuer, A, Truffaut, N, Veschambre, H (2002) Biotransformation of phenylurea herbicides by a soil bacterial strain, Arthrobacter sp. N2: structure, ecotoxicity and fate of diuron metabolite with soil fungi. Chemosphere 46: 519–526

    Article  PubMed  CAS  Google Scholar 

  46. Tomlin, C (Ed.) (1994) The pesticide manual. 10th ed. British Crop Protection Council. Farnham. Surrey. United Kingdom

  47. Turnbull, GA, Cullington, JE, Walker, A, Morgan, JAW (2001) Identification and characterisation of a diuron-degrading bacterium. Biol Fertil Soils 33: 472–476

    Article  CAS  Google Scholar 

  48. Turnbull, GA, Ousley, M, Walker, A, Shaw, E, Morgan, JAW (2001) Degradation of substituted phenylurea herbicides by Arthrobacter globiformis strain D47 and characterization of a plasmid-associated hydrolase gene, puhA. Appl Environ Microbiol 67: 2270–2275

    Article  PubMed  CAS  Google Scholar 

  49. Vianello, M, Vischetti, C, Scarponi, L, Zanin, G (2005) Herbicide losses in runoff events from a field with a low slope: role of a vegetative filter strip. Chemosphere 61: 717–725

    Article  PubMed  CAS  Google Scholar 

  50. Vroumsia, T, Steiman, R, Seigle-Murandi, F, Benoit-Guyod, JL, Khadrani, A (1996) Biodegradation of three substituted phenylurea herbicides (chlortoluron, diuron, and isoproturon) by soil fungi. A comparative study. Chemosphere 33: 2045–2056

    Article  PubMed  CAS  Google Scholar 

  51. Weisburg, WG, Barns, SM, Pelletier, DA, Lane, DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173: 697–703

    PubMed  CAS  Google Scholar 

  52. Widehem, P, Aït-Aïssa, S, Tixier, C, Sancelme, M, Veschambre, H, Truffaut, N (2002) Isolation, characterization and diuron transformation capacities of a bacterial strain Arthrobacter sp. N2. Chemosphere 46: 527–534

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Clarisse Mallet for carefully proofreading the manuscript. Special thanks to Anne-Catherine Lehours for advice on the use of DNA sequence analysis tools.

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  1. Laboratoire de Biologie des Protistes, UMR CNRS 6023, Université Blaise Pascal, Aubière cedex, 63177, France

    Isabelle Batisson, Stéphane Pesce & Jacques Bohatier

  2. Laboratoire de Synthèse Et Etude de Systèmes à Intérêt Biologiques, UMR 6504 CNRS, Université Blaise Pascal, Aubière cedex, 63177, France

    Pascale Besse-Hoggan & Martine Sancelme

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  1. Isabelle Batisson
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  2. Stéphane Pesce
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Correspondence to Isabelle Batisson.

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Batisson, I., Pesce, S., Besse-Hoggan, P. et al. Isolation and Characterization of Diuron-degrading Bacteria from Lotic Surface Water. Microb Ecol 54, 761–770 (2007). https://doi.org/10.1007/s00248-007-9241-2

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