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Expression of Transient Receptor Potential Vanilloid Channels TRPV5 and TRPV6 in Human Blood Lymphocytes and Jurkat Leukemia T Cells

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Abstract

Regulation of Ca2+ entry is a key process for lymphocyte activation, cytokine synthesis and proliferation. Several members of the transient receptor potential (TRP) channel family can contribute to changes in [Ca2+]in; however, the properties and expression levels of these channels in human lymphocytes continue to be elusive. Here, we established and compared the expression of the most Ca2+-selective members of the TRPs, Ca2+ channels transient receptor potential vanilloid 5 and 6 (TRPV5 and TRPV6), in human blood lymphocytes (HBLs) and leukemia Jurkat T cells. We found that TRPV6 and TRPV5 mRNAs are expressed in both Jurkat cells and quiescent HBLs; however, the levels of mRNAs were significantly higher in malignant cells than in quiescent lymphocytes. Western blot analysis showed TRPV5/V6 proteins in Jurkat T cells and TRPV5 protein in quiescent HBLs. However, the expression of TRPV6 protein was switched off in quiescent HBLs and turned on after mitogen stimulation of the cells with phytohemagglutinin. Inwardly directed monovalent currents that displayed characteristics of TRPV5/V6 currents were recorded in both Jurkat cells and normal HBLs. In outside–out patch-clamp studies, currents were reduced by ruthenium red, a nonspecific inhibitor of TRPV5/V6 channels. In addition, ruthenium red downregulated cell-cycle progression in both activated HBLs and Jurkat cells. Thus, we identified TRPV5 and TRPV6 calcium channels, which can be considered new candidates for Ca2+ entry into human lymphocytes. The correlation between expression of TRPV6 channels and the proliferative status of lymphocytes suggests that TRPV6 may be involved in the physiological and/or pathological proliferation of lymphocytes.

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Acknowledgements

The authors are grateful to Drs. Viktor Kharazia (Ernest Gallo Clinic and Research Center, University of California) and Nevin Mlodik (Medical College of Wisconsin) for critical reading and correcting of this article. This work was supported by grants from the Russian Foundation for Basic Research (11-04-01030) and the Molecular and Cell Biology Program of the Russian Academy of Sciences.

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 4 Tikhoretsky Ave., St. Petersburg, 194064, Russia

    Irina O. Vassilieva, Victor N. Tomilin, Irina I. Marakhova, Alla N. Shatrova, Yuri A. Negulyaev & Svetlana B. Semenova

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  1. Irina O. Vassilieva
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  2. Victor N. Tomilin
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  3. Irina I. Marakhova
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  4. Alla N. Shatrova
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  5. Yuri A. Negulyaev
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  6. Svetlana B. Semenova
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Correspondence to Svetlana B. Semenova.

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Vassilieva, I.O., Tomilin, V.N., Marakhova, I.I. et al. Expression of Transient Receptor Potential Vanilloid Channels TRPV5 and TRPV6 in Human Blood Lymphocytes and Jurkat Leukemia T Cells. J Membrane Biol 246, 131–140 (2013). https://doi.org/10.1007/s00232-012-9511-x

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  • DOI: https://doi.org/10.1007/s00232-012-9511-x

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