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Quality and origin characterisation of Portuguese, Greek, Oceanian, and Asian honey, based on poly-parametric analysis hand in hand with dimension reduction and classification techniques

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Abstract

Chestnut, heather, honeydew, multifloral, orange blossom and thyme honey samples obtained either from beekeepers or supermarkets from Portugal, Greece, Oceania and Asia, were characterised by means of poly-parametric analysis: palynological, °Brix, moisture, pH, electrical conductivity, free acidity, total dissolved solids, salinity, vitamin C, specific weight, CIELAB colour parameters, Pfund and colour intensity determinations, along with bio-activity and volatile compounds analyses were carried out. Results showed that the botanical and geographical origin of honey samples had a similar impact on the determined parameters, using dimension reduction and classification techniques. Among them, moisture, °Brix, electrical conductivity, salinity, pH, colour parameters L* and b*, colour intensity, 1-hydroxy-2-propanone, benzaldehyde, 2-ethyl-1-hexanol, HMF and benzeneacetaldehyde were the potential markers related to the geographical and botanical origin of six honey varieties from different countries. Finally, the identification of methylglyoxal in Portuguese honeydew honey creates the basis for new research on the isolation and identification of this compound in European honey.

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References

  1. EU Council Council Directive 2001/110/EC of 20 December 2001 relating to honey. Official Journal of the European Communities 2002, L10, 47–52

  2. Von der Ohe W, Oddo PL, Piana ML, Morlot M, Martin P (2004) Harmonized methods of melissopalynology. Apidologie 35:S18–S25

    Article  Google Scholar 

  3. Pita-Calvo C, Vázquez M (2018) Honeydew honeys: a review on the characterization and authentication of botanical and geographical origins. J Agric Food Chem 66(11):2523–2537

    Article  CAS  PubMed  Google Scholar 

  4. Piljac-Žegarac J, Stipčević T, Belšča A (2009) Antioxidant properties and phenolic content of different floral origin honeys. J ApiProduct ApiMed Sci 1(2):43–50

    Article  Google Scholar 

  5. Alves A, Ramos A, Gonçalves MM, Bernardo M, Mendes B (2013) Antioxidant activity, quality parameters and mineral content of Portuguese monofloral honeys. J Food Compos Anal 30:130–138

    Article  CAS  Google Scholar 

  6. Israili ZH (2014) Antimicrobial properties of honey. Am J Ther 21(4):304–323

    Article  PubMed  Google Scholar 

  7. Commission Implementing Regulation (EU) No 872/2012 of 1 October 2012 adopting the list of flavouring substances provided for by Regulation (EC) No 2232/96 of the European Parliament and of the Council, introducing it in Annex I to Regulation (EC) No 1334/2008 of the European Parliament and of the Council and repealing Commission Regulation (EC) No 1565/2000 and Commission Decision 1999/217/EC

  8. Jánosková N, Vyviurska O, Spánik I (2014) Identification of volatile organic compounds in honeydew honeys using comprehensive gas chromatography. J Food Nutr Res 53(4):353–362

    Google Scholar 

  9. Ciappini M, Vitelleschi M, Calviño A (2016) Chemometrics classification of Argentine clover and eucalyptus honeys according to palynological, physicochemical, and sensory properties. Int J Food Proper 19(1):111–123

    Article  CAS  Google Scholar 

  10. Karabagias IK, Nikolaou C, Karabagias VK (2019) Volatile fingerprints of common and rare honeys produced in Greece: in search of PHVMs with implementation of the honey code. Eur Food Res Technol 245(1):23–39

    Article  CAS  Google Scholar 

  11. Karabagias IK, Maia M, Karabagias VK, Gatzias I, Badeka A (2018) Characterization of eucalyptus, chestnut and heather honeys from Portugal using multi-parameter analysis and chemo-calculus. Foods 7(12):194

    Article  CAS  PubMed Central  Google Scholar 

  12. Harmonised methods of the International Honey Commission (IHC, 2009) Responsible for the methods: Stefan Bogdanov, Bee Product Science. www.bee-hexagon.net

  13. Karabagias IK, Dimitriou E, Kontakos S, Kontominas MG (2016) Phenolic profile, colour intensity, and radical scavenging activity of Greek unifloral honeys. Eur Food Res Technol 242(8):1201–1210

    Article  CAS  Google Scholar 

  14. Ferreira ICFR, Aires E, Barreira JCM, Estevinho LM (2009) Antioxidant activity of Portuguese honey samples: different contributions of the entire honey and phenolic extract. Food Chem 114:1438–1443

    Article  CAS  Google Scholar 

  15. Karabagias IKK (2019) Seeking of reliable markers related to Greek nectar honey geographical and botanical origin identification based on sugar profile by HPLC-RI and electro-chemical parameters using multivariate statistics. Eur Food Res Technol 245(4):805–816

    Article  CAS  Google Scholar 

  16. Fawcett T (2006) An introduction to ROC analysis. Pattern Recognit Lett 27:861–874

    Article  Google Scholar 

  17. Louveaux J, Maurizio A, Vorwohl G (1978) Methods of melissopalynology. Bee World 59:139–157

    Article  Google Scholar 

  18. Yang Y, Battesti M-J, Djabou N, Muselli A, Paolini J, Tomi P, Costa J (2012) Melissopalynological origin determination and volatile composition analysis of Corsican “chestnut grove” honeys. Food Chem 132(4):2144–2154

    Article  CAS  Google Scholar 

  19. Estevinho L, Pereira AP, Moreira L, Dias LG, Pereira E (2008) Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food Chem Toxicol 46:3774–3779

    Article  CAS  PubMed  Google Scholar 

  20. Silva LR, Videira R, Monteiro AP, Valentão P, Andrade PB (2009) Honey from Luso region (Portugal): physicochemical characteristics and mineral contents. Microchem J 93(1):73–77

    Article  CAS  Google Scholar 

  21. Feás X, Pires J, Estevinho ML, Iglesias A, De Araujo JPP (2010) Palynological and physicochemical data characterisation of honeys produced in the Entre-Douro e Minho region of Portugal. Int J Food Sci Technol 45:1255–1262

    Article  CAS  Google Scholar 

  22. Estevinho LM, Feás X, Seijas JA, Vázquez-Tato MP (2012) Physicochemical and pollen analysis of local honeys from the Lima Valley (Portugal). Electron J Environ Agric Food Chem 11:214–221

    Google Scholar 

  23. Estevinho LM, Feás X, Seijas JA, Pilar Vázquez-Tato MP (2012) Organic honey from Trás-os-montes region (Portugal): chemical, palynological, microbiological and bioactive compounds characterization. Food Chem Toxicol 50:258–264

    Article  CAS  PubMed  Google Scholar 

  24. Pires J, Estevinho ML, Feás X, Cantalapiedra J, Iglesias A (2009) Pollen spectrum and physico-chemical attributes of heather (Erica sp.) honeys of North Portugal. J Sci Food Agric 89:1862–1870

    Article  CAS  Google Scholar 

  25. Gomes S, Dias LG, Moreira LL, Rodrigues P, Estevinho L (2010) Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food Chem Toxicol 48:544–545

    Article  CAS  PubMed  Google Scholar 

  26. Karabagias IK, Louppis A, Kontakos S, Drouza C, Papastephanou C (2018) Characterization and botanical differentiation of monofloral and multifloral honeys produced in Cyprus, Greece and Egypt using physicochemical parameter analysis and mineral content, in conjunction with supervised statistical techniques. J Anal Methods Chem 2018:10

    Article  CAS  Google Scholar 

  27. Soares S, Pinto D, Rodrigues F, Alves RC, Oliveira MBP (2017) Portuguese honeys from different geographical and botanical origins: a 4-year stability study regarding quality parameters and antioxidant activity. Molecules 22:1338

    Article  CAS  PubMed Central  Google Scholar 

  28. Mădas NM, Mărghitas LA, Dezmirean DS, Bonta V, Bobis O, Fauconnier M-L, Francis F, Haubruge E, Nguyen KB (2019) Volatile profile and physico-chemical analysis of acacia honey for geographical origin and nutritional value determination. Foods 8:445

    Article  CAS  PubMed Central  Google Scholar 

  29. White JW (1984) Instrumental color classification of honey: collaborative study. J AOAC 67:1129–1131

    Article  Google Scholar 

  30. USDA Agricultural Marketing Service (1985) United States Standards for Grades of Extracted Honey. USDA, Washington DC

  31. Aazza S, Lyoussi B, Antunes D, Miguel MG (2013) Physicochemical characterization and antioxidant activity of commercial Portuguese honeys. J Food Sci 78:C1159–C1165

    Article  CAS  PubMed  Google Scholar 

  32. Pontes M, Marques JC, Câmara JS (2007) Screening of volatile composition from Portuguese multifloral honeys using headspace solid-phase microextraction-gas chromatography-quadrupole mass spectrometry. Talanta 74:91–103

    Article  CAS  PubMed  Google Scholar 

  33. Miguel MG, Aazza S, Antunes MD, Faleiro ML, Barroso JG, Pedro LG, Figueiredo AC (2016) Mineral and volatile composition of água-mel from Portugal. Eur Food Res Technol 242:171–178

    Article  CAS  Google Scholar 

  34. Tamura H, Appel M, Richling E, Schreier P (2005) Authenticity assessment of γ- and δ-decalactone from Prunus fruits by gas chromatography combustion/pyrolysis isotope ratio mass spectrometry (GC-C/P-IRMS). J Agric Food Chem 53(13):5397–5401

    Article  CAS  PubMed  Google Scholar 

  35. Castro-Vázquez L, Díaz-Maroto MC, Guchu E, Pérez-Coello MS (2006) Analysis of volatile compounds of eucalypt honey by solid phase extraction followed by gas chromatography coupled to mass spectrometry. Eur Food Res Technol 224(1):27–31

    Article  CAS  Google Scholar 

  36. Surburg H, Panten J (2016) Common fragrance and flavor materials: preparation, properties and uses, 6th edn. Wiley-VCH Verlag GmbH & Co KGaA, Weinheim

    Book  Google Scholar 

  37. Mavric E, Wittmann S, Barth G, Henle T (2008) Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka (Leptospermum scoparium) honeys from New Zealand. Mol Nutr Food Res 52(4):483–489

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Ioannis K. Karabagias.

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Karabagias, I.K., Maia, M., Karabagias, V.K. et al. Quality and origin characterisation of Portuguese, Greek, Oceanian, and Asian honey, based on poly-parametric analysis hand in hand with dimension reduction and classification techniques. Eur Food Res Technol 246, 987–1006 (2020). https://doi.org/10.1007/s00217-020-03461-8

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