Skip to main content
Log in

Alternative protein from Pereskia aculeata Miller leaf mucilage: technological potential as an emulsifier and fat replacement in processed mortadella meat

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

Considering that bio-based ingredients have attracted the attention of food industries and the great demand for emulsifiers, the present work studied the mucilage of Pereskia aculeata Miller (OPN) as an alternative emulsifier in meat product. Aqueous extraction by physical process was used to obtain mucilage from the leaves of the plant with high emulsifying activity. The OPN mucilage was characterized by its proximal composition, emulsifying potential and amino acid profile and was evaluated as an emulsifier and fat replacement in processed meat. Different formulations of mortadella-type meat product were developed and studied in terms of nutritional composition, color, texture profile, product-emulsion stability, firmness and sensory acceptance. OPN mucilage had a high emulsifying potential, is rich in minerals, and contains all the essential amino acids in its composition, which characterizes it as a nutritionally attractive food ingredient. The use of this mucilage allowed the replacement of chicken skin, which is an emulsifier traditionally used in mortadella-type meat product, contributing to the reduction of the overall fat content of the product. The selected formulation showed high sensory acceptance demonstrating the great potential for using OPN mucilage in meat products and as food ingredient.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Availability of data and material

Available if requested.

References

  1. Conceição MC, Junqueira LA, Guedes Silva KC et al (2014) Thermal and microstructural stability of a powdered gum derived from Pereskia aculeata Miller leaves. Food Hydrocoll 40:104–114. https://doi.org/10.1016/j.foodhyd.2014.02.015

    Article  CAS  Google Scholar 

  2. Mazon S, Menin D, Cella BM et al (2020) Exploring consumers’ knowledge and perceptions of unconventional food plants: case study of addition of Pereskia aculeata Miller to ice cream. Food Sci Technol 40:215–221. https://doi.org/10.1590/fst.39218

    Article  Google Scholar 

  3. Lima Junior FA, Conceição MC, Vilela de Resende J et al (2013) Response surface methodology for optimization of the mucilage extraction process from Pereskia aculeata Miller. Food Hydrocoll 33:38–47. https://doi.org/10.1016/j.foodhyd.2013.02.012

    Article  CAS  Google Scholar 

  4. Takeiti CY, Antonio GC, Motta EMP et al (2009) Nutritive evaluation of a non-conventional leafy vegetable (Pereskia aculeata Miller). Int J Food Sci Nutr 60:148–160. https://doi.org/10.1080/09637480802534509

    Article  CAS  PubMed  Google Scholar 

  5. Silva SH, Neves ICO, Oliveira NL et al (2019) Extraction processes and characterization of the mucilage obtained from green fruits of Pereskia aculeata Miller. Ind Crops Prod 140:111716. https://doi.org/10.1016/j.indcrop.2019.111716

    Article  CAS  Google Scholar 

  6. Martin AA, de Freitas RA, Sassaki GL et al (2017) Chemical structure and physical-chemical properties of mucilage from the leaves of Pereskia aculeata. Food Hydrocoll 70:20–28. https://doi.org/10.1016/j.foodhyd.2017.03.020

    Article  CAS  Google Scholar 

  7. WHO (2018) WHO plan to eliminate industrially-produced trans-fatty acids from global food supply. In: World Health Organ. https://www.who.int/news-room/detail/14-05-2018-who-plan-to-eliminate-industrially-produced-trans-fatty-acids-from-global-food-supply. Accessed 22 Jun 2020

  8. Ribeiro JS, Santos MJMC, Silva LKR et al (2019) Natural antioxidants used in meat products: a brief review. Meat Sci 148:181–188. https://doi.org/10.1016/j.meatsci.2018.10.016

    Article  CAS  PubMed  Google Scholar 

  9. Schilling MW (2019) Emulsifier applications in meat products. In: Hasenhuettl GL, Hartel RW (eds) Food emulsifiers and their applications. Springer International Publishing, Cham, pp 347–377

    Chapter  Google Scholar 

  10. FAO (1985) Production of emulsion-type sausages. In: FOOD Agric. Organ. U. N. http://www.fao.org/3/x6556e/X6556E00.htm#TOC. Accessed 22 Jun 2020

  11. McArdle R, Hamill R (2011) Utilisation of hydrocolloids in processed meat systems. Processed meats. Elsevier, New York, pp 243–269

    Chapter  Google Scholar 

  12. Garcia-Torchelsen L, Jacob-Lopes E, Queiroz MI (2011) Avaliação funcional de bases proteicas desidratadas de anchoita (Engraulis anchoita). Braz J Food Technol 14:283–293. https://doi.org/10.4260/BJFT2011140400034

    Article  CAS  Google Scholar 

  13. White JA, Hart RJ, Fry JC (1986) An evaluation of the Waters Pico-Tag system for the amino-acid analysis of food materials. J Autom Chem 8:170–177. https://doi.org/10.1155/S1463924686000330

    Article  CAS  Google Scholar 

  14. Terra NN, Fries LLM, Milani LIG et al (2009) Emprego de soro de leite líquido na elaboração de mortadela. Ciênc Rural 39:885–890. https://doi.org/10.1590/S0103-84782009000300038

    Article  CAS  Google Scholar 

  15. AOAC (2005) Official methods of analysis of the association of analytical chemists international, 18th edn. Official Methods, Gathersburg

    Google Scholar 

  16. Bligh GE, Dyer JW (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  Google Scholar 

  17. Merrill A, Watt B (1973) Energy value of foods - basis and derivation. USDA Agric Res Serv Hum Nutr Res Branch 74:109

    Google Scholar 

  18. Ávila MDR, Isabel Cambero M, Ordóñez JA et al (2014) Rheological behaviour of commercial cooked meat products evaluated by tensile test and texture profile analysis (TPA). Meat Sci 98:310–315. https://doi.org/10.1016/j.meatsci.2014.05.003

    Article  Google Scholar 

  19. ISO 13299 (2016) Sensory analysis—methodology—general guidance for establishing a sensory profile. In: ISO 13299:2016, 1st ed. ANSI, New York, p 41

  20. Marques C, Lise CC, de Lima VA, Mitterer-Daltoé ML (2020) Survival analysis and cut-off point to estimate the shelf life of refrigerated fish burgers. Food Sci Technol 40:171–177. https://doi.org/10.1590/fst.36918

    Article  Google Scholar 

  21. Soukoulis C, Gaiani C, Hoffmann L (2018) Plant seed mucilage as emerging biopolymer in food industry applications. Curr Opin Food Sci 22:28–42. https://doi.org/10.1016/j.cofs.2018.01.004

    Article  Google Scholar 

  22. Mitterer-Daltoé ML, Carrillo E, Queiroz MI et al (2013) Structural equation modelling and word association as tools for a better understanding of low fish consumption. Food Res Int 52:56–63. https://doi.org/10.1016/j.foodres.2013.02.048

    Article  Google Scholar 

  23. Garcia JAA, Corrêa RCG, Barros L et al (2019) Phytochemical profile and biological activities of “Ora-pro-nobis” leaves (Pereskia aculeata Miller), an underexploited superfood from the Brazilian Atlantic Forest. Food Chem 294:302–308. https://doi.org/10.1016/j.foodchem.2019.05.074

    Article  CAS  PubMed  Google Scholar 

  24. Damodaran S, Parkin KL, Fennema OR (2007) Fennema’s food chemistry, 4th edn. CRC Press, Boca Raton

    Google Scholar 

  25. Yoshimura Y, Bise T, Shimazu S et al (2019) Effects of a leucine-enriched amino acid supplement on muscle mass, muscle strength, and physical function in post-stroke patients with sarcopenia: a randomized controlled trial. Nutrition 58:1–6. https://doi.org/10.1016/j.nut.2018.05.028

    Article  CAS  PubMed  Google Scholar 

  26. Marques C, Reis A, Moura C et al (2018) Consumer insight into the monosodium glutamate. Acta Sci. https://doi.org/10.4025/actascitechnol.v40i1.30838

    Article  Google Scholar 

  27. Jideani VA, Bello BM (2009) Functional properties of okra protein products containing different levels of mucilage. J Food Agric Environ 7:5

    Google Scholar 

  28. Câmara AKFI, Okuro PK, da Cunha RL et al (2020) Chia (Salvia hispanica L.) mucilage as a new fat substitute in emulsified meat products: technological, physicochemical, and rheological characterization. LWT 125:109193. https://doi.org/10.1016/j.lwt.2020.109193

    Article  CAS  Google Scholar 

  29. BRASIL (2000) Instrução normativa n.4, 31 março de 2000. Aprova os Regulamentos Técnicos de Identidade e Qualidade de Carne Mecanicamente Separada, de Mortadela, de Linguiça e de Salsicha. In: Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Diário Oficial da República Federativa do Brasil, Brasília, Brazil, pp 6–10

  30. Mercê A (2001) Complexes of arabinogalactan of Pereskia aculeata and Co2+, Cu2+, Mn2+, and Ni2+. Bioresour Technol 76:29–37. https://doi.org/10.1016/S0960-8524(00)00078-X

    Article  PubMed  Google Scholar 

  31. Elias FO, Capitani CD, Molina S et al (2006) Emulsifying properties of whey protein-polysaccharide complexes. Braz J Food Technol IIIJIPCA

  32. Yang H-S, Choi S-G, Jeon J-T et al (2007) Textural and sensory properties of low fat pork sausages with added hydrated oatmeal and tofu as texture-modifying agents. Meat Sci 75:283–289. https://doi.org/10.1016/j.meatsci.2006.07.013

    Article  CAS  PubMed  Google Scholar 

  33. McLAREN K (2008) XIII-the development of the CIE 1976 (L*a*b*) uniform colour space and colour-difference formula. J Soc Dye Colour 92:338–341. https://doi.org/10.1111/j.1478-4408.1976.tb03301.x

    Article  Google Scholar 

  34. Amaral TN, Junqueira LA, Prado MET et al (2018) Blends of Pereskia aculeata Miller mucilage, guar gum, and gum Arabic added to fermented milk beverages. Food Hydrocoll 79:331–342. https://doi.org/10.1016/j.foodhyd.2018.01.009

    Article  CAS  Google Scholar 

  35. Oliveira NL, Rodrigues AA, Oliveira Neves IC et al (2019) Development and characterization of biodegradable films based on Pereskia aculeata Miller mucilage. Ind Crops Prod 130:499–510. https://doi.org/10.1016/j.indcrop.2019.01.014

    Article  CAS  Google Scholar 

  36. Armaforte E, Hopper L, Stevenson G (2021) Preliminary investigation on the effect of proteins of different leguminous species (Cicer arietinum, Vicia faba and Lens culinarius) on the texture and sensory properties of egg-free mayonnaise. LWT 136:110341. https://doi.org/10.1016/j.lwt.2020.110341

    Article  CAS  Google Scholar 

  37. Lam RSH, Nickerson MT (2013) Food proteins: a review on their emulsifying properties using a structure–function approach. Food Chem 141:975–984. https://doi.org/10.1016/j.foodchem.2013.04.038

    Article  CAS  PubMed  Google Scholar 

  38. Bueno AS, Pereira CM, Menegassi B et al (2009) Effect of extrusion on the emulsifying properties of soybean proteins and pectin mixtures modelled by response surface methodology. J Food Eng 7:504–510

    Article  Google Scholar 

  39. Schwenke KD (2001) Reflections about the functional potential of legume proteins: a review. Nahrung 45:377

    Article  CAS  Google Scholar 

  40. Liu J, Ru Q, Ding Y (2012) Glycation a promising method for food protein modification: physicochemical properties and structure, a review. Food Res Int 49:170–183. https://doi.org/10.1016/j.foodres.2012.07.034

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank UTFPR—Pato Branco and CAPES and acknowledge the technical support provided. Thanks also to Robert Lee for his assistance with the English in the manuscript.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Marina Leite Mitterer-Daltoé.

Ethics declarations

Conflict of interest

None.

Compliance with Ethics requirements

This article does not contain any studies with animal subjects or humans, besides the sensory evaluation.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 17 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lise, C.C., Marques, C., da Cunha, M.A.A. et al. Alternative protein from Pereskia aculeata Miller leaf mucilage: technological potential as an emulsifier and fat replacement in processed mortadella meat. Eur Food Res Technol 247, 851–863 (2021). https://doi.org/10.1007/s00217-020-03669-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-020-03669-8

Keywords

Navigation