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Comparison of OXITEST and RANCIMAT methods to evaluate the oxidative stability in frying oils

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Abstract

The oxidation of fatty acids in the presence of atmospheric oxygen is recognized as one of the main factors affecting the shelf life of oils and fats causing rancidity by the formation of off-flavors due to aliphatic aldehydes or other volatile compounds. Therefore, the oxidative stability is one of the most important parameters to be evaluated in the formulation of commercial frying oils. In this regard, the induction period of 15 frying oils has been measured using two different methods of accelerated oxidation, OXITEST and RANCIMAT, and correlated to their fatty acid content previously detected by gas chromatographic technique. Frying oils containing high contents of saturated fatty acids mainly from the palm oil achieved an improved oxidative stability by increasing their induction period. Moreover, the linear regression analysis showed a good correlation between the induction period values of the two instruments. Hence, the innovative OXITEST method may be an easy, fast, and eco-friendly alternative to the official RANCIMAT method for evaluating the oxidative stability in oil- and fat-containing products.

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References

  1. Stier RF (2004) Frying as a science—an introduction. Eur J Lipid Sci Technol 106:715–721

    Article  CAS  Google Scholar 

  2. Oreopoulou V, Krokida M, Marinos-Kouris D (2006) In: Mujumdar AS (ed) Handbook of industrial drying, 3rd edn. CRC Press, Boca Raton

    Google Scholar 

  3. Choe E, Min DB (2007) Chemistry of deep-fat frying oils. J Food Sci 72:77–86

    Article  Google Scholar 

  4. Marmesat S, Morales A, Velasco J, Dobarganes MC (2012) Influence of fatty acid composition on chemical changes in blends of sunflower oils during thermoxidation and frying. Food Chem 135:2333–2339

    Article  CAS  Google Scholar 

  5. Aladedunye F, Przybylski R (2013) Frying stability of high oleic sunflower oils as affected by composition of tocopherol isomers and linoleic acid content. Food Chem 141:2373–2378

    Article  CAS  Google Scholar 

  6. Gliszczyńska-Swigło A, Sikorska E, Khmelinskii I, Sikorski M (2007) Tocopherol content in edible plant oils. Pol J Food Nutr Sci 57:157–161

    Google Scholar 

  7. De Marco E, Savarese M, Parisini C, Battimo I, Falco S, Sacchi R (2007) Frying performance of a sunflower/palm oil blend in comparison with pure palm oil. Eur J Lipid Sci Technol 109:237–246

    Article  Google Scholar 

  8. De Leonardis A, Macciola V (2012) Heat-oxidation stability of palm oil blended with extra virgin olive oil. Food Chem 135:1769–1776

    Article  Google Scholar 

  9. Melton SL, Jafar S, Sykes D, Trigiano MK (1994) Review of stability measurements for frying oils and fried food flavor. J Am Oil Chem Soc 71:1301–1308

    Article  CAS  Google Scholar 

  10. AOCS (1989) Official methods and recommended practices of the AOCS, 4th edn. AOCS, Champaign, Ill

    Google Scholar 

  11. Anwar F, Bhanger MI, Kazi TG (2003) Relationship between rancimat and active oxygen method values at varying temperatures. J Am Oil Chem Soc 80:151–155

    Article  CAS  Google Scholar 

  12. AOCS (2012) Official methods and recommended practices of the AOCS, 6th edn. AOCS, Champaign, Ill

    Google Scholar 

  13. Mateos R, Uceda M, Aguilera MP, Escuderos ME, Maza GB (2006) Relationship of Rancimat method values at varying temperatures for virgin olive oils. Eur J Lipid Sci Technol 223:246–252

    CAS  Google Scholar 

  14. Peer MS, Kasimani R, Rajamohan S, Ramakrishnan P (2017) Experimental evaluation on oxidation stability of biodiesel/diesel blends with alcohol addition by rancimat instrument and FTIR spectroscopy. J Mech Sci Technol 31:455–463

    Article  Google Scholar 

  15. Lante A, Friso D (2013) Oxidative stability and rheological properties of nanoemulsions with ultrasonic extracted green tea infusion. Food Res Int 54:269–276

    Article  CAS  Google Scholar 

  16. Mohammadi A, Jafari SM, Esfanjani AF, Akhavan S (2016) Application of nano-encapsulated olive leaf extract in controlling the oxidative stability of soybean oil. Food Chem 190:513–519

    Article  CAS  Google Scholar 

  17. Lante A, Nardi T, Zocca F, Giacomini A, Corich V (2011) Evaluation of red chicory extract as a natural antioxidant by pure lipid oxidation and yeast oxidative stress response as model systems. J Agric Food Chem 59:5318–5324

    Article  CAS  Google Scholar 

  18. Mihaylova DS, Lante A, Tinello F, Albert IK (2014) Study on the antioxidant and antimicrobial activities of Allium ursinum L. pressurised-liquid extract. Nat Prod Res 28:2000–2005

    Article  CAS  Google Scholar 

  19. Riciputi Y, Caboni MF (2017) Assessing oil oxidative stability in Tarallini by OXITEST®. Ital J Food Sci 29:63–73

    Google Scholar 

  20. Caruso MC, Galgano F, Colangelo MA, Condelli N, Scarpa T, Tolve R, Favati F (2017) Evaluation of the oxidative stability of bakery products by OXITEST method and sensory analysis. Eur Food Res Technol 243:1183–1191

    Article  CAS  Google Scholar 

  21. Verardo V, Riciputi Y, Sorrenti G, Ornaghi P, Marangoni B, Caboni MF (2013) Effect of nitrogen fertilisation rates on the content of fatty acids, sterols, tocopherols and phenolic compounds, and on the oxidative stability of walnuts. Food Sci Technol 50:732–738

    CAS  Google Scholar 

  22. Comandini P, Verardo V, Maiocchi P, Caboni MF (2009) Accelerated oxidation: comparative study of a new reactor with oxidation stability instrument. Eur J Lipid Sci Technol 111:933–940

    Article  CAS  Google Scholar 

  23. Caruso MC, Galgano F, Scarpa T, Ornaghi P, Favati F (2017) Accelerated shelf life studies of extra virgin olive oils using the Oxitest method. Inform 28:26–29

    Article  Google Scholar 

  24. Verardo V, Riciputi Y, Trivisonno MC, Marconi E, Caboni MF (2010) Effect of the addition of air-classified barley flours on the lipid stability of bakery products. Eur Food Res Technol 231:309–319

    Article  CAS  Google Scholar 

  25. Amato M, Caruso MC, Guzzo F, Galgano F, Commisso M, Bochicchio R, Labella R, Favati F (2015) Nutritional quality of seeds and leaf metabolites of Chia (Salvia hispanica L.) from Southern Italy. Eur Food Res Technol 241:615–625

    Article  CAS  Google Scholar 

  26. Ichihara K, Shibahara A, Yamamoto K, Nakayama T (1996) An improved method for rapid analysis of the fatty acids of glycerolipids. Lipids 31:535–539

    Article  CAS  Google Scholar 

  27. SAS (2013). SAS/STAT(R) 9.2 User’s guide, 2nd edn. SAS Institute Inc., Cary. http://support.sas.com/documentation/cdl/en/statug/63033/HTML/default/viewer.htm#glm_toc.htm. Accessed 29 June 2017

  28. Gerde JA, Hammond EG, White PJ (2011) Influence of polydimethylsiloxane on the oxygen concentration of oils at various temperatures. J Am Oil Chem Soc 88:925–929

    Article  CAS  Google Scholar 

  29. Mba OI, Dumont MJ, Ngadi M (2015) Palm oil: processing, characterization and utilization in the food industry—a review. Food Biosci 10:26–41

    Article  CAS  Google Scholar 

  30. Mancebo-Campos V, Salvador MD, Fregapane G (2014) Antioxidant capacity of individual and combined virgin olive oil minor compounds evaluated at mild temperature (25 and 40 °C) as compared to accelerated and antiradical assays. Food Chem 150:374–381

    Article  CAS  Google Scholar 

  31. Romano R, Giordano A, Vitiello S, Le Grottaglie L, Musso SS (2012) Comparison of the frying performance of olive oil and palm superolein. J Food Sci 77:519–531

    Article  Google Scholar 

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Acknowledgements

The authors would like to thank VELP Scientifica (Usmate, MB, Italy) for lending the OXITEST instrument and Dr. Paola Ornaghi for technical assistance.

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Correspondence to Fabio Favati.

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Tinello, F., Lante, A., Bernardi, M. et al. Comparison of OXITEST and RANCIMAT methods to evaluate the oxidative stability in frying oils. Eur Food Res Technol 244, 747–755 (2018). https://doi.org/10.1007/s00217-017-2995-y

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  • DOI: https://doi.org/10.1007/s00217-017-2995-y

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