Abstract
Human amniotic membrane (hAM) has recently drawn attention as an upcoming anti-cancer therapy. Regarding the strategies which have already investigated, little is known about hAM protein extracts (hAMPE) effect on cancer. So, this work aims to study the effect of hAMPE in metabolic activity of several human cancer cell lines. hAMPE were mechanically obtained, thus avoiding the effect of detergents and other reagents commonly used in protein extraction under the cell lines studied. After quantification of proteins in hAMPE, their effect on the metabolic activity of 21 human cancer cell lines was assessed by 3-(4,5-dimethylthia-zolyl-2)2,5-diphenyltetrazolium bromide (MTT) assay. Our results indicate that there is an inhibition of metabolic activity until 25 and 50 % in two and seven cell lines, respectively. Five cell lines proved to be very sensitive to hAMPE, being its metabolic activity more than 50 % inhibited. Our results show that hAMPE can inhibit the metabolic activity of some human cancer cell lines. However, research about this cell line-dependent response to hAMPE becomes indispensable.
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Acknowledgments
Ana Catarina Mamede (SFRH/BD/73649/2010), Mafalda Laranjo (SFRH/BD/44957/2008), Maria João Carvalho (SFRH/SINTD/60068/2009), Ana Salomé Pires (SFRH/BD/75300/2010) and Ana Filipa Brito (SFRH/BD/61378/2009) wishes to thank to Portuguese Foundation for Science and Technology for PhD Grants. The authors would like to thank to Dr. Rui Gradiz for the collaboration in this work. Finally, the authors thank to Obstetrics Service of the Coimbra Hospital and University Centre for the collection of human tissues used in this work.
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Mamede, A.C., Laranjo, M., Carvalho, M.J. et al. Effect of Amniotic Membrane Proteins in Human Cancer Cell Lines: An Exploratory Study. J Membrane Biol 247, 357–360 (2014). https://doi.org/10.1007/s00232-014-9642-3
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DOI: https://doi.org/10.1007/s00232-014-9642-3