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Relationship Between Testate Amoeba (Protist) Communities and Atmospheric Heavy Metals Accumulated in Barbula indica (Bryophyta) in Vietnam

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Abstract

We studied the relationships between testate amoeba communities and heavy metal (Pb, Cd, Zn, Ni, Cu, Mn, and Fe) concentrations in the moss Barbula indica sampled at 29 sites in and around the city of Hanoi (Vietnam). Our first approach was to compare the heavy metal concentrations and testate amoeba variables between the city (zone 1) and the surrounding (zone 2). Mean moss concentrations of Pb, Cd, Zn, Ni, and Cu were significantly higher and testate amoeba species richness and abundance were significantly lower in zone 1 and the abundance of eight taxa differed significantly between the two zones. We then studied the correlation between heavy metals and testate amoebae. Species richness and abundance were correlated negatively to Pb concentration. Shannon H′ was negatively correlated to both Pb and Cd. The abundance of several species was negatively correlated with Pb, Cd, Zn, and Ni; however, at the community level, Pb emerged as the only significant variable in a redundancy analysis. Our results suggest that testate amoebae are sensitive to and may be good bioindicators for heavy metal pollution, especially lead. Further research is needed to understand the causal relationships underlying the observed patterns.

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Acknowledgments

The authors thank Nadia Crini for her assistance in chemical analyses; Renaud Scheifler and Michael Coeurdassier for helpful discussion throughout the work; Florence Mazier for initiating the first author into multivariate analysis; Quynh Trang Bui and Ngoc Nam Le for their help in moss sampling; Dr. Ninh Tran (Department of Biology, Hanoi National University, Vietnam) for identifying Bryophytes in Hanoi; the faculty of Agro-Biology (Hanoi University of Education, Vietnam) and the Centre for Mangrove Ecosystem Research (Hanoi National University, Vietnam) for providing punctual access to laboratory materials; Van Hoan Kieu (Faculty of Geology, Hanoi University of Education, Vietnam) for providing a map of Hanoi; and Prof. David Wilkinson (Biological and Earth Sciences, Liverpool John Moores University) and two anonymous reviewers for helpful comments on an earlier version of the manuscript. This work was partly supported by the French Embassy in Vietnam and EU project RECIPE (European Commission project n° EVK2-2002-00269 and Swiss State Secretariat for Education and Research SER project n° 01.0438-1).

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Authors and Affiliations

  1. Laboratoire de Biologie Environnementale, USC INRA, EA 3184, Université de Franche-Comté, Place Leclerc, 25030, Besançon, France

    H. Nguyen-Viet, N. Bernard, J. Cortet, P.-M. Badot & D. Gilbert

  2. École Polytechnique Fédérale de Lausanne (EPFL), Laboratoire des Systèmes Écologiques, Station 2, 1015, Lausanne, Switzerland

    E. A. D. Mitchell

  3. Institut Fédéral de Recherches WSL, Antenne Romande, Station 2, 1015, Lausanne, Switzerland

    E. A. D. Mitchell

  4. Institut Méditerranéen d’Ecologie et Paléoécologie, UMR CNRS 6116, Faculté Saint Jérôme, case 462, 13397, Marseilles cedex 20, France

    J. Cortet

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  1. H. Nguyen-Viet
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  2. N. Bernard
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  3. E. A. D. Mitchell
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Correspondence to H. Nguyen-Viet.

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Nguyen-Viet, H., Bernard, N., Mitchell, E.A.D. et al. Relationship Between Testate Amoeba (Protist) Communities and Atmospheric Heavy Metals Accumulated in Barbula indica (Bryophyta) in Vietnam. Microb Ecol 53, 53–65 (2007). https://doi.org/10.1007/s00248-006-9108-y

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