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An immunoassay based on bio-barcode method for quantitative detection of semicarbazide

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Abstract

A functionalized gold nanoparticles bio-barcode technology was developed to detect semicarbazide. In the assay, the hapten CPSEM (4-[[2-(aminocarbonyl) hydrazinylidene] methyl]-benzoic acid) was first prepared and conjugated to bovine serum albumin as the coating antigen. Subsequently, gold nanoparticles dual-labeled with anti-CPSEM monoclonal antibodies and DNA oligonucleotide were synthesized via a one-step preparation method. Enzymatic signal was converted to DNA signal by combining polymerase chain reaction with indirect competitive ELISA (icELISA). The limit of detection of this method could reach 8 pg mL−1 for detecting SEM, about 25 times more sensitive than conventional ELISA. The immunosorbent bio-barcode assay is a rapid high-throughput screening tool adapted for ultra-high sensitive detection.

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Acknowledgments

This work was supported by the Science and Technology Plan Foundation of Guangdong Province (2005B20401008).

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Authors and Affiliations

  1. Molecular Immunology and Antibody Engineering Center, Jinan University, 510632, Guangzhou, People’s Republic of China

    Yong Tang, Lu Yan, Jun-jian Xiang, Wu-zhou Wang & Hong-yu Yang

Authors
  1. Yong Tang
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  2. Lu Yan
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  3. Jun-jian Xiang
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  4. Wu-zhou Wang
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  5. Hong-yu Yang
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Corresponding author

Correspondence to Jun-jian Xiang.

Additional information

Yong Tang and Lu Yan—Co-first author.

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Tang, Y., Yan, L., Xiang, Jj. et al. An immunoassay based on bio-barcode method for quantitative detection of semicarbazide. Eur Food Res Technol 232, 963–969 (2011). https://doi.org/10.1007/s00217-011-1462-4

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  • DOI: https://doi.org/10.1007/s00217-011-1462-4

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