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Pseudomonas fluorescens SBW25 Biofilm and Planktonic Cells Have Differentiable Raman Spectral Profiles

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Abstract

Biofilms, and other bacterial aggregations, are of significance in both environmental microbiology and in plant and human pathogenesis. Comparative single-cell Raman spectral analysis can differentiate between planktonic bacteria and those recovered from biofilms and appears to offer a new means by which to investigate bacterial cell physiology, metabolic status, and stress under different environmental conditions.

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Acknowledgements

We are grateful to Andrew Knowles and Simon FitzGerald from HORIBA Jobin-Yvon Ltd. UK for supplying the Raman Confocal Microscope.

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Authors and Affiliations

  1. Molecular Microbiology Ecology and Environmental Biotechnology Sections, CEH-Oxford, Mansfield Road, Oxford, OX1 3SR, UK

    Wei E. Huang, Susanne Ude & Andrew J. Spiers

  2. Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK

    Wei E. Huang, Susanne Ude & Andrew J. Spiers

Authors
  1. Wei E. Huang
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  2. Susanne Ude
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  3. Andrew J. Spiers
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Correspondence to Andrew J. Spiers.

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Huang, W.E., Ude, S. & Spiers, A.J. Pseudomonas fluorescens SBW25 Biofilm and Planktonic Cells Have Differentiable Raman Spectral Profiles. Microb Ecol 53, 471–474 (2007). https://doi.org/10.1007/s00248-006-9190-1

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  • DOI: https://doi.org/10.1007/s00248-006-9190-1

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