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A prospective case–control study on the evaluation of oxidative stress in renal stone formers

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Abstract

Association of increased oxidative stress (OS) with the pathophysiology of renal stone formation has not been explored greatly in the field of urolithiasis. In this prospective case–control study, we measured 24-h urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG) levels in patients with urolithiasis and compared them with matched healthy controls. We also measured 24-h urinary uric acid, calcium, oxalate, and citrate levels in patients with renal stone disease and studied their relation with urinary 8-OHdG levels. Seventy-five cases of renal stone disease and 75 well-matched controls were included. Median 24-h urinary 8-OHdG levels were significantly higher in cases compared to controls (7.6 vs. 3.7 µg/g of creatinine; p < 0.000). Receiver-operating curve (ROC) analysis for 8-OHdG between cases and controls revealed an area under the curve of 0.90. At 8-OHdG (µg/g of creatinine) value of 5 or more, a sensitivity and a specificity of 84% each were obtained. A positive correlation between 8-OHdG (µg/g of creatinine) and 24-h urinary oxalate level was noted (r = 0.461, p = 0.000). No correlation between 8-OHdG (µg/g of creatinine) and other variables was noted. On multivariate linear regression analysis, we noted 24-h urinary oxalate levels to be an independent predictor of urinary 8-OHdG levels. OS is significantly higher in patients with renal stone diseases compared to healthy controls. Urinary oxalate levels were significantly correlated with urinary 8-OHdG levels.

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The basic study data of the current study will be made available to the journal only after proper consent from the Institute, where the study was conducted.

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Funding

This study was financially supported by Post Graduate Institute of Medical Education and Research, Chandigarh, India.

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Authors and Affiliations

  1. Department of Urology, PGIMER, Chandigarh, India

    V. Manoharan, G. Sharma & S. K. Devana

  2. Department of Biochemistry, PGIMER, Chandigarh, India

    S. Sharma

  3. Department of Biophysics, PGIMER, Chandigarh, India

    P. Avti

  4. Department of General Surgery, PGIMER, Chandigarh, India

    S. Chandramouli

Authors
  1. V. Manoharan
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  2. G. Sharma
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  3. S. K. Devana
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  4. S. Sharma
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  5. P. Avti
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  6. S. Chandramouli
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Contributions

Manoharan V involved in protocol development, data collection and management, data analysis, and manuscript writing. Sharma G involved in data collection and management, data analysis, and manuscript writing. Devana SK involved in data collection and management, data analysis, and manuscript writing. Sharma S involved in data collection and management and manuscript editing. Avti P involved in manuscript writing and editing. Sivaraman C involved in data collection and management and manuscript editing.

Corresponding author

Correspondence to S. K. Devana.

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The authors declare that they have no conflict of interest.

Statement of human rights

This research involved human participants, and the study protocol was approved by the institute’s ethics committee (Ref: INT/IEC/2017/1396). We certify that the study was performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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This article does not contain any studies with animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Manoharan, V., Sharma, G., Devana, S.K. et al. A prospective case–control study on the evaluation of oxidative stress in renal stone formers. Urolithiasis 52, 18 (2024). https://doi.org/10.1007/s00240-023-01514-7

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