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Search for Novel Plasma Membrane Proteins as Potential Biomarkers in Human Mesenchymal Stem Cells Derived from Dental Pulp, Adipose Tissue, Bone Marrow, and Hair Follicle

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Abstract

One of the drawbacks preventing the use of mesenchymal stem cells (MSCs) in clinical practice is the heterogeneous nature of their cultures. MSC cultures are not homogeneously formed by the MSCs and may contain non-mesenchymal cell types. Therefore, prior to use in clinics or research, complete characterization of MSCs should be performed to demonstrate the existence or absence of proper stem cell markers, many of which are happened to be cell-surface proteins. Unfortunately, the success of MSC characterization studies is limited due to the low specificity of the currently available cell-surface markers. Therefore, in this study, we aimed to investigate the plasma membrane (PM) proteins of MSCs isolated from human dental pulp (DP), adipose tissue (AT), bone marrow (BM), and hair follicle (HF) with the hope of proposing novel putative specific MSC markers. Differential-velocity centrifugation was used to enrich PM proteins. The isolated proteins were then identified by nLC-MS/MS and subjected to bioinformatics analysis. Proteins that were unique to each MSC type (CD9, CD10, CD63 for DP-MSCs; CD26, CD81, CD201, CD364 for AT-MSCs; Cd49a, CD49d for HF-MSCs; CD49e, CD56, CD92, CD97, CD156b, CD156c, CD220, CD221, CD298, CD315 for BM-MSCs) and common to all four MSC types (CD13, CD29, CD44, CD51, CD59, CD73, CD90) were identified. Uncharacterized proteins that have transmembrane (TM) domains were also detected. Some of the proteins identified in this study were the putative cell-surface markers that might be used for characterization of MSCs.

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If acquired, the raw data for LC–MS/MS experiments will be provided via e-mail.

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Acknowledgements

This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) with the grant number of 113S868. The funding body had no role in the design or execution of the study.

Funding

This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) under the grant number of 113S868.

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Authors and Affiliations

  1. Department of Medical Biology, School of Medicine, Kocaeli University, 41001, Kocaeli, Turkey

    Gurler Akpinar & Murat Kasap

  2. Department of Biomedical Engineering, Technology Faculty, Kocaeli University, 41001, Kocaeli, Turkey

    Kubra Karaosmanoglu Yoneten

  3. Department of Histology and Embryology, School of Medicine, Istinye University, 34010, Istanbul, Turkey

    Erdal Karaoz

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  1. Gurler Akpinar
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  2. Kubra Karaosmanoglu Yoneten
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  3. Murat Kasap
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Contributions

GA: Experimental design, carrying out experiments, writing the manuscript. KKY: Experimental design, carrying out experiments. MK: Experimental design, carrying out experiments, writing the manuscript. EK: Stem cell isolation and characterization experiments.

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Correspondence to Murat Kasap.

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The MSC lines used in this study were previously isolated and used in several studies of ours. Their ethical approvals for isolation were granted by Kocaeli University Ethics Committee.

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Akpinar, G., Yoneten, K.K., Kasap, M. et al. Search for Novel Plasma Membrane Proteins as Potential Biomarkers in Human Mesenchymal Stem Cells Derived from Dental Pulp, Adipose Tissue, Bone Marrow, and Hair Follicle. J Membrane Biol 254, 409–422 (2021). https://doi.org/10.1007/s00232-021-00190-1

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