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Immunolocalization of Heat Shock Protein 27 in Developing Jaw Bones and Tooth Germs of Human Fetuses

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Abstract

27 kDa Heat shock protein (Hsp27), which is also identified as p29 estrogen-receptor associated protein, plays a crucial role in specific growth stages. It also seems to be involved in the balance between differentiation and apoptosis. To determine whether Hsp27 is involved during craniofacial development and odontogenesis, its expression was studied through immunohistochemistry of developing jaw bone as well as the odontogenesis of heads from human fetuses. Formalin-fixed paraffin-embedded specimens of 7 human fetuses (3 female, 4 male), obtained from miscarriages occurring between the 9th and 16th weeks of pregnancy, were examined by using a monoclonal antibody against Hsp27. Staining intensity (weak, +; moderate, ++; strong, +++) was evaluated semiquantitatively. The sample slice was cut through a coronal plane, which included eyes, nasal cavities, tongue, and primitive dental lamina with tooth germs. A transient and spatially restricted expression of Hsp27 in developing human jaw bones and teeth was observed. Osteoblasts around the uncalcified bone matrix showed Hsp27 immunoreaction products (+++), whereas osteocytes were not immunolabeled. In mandibular condyle, immunolabeling was restricted to hypertrophic chondrocytes (++). In developing tooth germs, Hsp27 immunostaining was detected throughout the bud (+++). At the early cap stage, a strong immunolabeling for Hsp27 was seen in the dental lamina (+++), and a moderate staining was seen in the outer dental epithelium (++). At the late cap stage, Hsp27 expression was detected in the outer dental epithelium (++) as well as in the cells of the future stellate reticulum (++). The spatiotemporal-restricted expression of Hsp27 in craniofacial bones during development suggests that this protein could be involved in the balance between differentiation and apoptosis, by modulating the viability of osteoblasts and chondrocytes. The specific regional and temporal expression patterns of Hsp27 during tooth development sustains that this small Hsp might be related to the morphogenesis and cytodifferentiation processes of tooth germs.

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Acknowledgements

The paper was supported by an intergovernmental framework for European Co-operation in the field of Scientific and Technical Research, “Cost Action B23.”

The authors wish to thank Prof. Henry Magloire for his precious scientific advice.

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Authors and Affiliations

  1. Department of Medical and Surgical Sciences, II Dental Unit, University of Catania, Catania, Italy

    R. Leonardi

  2. Institute of Dental Sciences, University of Rome, Rome, Italy

    E. Barbato

  3. Institute of Dental Sciences, University of Brescia, Brescia, Italy

    C. Paganelli

  4. Institute of Dental Sciences, Faculty of Medicine, University of Ancona, Ancona, Italy

    L. Lo Muzio

  5. Istituto di II Clinica Odontoiatrica. Policlinico. Citta’ Universitaria, Università di Catania, Via S. Sofia n. 78, Catania, Italy

    R. Leonardi

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  1. R. Leonardi
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  2. E. Barbato
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  3. C. Paganelli
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Correspondence to R. Leonardi.

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Leonardi, R., Barbato, E., Paganelli, C. et al. Immunolocalization of Heat Shock Protein 27 in Developing Jaw Bones and Tooth Germs of Human Fetuses. Calcif Tissue Int 75, 509–516 (2004). https://doi.org/10.1007/s00223-004-0077-1

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