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A novel α-l-rhamnosidase with potential applications in citrus juice industry and in winemaking

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Abstract

The production of monoglycosylated flavonoids by α-l-rhamnosidases (EC 3.2.1.40) is an interesting development in biocatalysis. Applications of rhamnosidases in industry include removal of bitterness caused by naringin from citrus juices. In the present work, a psychrotolerant bacterial strain with α-l-rhamnosidase activity was isolated. The α-l-rhamnosidase was found to be able to degrade naringin and was purified and characterized. The α-l-rhamnosidase from Brevundimonas sp. Ci19 was able to release both rhamnose and prunin from naringin. The enzyme was partially purified with a performance of 2.7-fold purification. The α-l-rhamnosidase showed an optimum pH between 6.00 and 7.00 with substantial residual activity at pH 5.00 (85.3 %). The optimum temperature was between 20 and 37 °C. The enzyme showed activation in the presence of Ca2+ and Cd2+ ions and at a high ethanol concentration level (10 % v/v). Activity was found for β-d-glucosidase (EC 3.2.1.21) in the partially purified extract, but it was inactive in the acid pH region. This result indicates the potential for inactivation of β-d-glucosidase along with the high level of α-l-rhamnosidase activity necessary for the production of flavonoid glycosides. The α-l-rhamnosidase from Brevundimonas sp. Ci19 showed interesting properties for potential use not only in the citrus juice industry but also in winemaking.

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Acknowledgments

The authors gratefully acknowledge financial support of CIUNT, ANPCYT, and CONICET. They are also grateful for the technical assistance provided by Dr. Ricardo Fitzsimons and Dr. Emilio Rodriguez. We would also like to thank Prof. Mirta Daz at the Universidad Nacional de Salta for donating the naringin, prunin, and naringenin used in the present work.

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), Av. Belgrano y Pje. Caseros, T4001 MVB, Tucumán, Argentina

    Adriana E. Alvarenga & Cintia M. Romero

  2. Nanobiomaterials Laboratory, Applied Biotechnology Institute (CINDEFI, UNLP-CONICET CCT La Plata), Universidad Nacional de La Plata, La Plata, Argentina

    Guillermo R. Castro

  3. Facultad de Bioquímica, Química, Farmacia y Biotecnología, Universidad Nacional de Tucumán, Ayacucho 471, T4000 INI, Tucumán, Argentina

    Cintia M. Romero

Authors
  1. Adriana E. Alvarenga
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  2. Cintia M. Romero
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  3. Guillermo R. Castro
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Corresponding author

Correspondence to Guillermo R. Castro.

Additional information

This work is dedicated in memoriam to Prof. Carlos M. Abate, colleague and friend.

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Alvarenga, A.E., Romero, C.M. & Castro, G.R. A novel α-l-rhamnosidase with potential applications in citrus juice industry and in winemaking. Eur Food Res Technol 237, 977–985 (2013). https://doi.org/10.1007/s00217-013-2074-y

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