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Development of a salmon protein hydrolysate that lowers blood pressure

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Abstract

A salmon protein hydrolysate (SPH) was developed containing several angiotensin I-converting enzyme (ACE) inhibitory tripeptides the most abundant of which were Val-Leu-Trp, Val-Phe-Tyr, and Leu-Ala-Phe. Simulated digestion experiments showed that active constituents of SPH would survive in the digestive tract and be available for absorption into the bloodstream. In fact, ACE inhibitory activity was improved following simulated digestion suggesting that there were larger peptides in SPH that might contribute to bioactivity in vivo. A single oral dose (1,500 mg/kg body mass) of SPH significantly lowered blood pressure in spontaneously hypertensive rats (SHR). The treatment of SHR with either SPH fraction (<3,000 Da) or SPH fraction (>3,000 Da) reduced blood pressure. We conclude that the ability of SPH to lower blood pressure is due to a combination of ACE inhibitory tripeptides as identified, as well as additional unknown, peptide species that are generated during digestion of SPH in the gastrointestinal tract.

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Acknowledgments

Ocean Nutrition Canada (Private Company) and National Research Council of Canada-Industrial Research Assistance Program (NRC-IRAP).

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Authors and Affiliations

  1. Ocean Nutrition Canada Ltd, 101 Research Dr, Dartmouth, NS, B2Y 4T6, Canada

    H. Stephen Ewart, Dorothy Dennis, Michael Potvin, Christa Tiller, Jonathan M. Curtis, Sylvie Cloutier & Colin J. Barrow

  2. National Center for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 1 Xian Nong Tan St., 100050, Beijing, China

    Lian-hua Fang, Ran Zhang, Xiao-ming Zhu & Guanhua Du

  3. National Research Council of Canada-Institute for Marine Biosciences, 1411 Oxford St., Halifax, NS, B3H 3Z1, Canada

    H. Stephen Ewart

  4. Department of Agricultural, Food and Nutritional Science, University of Alberta, 4-10 Agriculture/Forestry Centre, Edmonton, AB, T6G 2P5, Canada

    Jonathan M. Curtis

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  1. H. Stephen Ewart
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  2. Dorothy Dennis
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  3. Michael Potvin
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  4. Christa Tiller
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  5. Lian-hua Fang
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  6. Ran Zhang
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  7. Xiao-ming Zhu
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  8. Jonathan M. Curtis
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  9. Sylvie Cloutier
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  10. Guanhua Du
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  11. Colin J. Barrow
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Correspondence to H. Stephen Ewart.

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Ewart, H.S., Dennis, D., Potvin, M. et al. Development of a salmon protein hydrolysate that lowers blood pressure. Eur Food Res Technol 229, 561–569 (2009). https://doi.org/10.1007/s00217-009-1083-3

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  • DOI: https://doi.org/10.1007/s00217-009-1083-3

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