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A procedure for olive oil traceability and authenticity: DNA extraction, multiplex PCR and LDR–universal array analysis

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Abstract

Traceability of olive oils is relevant not only in assessing their origin, but also in protecting against frauds. Here, we present an improvement of the assay previously developed for the genotyping of forty-nine frequently grown Mediterranean olive cultivars by ligation detection reaction (LDR)/universal array (UA), refining the entire procedure in order to address DNA extracted from monovarietal olive oils. Firstly, a simple and robust protocol to extract amplifiable DNA from olive oil was developed. Then, the SNP-containing DNA sequences were simultaneous amplified by multiplex PCR and used on a LDR-UA platform, which gave precise and accurate genotype results. Thirteen out of the seventeen investigated SNPs were amplifiable in multiplex PCR, and were sufficient to unequivocally discriminate the forty-nine cultivars. The availability of this semi-automated SNP genotyping assay should help food testing laboratories to verify the origin and authenticity of monovarietal extra-virgin olive oils.

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Acknowledgments

This study has been carried out as part of the “Traceability of origin and authenticity of olive oil by combined genomic and metabolomic approaches” project (OLIV-TRACK, www.dsa.unipr.it/foodhealth/oliv-track/index.html) funded by the European Union (contract QLK1-CT-2002-02386, Quality of Life and Management of Living Resources). The partners of the OLIV-TRACK project are acknowledged for sharing their samples and expertise. We thank MIUR for partial financial support (FIRB “MICRAM”, RBNE01ZB7A and FIRB2003, RBLA03ER38_004). Part of this work was supported by the EU-FPV Marie Curie Training Site scheme. Our thanks to NIAB (Cambridge, UK) for their hospitality and scientific support, to Maria Vurchio for help with administrative issues and to Giada Caredda for the support in the experimental phase.

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Author notes

  1. Luisa Palmieri

    Present address: IASMA-Research Centre Agrifood Qualità Department, Via E. Mach, 38010, San Michele all’Adige (TN), Italy

Authors and Affiliations

  1. Institute of Biomedical Technologies, National Research Council, LITA, Via Fratelli Cervi 93, 20090, Segrate, MI, Italy

    Clarissa Consolandi, Marco Severgnini & Gianluca De Bellis

  2. Department of Environmental Sciences, University of Parma, Parco Area delle Scienze 11/A, 43100, Parma, Italy

    Luisa Palmieri, Elena Maestri, Nelson Marmiroli & Caterina Agrimonti

  3. Institute of Plant Genetics, National Research Council, Via Madonna Alta 130, 06128, Perugia, Italy

    Luciana Baldoni

  4. NIAB, Huntingdon Road, Cambridge, CB3 0LE, UK

    Paolo Donini

  5. Institute of Biology and Agricultural Biotechnology, National Research Council, Via Bassini 15, 20133, Milano, Italy

    Bianca Castiglioni

Authors
  1. Clarissa Consolandi
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  2. Luisa Palmieri
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  3. Marco Severgnini
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  4. Elena Maestri
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  5. Nelson Marmiroli
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  6. Caterina Agrimonti
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  7. Luciana Baldoni
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  8. Paolo Donini
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  9. Gianluca De Bellis
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  10. Bianca Castiglioni
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Corresponding author

Correspondence to Clarissa Consolandi.

Additional information

C. Consolandi and L. Palmieri equally contributed to this research paper.

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Consolandi, C., Palmieri, L., Severgnini, M. et al. A procedure for olive oil traceability and authenticity: DNA extraction, multiplex PCR and LDR–universal array analysis. Eur Food Res Technol 227, 1429–1438 (2008). https://doi.org/10.1007/s00217-008-0863-5

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  • DOI: https://doi.org/10.1007/s00217-008-0863-5

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