Abstract
Studies made in the Madin-Darby canine kidney (MDCK) epithelial cell line showed that ouabain regulates cell adhesion and cell-adhesion-related biological processes, such as migration. Here, we demonstrated that 10 nM ouabain accelerates collective cell migration and heals wounds in cultured MDCK cell monolayers. Ouabain-induced acceleration of cell migration depends on activation of the cSrc-ERK1/2 signaling cascade, as it was inhibited by the kinase inhibitors PP2 and PD98059. Activation of the cSrc-ERK1/2 signaling cascade increased expression and activation of the extracellular matrix metalloproteinase-2 (MMP-2). Inhibition of MMP activity using the generic inhibitor GM6001 or the potent iMMP-2 inhibitor prevented the accelerative effect of ouabain. Likewise, Focal Adhesion Kinase (FAK) inhibition with the transfection of dominant negative peptide FRNK impaired the effect of ouabain. These results suggest that ouabain binding to the Na+,K+-ATPase accelerates collective migration of MDCK cells through activation of the cSrc-ERK1/2-FAK signaling cascade and promoting secretion and MMP activity.
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06 July 2019
The original version of the article unfortunately contained an error in the author group. Dr. Isabel Larré was not submitted and published in the original version.
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Acknowledgements
This work was supported by National Research Council of Mexico to RCG (Conacyt P221513 and SEP-Cinvestav 143) and by the Faculty Diversity Scholars Award from the University of Massachusetts Medical School to TP-B. We thank the technical help of Mr. E. Estrada.
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The original version of the article was revised. Dr. I. Larré is included as fifth author in the author group.
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Verdejo-Torres, O., Flores-Maldonado, C., Padilla-Benavides, T. et al. Ouabain Accelerates Collective Cell Migration Through a cSrc and ERK1/2 Sensitive Metalloproteinase Activity. J Membrane Biol 252, 549–559 (2019). https://doi.org/10.1007/s00232-019-00066-5
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DOI: https://doi.org/10.1007/s00232-019-00066-5