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Kinetic Analysis of Gill (Na+,K+)-ATPase Activity in Selected Ontogenetic Stages of the Amazon River Shrimp, Macrobrachium amazonicum (Decapoda, Palaemonidae): Interactions at ATP- and Cation-Binding Sites

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Abstract

We investigated modulation by ATP, Mg2+, Na+, K+ and NH4 + and inhibition by ouabain of (Na+,K+)-ATPase activity in microsomal homogenates of whole zoeae I and decapodid III (formerly zoea IX) and whole-body and gill homogenates of juvenile and adult Amazon River shrimps, Macrobrachium amazonicum. (Na+,K+)-ATPase-specific activity was increased twofold in decapodid III compared to zoea I, juveniles and adults, suggesting an important role in this ontogenetic stage. The apparent affinity for ATP (K M = 0.09 ± 0.01 mmol L−1) of the decapodid III (Na+,K+)-ATPase, about twofold greater than the other stages, further highlights this relevance. Modulation of (Na+,K+)-ATPase activity by K+ also revealed a threefold greater affinity for K+ (K 0.5 = 0.91 ± 0.04 mmol L−1) in decapodid III than in other stages; NH4 + had no modulatory effect. The affinity for Na+ (K 0.5 = 13.2 ± 0.6 mmol L−1) of zoea I (Na+,K+)-ATPase was fourfold less than other stages. Modulation by Na+, Mg2+ and NH4 + obeyed cooperative kinetics, while K+ modulation exhibited Michaelis-Menten behavior. Rates of maximal Mg2+ stimulation of ouabain-insensitive ATPase activity differed in each ontogenetic stage, suggesting that Mg2+-stimulated ATPases other than (Na+,K+)-ATPase are present. Ouabain inhibition suggests that, among the various ATPase activities present in the different stages, Na+-ATPase may be involved in the ontogeny of osmoregulation in larval M. amazonicum. The NH4 +-stimulated, ouabain-insensitive ATPase activity seen in zoea I and decapodid III may reflect a stage-specific means of ammonia excretion since functional gills are absent in the early larval stages.

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References

  • Anger K (2001) The biology of decapod crustacean larvae. Crustacean issues 14. Balkema, Lisse

  • Anger K (2003) Salinity as a key parameter in the larval biology of decapod crustaceans. Invertebr Reprod Dev 43:29–45

    Article  Google Scholar 

  • Anger K, Hayd L, Knott J, Nettelmann U (2009) Patterns of larval growth and chemical composition in the Amazon River prawn, Macrobrachium amazonicum. Aquaculture 287:341–348

    Article  CAS  Google Scholar 

  • Araujo MC, Valenti WC (2007) Feeding habit of the Amazon River prawn Macrobrachium amazonicum larvae. Aquaculture 265:187–193

    Article  Google Scholar 

  • Augusto A, Greene LJ, Laure HJ, McNamara JC (2007) The ontogeny of isosmotic intracellular regulation in the diadromous, freshwater palaemonid shrimps, Macrobrachium amazonicum and M. olfersi (Crustacea, Decapoda). J Crust Biol 27:626–634

    Article  Google Scholar 

  • Axelsen MG, Palmgren KB (1998) Evolution of P-type ATPases. Biochim Biophys Acta 1365:37–45

    Article  PubMed  Google Scholar 

  • Belli NM, Faleiros RO, Firmino KCS, Masui DC, Leone FA, McNamara JC, Furriel RPM (2009) Na,K-ATPase activity and epithelial interfaces in gills of the freshwater shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae). Comp Biochem Physiol A 152:431–439

    Article  CAS  Google Scholar 

  • Blanco G (2005) Na,K-ATPase subunit heterogeneity as a mechanism for tissue-specific ion regulation. Semin Nephrol 25:292–303

    Article  PubMed  CAS  Google Scholar 

  • Blanco G, Mercer RW (1998) Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function. Am J Physiol Renal Physiol 275:F633–F650

    CAS  Google Scholar 

  • Charmantier G (1998) Ontogeny of osmoregulation in crustaceans: a review. Invertebr Reprod Dev 33:177–190

    Article  CAS  Google Scholar 

  • Charmantier G, Anger K (2010) Ontogeny of osmoregulatory patterns in the South American shrimp Macrobrachium amazonicum: loss of hypo-regulation in a land-locked population indicates phylogenetic separation from estuarine ancestors. J Exp Mar Biol Ecol 396:89–98

    Article  Google Scholar 

  • Collart OO, Rabelo H (1996) Variation in egg size of the fresh-water prawn Macrobrachium amazonicum (Decapoda: Palaemonidae). J Crust Biol 16:684–688

    Article  Google Scholar 

  • Conte FP, Droukas PC, Ewing RD (1977) Development of sodium regulation and de novo synthesis of Na+,K+-activated ATPase in larval brine shrimp, Artemia salina. J Exp Zool 202:339–362

    Article  CAS  Google Scholar 

  • Crambert G, Hasler U, Beggah AT, Yu C, Modyanov NN, Horisberger JD, Lelievre L, Geering K (2000) Transport and pharmacological properties of nine different human Na,K-ATPase isozymes. J Biol Chem 275:1976–1986

    Article  PubMed  CAS  Google Scholar 

  • Escalante R, Garcia-Sáez A, Sastre L (1995) In situ hybridization analyses of Na,K-ATPase α-subunit expression during early larval development of Artemia franciscana. J Histochem Cytochem 43:391–399

    Article  PubMed  CAS  Google Scholar 

  • Faleiros RO, Goldman MH, Furriel RP, McNamara JC (2010) Differential adjustment in gill Na+/K+- and V-ATPase activities and transporter mRNA expression during osmoregulatory acclimation in the cinnamon shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae). J Exp Biol 213:3894–3905

    Article  PubMed  CAS  Google Scholar 

  • Faria SC, Augusto AS, McNamara JC (2011) Intra- and extracellular osmotic regulation in the hololimnetic Caridea and Anomura: a phylogenetic perspective on the conquest of fresh water by the decapod Crustacea. J Comp Physiol B 181:175–186

    Article  PubMed  Google Scholar 

  • Felder JM, Felder DL, Hand SC (1986) Ontogeny of osmoregulation in the estuarine ghost shrimp Callianassa jamaicense var. louisianensis Schmitt (Decapoda, Thalassinidea). J Exp Mar Biol Ecol 99:91–105

    Article  CAS  Google Scholar 

  • Fontes CFL, Barrabin H, Scofano HM, Norby JG (1992) The role of Mg2+ and K+ in the phosphorylation of Na+,K+-ATPase by ATP in the presence of dimethylsulfoxide but in the absence of Na+. Biochim Biophys Acta 1104:215–225

    Article  PubMed  CAS  Google Scholar 

  • Freire CA, Cavassin F, Rodrigues EN, Torres AH, McNamara JC (2003) Adaptive patterns of osmotic and ionic regulation, and the invasion of fresh water by the palaemonid shrimps. Comp Biochem Physiol A 136:771–778

    Article  Google Scholar 

  • Freire CA, Onken H, McNamara JC (2008) A structure-function analysis of ion transport in crustacean gills and excretory organs. Comp Biochem Physiol A 151:272–304

    Article  Google Scholar 

  • Furriel RPM, McNamara JC, Leone FA (2000) Characterization of (Na+,K+)-ATPase in gill microsomes of the freshwater shrimp Macrobrachium olfersi. Comp Biochem Physiol B 126:303–315

    Article  PubMed  CAS  Google Scholar 

  • Furriel RPM, Masui DC, McNamara JC, Leone FA (2004) Modulation of gill Na+,K+-ATPase activity by ammonium ions: putative coupling of nitrogen excretion and ion uptake in the freshwater shrimp Macrobrachium olfersii. J Exp Zool A 301:63–74

    Google Scholar 

  • Furriel RPM, Firmino KCS, Masui DC, Faleiros RO, Torres AH, McNamara JC (2010) Structural and biochemical correlates of Na+,K+-ATPase driven ion uptake across the posterior epithelium of the true freshwater crab, Dilocarcinus pagei (Brachyura, Trichodactylidae). J Exp Zool A 313:508–523

    Google Scholar 

  • Garçon DP, Masui DC, Mantelatto FLM, McNamara JC, Furriel RPM, Leone FA (2007) K+ and NH4 + modulate gill (Na+,K+)-ATPase activity in the blue crab, Callinectes ornatus: fine tuning of ammonia excretion. Comp Biochem Physiol A 147:145–155

    Article  Google Scholar 

  • Garçon DP, Masui DC, Mantelatto FLM, McNamara JC, Furriel RPM, Leone FA (2009) Hemolymph ionic regulation and adjustments in gill (Na+,K+)-ATPase activity during salinity acclimation in the swimming crab Callinectes ornatus (Decapoda, Brachyura). Comp Biochem Physiol A 154:44–55

    Article  Google Scholar 

  • Geering K (2008) Functional roles of Na-K-ATPase subunits. Curr Opin Nephrol Hypertens 17:526–532

    Article  PubMed  CAS  Google Scholar 

  • Genovese G, Luchetti CG, Luquet CM (2004) Na+/K+-ATPase activity and gill ultrastructure in the hyper-hypo-regulating crab Chasmagnathus granulatus acclimated to dilute, normal, and concentrated seawater. Mar Biol 144:111–118

    Article  CAS  Google Scholar 

  • Gonçalves RR, Masui DC, McNamara JC, Mantelatto FLM, Garçon DP, Furriel RPM, Leone FA (2006) A kinetic study of the gill (Na+,K+)-ATPase, and its role in ammonia excretion in the intertidal hermit crab, Clibanarius vittatus. Comp Biochem Physiol A 145:346–356

    Google Scholar 

  • Guest WC (1979) Laboratory life history of the palaemonid shrimp Macrobrachium amazonicum (Heller) (Decapoda, Palaemonidae). Crustaceana 37:141–152

    Article  Google Scholar 

  • Haond C, Bonnal L, Charmantier G, Trilles JP (1999) Ontogeny of intracellular isosmotic regulation in the European lobster Homarus gammarus. Physiol Biochem Zool 72:534–544

    Article  PubMed  CAS  Google Scholar 

  • Harris RR, Bayliss D (1988) Gill (Na+/K+)-ATPases in decapod crustaceans: distribution and characteristics in relation to Na+ regulation. Comp Biochem Physiol A 90:303–308

    Article  Google Scholar 

  • Holliday CW (1985) Salinity-induced changes in gill Na,K-ATPase activity in the mud fiddler crab Uca pugnax. J Exp Zool 233:199–208

    Article  CAS  Google Scholar 

  • Huong DTT, Jayasankar V, Jasmani S, Saido-Sakanaka H, Wigginton AJ, Wilder MN (2004) Na/K-ATPase activity during larval development in the giant freshwater prawn Macrobrachium rosenbergii and the effects of salinity on survival rates. Fish Sci 70:518–520

    Article  CAS  Google Scholar 

  • Ituarte RB, Mañanes AAL, Spivak ED, Anger K (2008) Activity of Na+,K+-ATPase in a freshwater shrimp, Palaemonetes argentinus (Caridea, Palaemonidae): ontogenetic and salinity-induced changes. Aquat Biol 3:283–290

    Article  Google Scholar 

  • Jorgensen PL, Pedersen PA (2001) Structure–function relationships of Na+,K+, ATP, or Mg2+ binding and energy transduction in Na+,K+-ATPase. Biochim Biophys Acta 1505:57–74

    Article  PubMed  CAS  Google Scholar 

  • Jorgensen PL, Hakansson KO, Karlish SJD (2003) Structure and mechanism of Na,K-ATPase: functional sites and their interactions. Annu Rev Physiol 65:817–849

    Article  PubMed  CAS  Google Scholar 

  • Kaplan JH (2002) Biochemistry of Na,K-ATPase. Annu Rev Biochem 30:511–533

    Article  Google Scholar 

  • Khodabandeh S, Charmantier G, Charmantier-Daures M (2006) Immunolocalization of Na,K-ATPase in osmoregulatory organs during the embryonic and post-embryonic development of the lobster Homarus gammarus. J Crust Biol 26:515–523

    Article  Google Scholar 

  • Kirschner LB (2004) The mechanism of sodium chloride uptake in hyperregulating aquatic animals. J Exp Biol 207:1439–1452

    Article  PubMed  CAS  Google Scholar 

  • Lee KJ, Watts SA (1994) Specific activity of Na+,K+ ATPase is not altered in response to changing salinities during early development of the brine shrimp, Artemia franciscana. Physiol Zool 67:910–924

    CAS  Google Scholar 

  • Leone FA, Baranauskas JA, Furriel RPM, Borin IA (2005a) SigrafW: an easy-to-use program for fitting enzyme kinetic data. Biochem Mol Biol Educ 33:399–403

    Article  PubMed  CAS  Google Scholar 

  • Leone FA, Furriel RPM, McNamara JC, Mantelatto FLM, Masui DC, Rezende LA, Gonçalves RR, Garçon D (2005b) (Na+,K+)-ATPase from crustacean gill microsomes: a molecular marker to evaluate adaptation to biotopes of different salinity. Trends Comp Biochem Physiol 11:1–15

    CAS  Google Scholar 

  • Lima AG, McNamara JC, Terra WR (1997) Regulation of hemolymph osmolytes and gill (Na+,K+)-ATPase activities during acclimation to saline media in the freshwater shrimp Macrobrachium olfersi (Wiegmann 1836) (Decapoda Palaemonidae). J Exp Mar Biol Ecol 215:81–91

    Article  CAS  Google Scholar 

  • Lucu C, Towle DW (2003) Na+,K+-ATPase in gills of aquatic crustacea. Comp Biochem Physiol A 135:195–214

    Article  Google Scholar 

  • Maciel CR, Valenti WC (2009) Biology, fisheries and aquaculture of the Amazon River prawn Macrobrachium amazonicum: a review. Nauplius 17:61–79

    Google Scholar 

  • Magalhães C (1985) Desenvolvimento larval obtido em laboratório de palemonídeos da Região Amazônica. I. Macrobrachium amazonicum (Heller, 1862) (Crustacea, Decapoda). Amazoniana 9:247–274

    Google Scholar 

  • Marks MJ, Seeds NW (1978) A heterogeneous ouabain-ATPase interaction in mouse brain. Life Sci 23:2735–2744

    Article  PubMed  CAS  Google Scholar 

  • Martin DW (2005) Structure–function relationships in the Na+,K+-pump. Semin Nephrol 25:282–291

    Article  PubMed  CAS  Google Scholar 

  • Masui DC, Furriel RPM, McNamara JC, Mantelatto FLM, Leone FA (2002) Modulation by ammonium ions of gill microsomal (Na+,K+)-ATPase in the swimming crab Callinectes danae: a possible mechanism for regulation of ammonia excretion. Comp Biochem Physiol C 132:471–482

    CAS  Google Scholar 

  • Masui DC, Furriel RPM, Silva ECC, Mantelatto FLM, McNamara JC, Barrabin H, Scofano HM, Fontes CFL, Leone FA (2005) Gill microsomal (Na+,K+)-ATPase from the blue crab Callinectes danae: interactions at cationic sites. Int J Biochem Cell Biol 37:2521–2535

    Article  PubMed  CAS  Google Scholar 

  • Masui DC, Silva ECC, Mantelatto FLM, McNamara JC, Barrabin H, Scofano HM, Fontes CFL, Furriel RPM, Leone FA (2008) The crustacean gill (Na+,K+)-ATPase: allosteric modulation of high- and low-affinity ATP-binding sites by sodium and potassium. Arch Biochem Biophys 479:139–144

    Article  PubMed  CAS  Google Scholar 

  • McNamara JC, Faria SC (2012) Evolution of osmoregulatory patterns and gill ion transport mechanisms in the decapod Crustacea: a review. J Comp Physiol B. doi:10.1007/s00360-012-0665-8

  • McNamara JC, Lima AG (1997) The route of ion water movements across the gill epithelium of the freshwater shrimp Macrobrachium olfersii (Decapoda, Palaemonidae): evidence from ultrastructural changes induced by acclimation to saline media. Biol Bull 192:321–331

    Article  Google Scholar 

  • McNamara JC, Torres AH (1999) Ultracytochemical location of Na+/K+-ATPase activity and effects of high salinity acclimation in gill and renal epithelia of the freshwater shrimp Macrobrachium olfersii (Crustacea, Decapoda). J Exp Zool 284:617–628

    Article  PubMed  CAS  Google Scholar 

  • McNamara JC, Moreira GS, Moreira OS (1983) The effect of salinity on respiratory metabolism, survival and moulting in the first zoea of Macrobrachium amazonicum (Heller) (Crustacea, Palaemonidae). Hydrobiologia 101:239–242

    Article  Google Scholar 

  • McNamara JC, Moreira GS, Souza SCR (1986) The effect of salinity on respiratory metabolism in selected ontogenetic stages of the freshwater shrimp Macrobrachium olfersii (Decapoda, Palaemonidae). Comp Biochem Physiol A 83:359–363

    Article  Google Scholar 

  • Mendonça NN, Masui DC, McNamara JC, Leone FA, Furriel RPM (2007) Long-term exposure of the freshwater shrimp Macrobrachium olfersi to elevated salinity: effects on gill (Na+,K+)-ATPase α-subunit expression and K+-phosphatase activity. Comp Biochem Physiol A 146:534–543

    Article  Google Scholar 

  • Moraes-Valenti P, Valenti WC (2010) Culture of the Amazon River prawn Macrobrachium amazonicum. In: New MB, Valenti WC, Tidwel JH, Abramo LR, Kutty MN (eds) Freshwater prawns: biology and farming. Wiley, Oxford, pp 485–501

    Google Scholar 

  • Moreira GS, McNamara JC, Moreira PS (1986) The effect of salinity on the upper thermal limits of survival and metamorphosis during larval development in Macrobrachium amazonicum (Heller) (Decapoda, Palaemonidae). Crustaceana 50:231–238

    Article  Google Scholar 

  • Moretti R, Martin M, Proverbio T, Proverbio F, Marin R (1991) Ouabain-insensitive Na-ATPase activity in homogenates from different animal tissues. Comp Biochem Physiol B 98:623–626

    Article  PubMed  CAS  Google Scholar 

  • Morth JP, Pedersen BP, Toustrup-Jensen MS, Sorensen TLM, Petersen J, Andersen JP, Vilsen B, Nissen P (2007) Crystal structure of the sodium–potassium pump. Nature 450:1043–1050

    Article  PubMed  CAS  Google Scholar 

  • Onken H, McNamara JC (2002) Hyperosmoregulation in the red freshwater crab Dilocarcinus pagei (Brachyura, Trichodactylidae): structural and functional asymmetries of the posterior gills. J Exp Biol 205:167–175

    PubMed  Google Scholar 

  • Onken H, Riestenpatt S (1998) NaCl absorption across split gill lamellae of hyperregulating crabs, transport mechanisms and their regulation. Comp Biochem Physiol A 119:883–893

    Article  Google Scholar 

  • Onken H, Graszynski K, Johansen A, Putzenlechner M, Riestenpatt S, Schirmer C, Siebers D, Zeiske W (1995) How to overcome osmotic stress: marine crabs conquer fresh-water. New insights from modern electrophysiology. Helgol Meeresunters 49:715–725

    Article  Google Scholar 

  • Pagliarani A, Bandiera P, Ventrella V, Trombetti F, Manuzzi MP, Pirini M, Borgatti AR (2008) Response of Na+-dependent ATPase activities to the contaminant ammonia nitrogen in Tapes philippinarum: possible ATPase involvement in ammonium transport. Arch Environ Contam Toxicol 55:49–56

    Article  PubMed  CAS  Google Scholar 

  • Péqueux A (1995) Osmotic regulation in crustaceans. J Crust Biol 15:1–60

    Article  Google Scholar 

  • Pettovello AD (1996) First record of Macrobrachium amazonicum (Decapoda, Palaemonidae) in Argentina. Crustaceana 69:113–116

    Article  Google Scholar 

  • Poulsen H, Morth PO, Egebjerg J, Nissen P (2010) Phosphorylation of the Na+,K+-ATPase and the H+,K+-ATPase. FEBS Lett 584:2589–2595

    Article  PubMed  CAS  Google Scholar 

  • Proverbio F, Marin R, Proverbio T (1991) The ouabain-insensitive sodium pump. Comp Biochem Physiol A 99:279–283

    Article  PubMed  CAS  Google Scholar 

  • Ramos-Porto M, Coelho PA (1998) Malacostraca Eucarida Caridea (Alpheoidea). In: Young PS (ed) Catalogue of Crustacea of Brazil. Museu Nacional, Rio de Janeiro, pp 325–350

    Google Scholar 

  • Read GHL (1984) Intraspecific variation in the osmoregulatory capacity of larval, post larval, juvenile and adult Macrobrachium petersi (Hilgendorf). Comp Biochem Physiol A 78:501–506

    Article  Google Scholar 

  • Read SM, Northcote DH (1981) Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem 116:53–64

    Article  PubMed  CAS  Google Scholar 

  • Rebelo MF, Santos EA, Monserrat JM (1999) Ammonia exposure of Chasmagnathus granulata (Crustacea, Decapoda) Dana, 1851: accumulation in haemolymph and effects on osmoregulation. Comp Biochem Phys A 122:429–435

    Article  Google Scholar 

  • Reyes A, Galindo MM, Garcia L, Segura-Pena D, Caruso-Neves C, Eblen-Zajjur A, Proverbio T, Marini R, Proverbio F (2009) Ouabain-insensitive, Na+-stimulated ATPase of several rat tissues: activity during a 24 h period. Physiol Res 58:693–699

    PubMed  CAS  Google Scholar 

  • Riestenpatt S, Onken H, Siebers D (1996) Active absorption of Na+ and Cl− across the gill epithelium of the shore crab Carcinus maenas: voltage-clamp and ion-flux studies. J Exp Biol 199:1545–1554

    PubMed  CAS  Google Scholar 

  • Robinson JD, Pratap PR (1991) Na+/K+-ATPase—modes of inhibition by Mg2+. Biochim Biophys Acta 1061:267–278

    Article  PubMed  CAS  Google Scholar 

  • Rudolph FB, Baugher BW, Beissner RS (1979) Techniques in coupled enzyme assays. Methods Enzymol 63:22–42

    Article  PubMed  CAS  Google Scholar 

  • Sáez AG, Lozano E, Zaldívar-Riverón A (2009) Evolutionary history of Na,K-ATPases and their osmoregulatory role. Genetica 136:479–490

    Article  PubMed  Google Scholar 

  • Sandifer PA, Hopkins JS, Smith TIJ (1975) Observations on salinity tolerance and osmoregulation in laboratory reared Macrobrachium rosenbergii post-larvae (Crustacea, Caridea). Aquaculture 6:103–114

    Article  Google Scholar 

  • Santos LCF, Belli NM, Augusto A, Masui DC, Leone FA, McNamara JC, Furriel RPM (2007) Gill (Na+,K+)-ATPase in diadromous, freshwater palaemonid shrimps: species specific kinetic characteristics and α-subunit expression. Comp Biochem Physiol A 148:178–188

    Article  CAS  Google Scholar 

  • Short JW (2004) A revision of Australian river prawns, Macrobrachium (Crustacea: Decapoda: Palaemonidae). Hydrobiologia 525:1–100

    Article  Google Scholar 

  • Stern S, Borut A, Cohen D (1984) Characterization of (Na,K)-ATPase from the gills of the freshwater prawn Macrobrachium rosenbergii (De Man). Comp Biochem Physiol B 79:47–50

    Article  Google Scholar 

  • Sun DY, Guo JZ, Hartman HA, Uno H, Hokin LE (1991) Na,K-ATPase expression in the developing brine shrimp Artemia. Immunochemical localization of the alpha- and beta-subunits. J Histochem Cytochem 39:1455–1460

    Article  PubMed  CAS  Google Scholar 

  • Susanto GN, Charmantier G (2001) Crayfish freshwater adaptation starts in eggs: ontogeny of osmoregulation in embryos of Astacus leptodactylus. J Exp Zool 289:433–440

    Article  PubMed  CAS  Google Scholar 

  • Tentes I, Stratakis E (1991) Partial purification and properties of Na,K-ATPase from Potamon potamios. Comp Biochem Physiol C 100:619–624

    Article  PubMed  CAS  Google Scholar 

  • Therien AG, Nestor NB, Ball NJ, Blostein R (1996) Tissue-specific versus isoform-specific differences in cation activation kinetics of the Na,K-ATPase. J Biol Chem 271:7104–7112

    Article  PubMed  CAS  Google Scholar 

  • Thuet P, Charmantier-Daures M, Charmantier G (1988) Relation entre osmorégulation et activité d’ATPase Na+–K+ et d’anhydrase carbonique chez les larves et post-larves de Homarus gammarus (L.) (Crustacea, Decapoda). J Exp Mar Biol Ecol 115:249–261

    Article  CAS  Google Scholar 

  • Torres AH, Onken H, McNamara JC (2003) Como as brânquias de um camarão de água doce capturam Na+, Ca2+ e Cl− do meio ambiente: uma abordagem eletrofisiológica qualitativa. In: XVIII Reunião Anual da Federação das Sociedades de Biologia Experimental, Curitiba, Brazil, 23–27 August 2003. Abstract 11.022

  • Towle DW, Holleland T (1987) Ammonium ion substitutes for K+ in ATP-dependent Na+ transport by basolateral membrane vesicles. Am J Physiol Regul Integr Comp Physiol 252:R427–R489

    Google Scholar 

  • Wall SM (1996) Ammonium transport and the role of the Na,K-ATPase. Miner Electrolyte Metab 22:311–317

    PubMed  CAS  Google Scholar 

  • Weihrauch D, Becker W, Postel U, Luck-Kopp S, Siebers D (1999) Potential of active excretion of ammonia in three different haline species of crabs. J Comp Physiol B 169:364–376

    Article  Google Scholar 

  • Weihrauch D, Ziegler A, Siebers D, Towle DW (2002) Active ammonia excretion across the gills of the green shore crab Carcinus maenas: participation of Na+/K+-ATPase, V-type H+-ATPase and functional microtubules. J Exp Biol 205:2765–2775

    PubMed  CAS  Google Scholar 

  • Weihrauch D, McNamara JC, Towle D, Onken H (2004a) Ion-motive ATPases and active transbranchial NaCl uptake in the red freshwater crab Dilocarcinus pagei (Decapoda, Trichodactylidae). J Exp Biol 207:4623–4631

    Article  PubMed  CAS  Google Scholar 

  • Weihrauch D, Morris S, Towle DW (2004b) Ammonia excretion in aquatic and terrestrial crabs. J Exp Biol 207:4491–4504

    Article  PubMed  CAS  Google Scholar 

  • Wilder MN, Huong DTT, Okuno A, Atmomarsono M, Yang WJ (2001) Ouabain-sensitive Na/K-ATPase activity increases during embryogenesis in the giant freshwater prawn Macrobrachium rosenbergii. Fish Sci 67:182–184

    Article  CAS  Google Scholar 

  • Zanders IP, Rodriguez JM (1992) Effects of temperature and salinity stress on osmoionic regulation in adults and on oxygen-consumption in larvae and adults of Macrobrachium amazonicum (Decapoda, Palaemonidae). Comp Biochem Physiol A 101:505–509

    Article  Google Scholar 

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Acknowledgements

This investigation was supported by research grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Instituto Nacional de Ciência e Tecnologia (INCT) ADAPTA/Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM, 573976/2008-2). T. M. S. B. received undergraduate scholarships from CNPq; A. S. A., D. C. M. and D. P. G. received postdoctoral scholarships from FAPESP. F. A. L., W. C. V. and J. C. M. received research scholarships from CNPq. We thank Nilton Rosa Alves and Roberto Polachini for technical assistance. This laboratory (F. A. L.) is integrated with the Amazon Shrimp Network (Rede de Camarão da Amazônia) and with ADAPTA (Centro de Estudos de Adaptações da Biota Aquática da Amazônia).

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  1. Daniela Pereira Garçon

    Present address: Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, 58059-900, Brazil

Authors and Affiliations

  1. Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes, 3900, Ribeirão Prêto, SP, 14040-901, Brazil

    Francisco Assis Leone, Douglas Chodi Masui, Thais Milena de Souza Bezerra & Daniela Pereira Garçon

  2. Campus Experimental do Litoral Paulista, Universidade Estadual Paulista Júlio de Mesquita Filho, São Vicente, SP, 11330-900, Brazil

    Wagner Cotroni Valenti & Alessandra Silva Augusto

  3. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Prêto, SP, 14040-901, Brazil

    John Campbell McNamara

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  1. Francisco Assis Leone
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  2. Douglas Chodi Masui
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  3. Thais Milena de Souza Bezerra
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  4. Daniela Pereira Garçon
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  5. Wagner Cotroni Valenti
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  6. Alessandra Silva Augusto
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  7. John Campbell McNamara
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Correspondence to Francisco Assis Leone.

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Leone, F.A., Masui, D.C., de Souza Bezerra, T.M. et al. Kinetic Analysis of Gill (Na+,K+)-ATPase Activity in Selected Ontogenetic Stages of the Amazon River Shrimp, Macrobrachium amazonicum (Decapoda, Palaemonidae): Interactions at ATP- and Cation-Binding Sites. J Membrane Biol 245, 201–215 (2012). https://doi.org/10.1007/s00232-012-9431-9

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  • DOI: https://doi.org/10.1007/s00232-012-9431-9

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