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Evaluation of Remediation of Coal Mining Wastewater by Chitosan Microspheres Using Biomarkers

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Abstract

Acidic mine waters have a marked influence on the surrounding environment and pose a serious threat through long-term environmental degradation. Therefore, it is important to improve and monitor water quality with the aim of decreasing the hazard presented by this effluent emission. The aim of this work was to evaluate the remediation of mining wastewater effluents by chitosan microspheres using biomarkers of exposure and effect. DNA damage (Comet assay) and several biomarkers of oxidative stress, such as lipoperoxidation levels (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities, and contents of reduced glutathione (GSH), were measured in blood and liver of tilapia (Oreochromis niloticus) exposed for 7, 15, and 30 days to dechlorinated tap water, 10% coal mining wastewater (CMW), and coal mining wastewater treated with chitosan microspheres (RCM). The results indicate that hepatic TBARS levels were significantly higher in fish exposed to CMW after 7, 15, and 30 days (100%, 86%, and 63%, respectively), and after remediation there was no significant difference in relation to the control group. Hepatic GSH concentrations were lower than control values for CMW after 7 and 15 days of exposure (34% decrease at both times), and this concentration was normalized by treatment with chitosan. SOD showed increased activity in liver after 15 and 30 days of exposure, 30% and 36%, respectively, and in fish exposed to RCM there was no change in this activity compared with the control group. Increased CAT activity in liver was observed during all experimental periods in fish exposed to CMW (46%, 50%, and 56% at 7, 15, and 30 days, respectively) compared with the control or treated-water groups. The highest increase in hepatic GST activity (106%) was observed only in fish exposed to CMW for 30 days. There was an increase in DNA damage in liver (50% at 7 and 15 days) and blood (79%, 77%, and 48% at 7, 15, and 30 days, respectively) after exposure to CMW. In contrast, the fish exposed to wastewater treated with chitosan microspheres exhibited DNA fragmentation indexes similar to the control group. The results obtained indicate the use of oxidative stress biomarkers as useful tools for the toxicity evaluation of coal mining effluents and also suggest that chitosan microspheres may be used as an alternative approach for remediation of coal mining wastewaters.

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Acknowledgments

The investigators thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico for financial support and fellowships.

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Authors and Affiliations

  1. Departamento de Bioquímica, Universidade Federal de Santa Catarina, Campus Universitário da Trindade, 88040-900, Florianópolis, Santa Catarina, Brazil

    J. C. Benassi, P. L. Lima & R. C. Pedrosa

  2. Departamento de Química, Universidade Federal de Santa Catarina, Santa Catarina, Brazil

    R. Laus, M. C. M. Laranjeira & V. T. Fávere

  3. Departamento de Ecologia e Zoologia, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil

    D. Wilhelm-Filho

  4. Departamento de Farmácia, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil

    R. Geremias

  5. Departamento de Engenharia Ambiental/IPAT, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil

    C. T. B. Menezes

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  1. J. C. Benassi
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  2. R. Laus
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  6. M. C. M. Laranjeira
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  8. V. T. Fávere
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  9. R. C. Pedrosa
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Correspondence to R. C. Pedrosa.

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Benassi, J.C., Laus, R., Geremias, R. et al. Evaluation of Remediation of Coal Mining Wastewater by Chitosan Microspheres Using Biomarkers. Arch Environ Contam Toxicol 51, 633–640 (2006). https://doi.org/10.1007/s00244-005-0187-4

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  • DOI: https://doi.org/10.1007/s00244-005-0187-4

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