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Microbial and chemical analysis of a kvass fermentation

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Abstract

Kvass is a fermented cereal beverage which produced from malt, rye flour, stale rye bread, and sucrose and is consumed mainly in Eastern Europe. Moreover, it is of interest as one of the few examples of traditional, non-alcoholic cereal-based beverages. In this study, a commercial kvass sample was characterized with respect to the fermentation microflora and the concentration of microbial metabolites. Lactobacillus casei and Leuconostoc mesenteroides were present in cell counts of 7.3 × 107 and 6.0 × 10cfu mL−1, respectively. Saccharomyces cerevisiae was present in cell counts of 3.0 × 10cfu mL−1. PCR-DGGE analysis verified that all dominant fermentation organisms were cultivated. Microbial metabolites in kvass were ethanol, lactate, and acetate. One of the kvass isolates, Ln. mesenteroides FUA 3086 harboured a putative dextransucrase gene and formed dextran and isomalto-oligosaccharides from sucrose and maltose. Fermentation of model kvass wort confirmed that all kvass isolates grew in the fermentation substrate, moreover, formation of isomaltotriose by Ln. mesenteroides FUA 3086 was observed in model kvass fermentation.

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References

  1. Korolev DA (1963) Russkii kvas (Russian kvass) Moscow, Pishchepromizdat

  2. Ivanova EG, Kiseleva LV, Lenets NG (2006) Pivo I Napitki 2:50–51

    Google Scholar 

  3. Ermakov AA, Sitalo NA, Ivanov VS, Yakubovich FF, Emel’yanova NA (1982) Pishevaya Promyshlennost 4:30–31

    Google Scholar 

  4. Kosminskii GI, Tsed EA, Yakirevich LM (1996) Izvestiya Vysshikh Uchebnykh Zavedenii Pischevaya Tekhnologiya 5/6:47–48

    Google Scholar 

  5. Stolz P, Böcker G, Hammes WP, Vogel RF (1995) Z Lebensm Unters Forsch 201:91–96

    Article  CAS  Google Scholar 

  6. Müller MRA, Wolfrum G, Stolz P, Ehrmann MA, Vogel RF (2001) Food Microbiol 18:217–227

    Article  CAS  Google Scholar 

  7. Ehrmann MA, Müller MRA, Vogel RF (2003) Int J Syst Evol Microbiol 53:7–13

    Article  CAS  Google Scholar 

  8. Sandhu GS, Kline BC, Stockman L, Roberts GD (1995) J Clin Microbiol 33:2913–2919

    CAS  Google Scholar 

  9. Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K, Alatossava T (2000) Appl Environ Microbiol 66:297–303

    Article  CAS  Google Scholar 

  10. Chung CH, Day DF (2002) J Ind Microbiol Biotechnol 29:196–199

    Article  CAS  Google Scholar 

  11. Chelo IM, Zé-Zé L, Tenreiro R (2007) Int J Syst Evol Microbiol 57:276–286

    Article  CAS  Google Scholar 

  12. Van Beek S, Priest FG (2002) Appl Environ Microbiol 68:297–305

    Article  CAS  Google Scholar 

  13. Dobson CM, Chaban B, Deneer H, Ziola B (2004) Can J Microbiol 50:482–488

    Article  CAS  Google Scholar 

  14. Felis GE, Dellaglio F, Mizzi L, Torriani S (2001) Int J Syst Evol Microbiol 51:2113–2117

    CAS  Google Scholar 

  15. Vásquey A, Molin G, Petterrsson B, Antonsson M, Ahrné S (2005) Syst Appl Microbiol 25:430–441

    Article  CAS  Google Scholar 

  16. Dellaglio F, Felis GE, Torriani S (2002) Int J Syst Evol Microbiol 52:285–287

    Google Scholar 

  17. Hancioglu Ö, Karapinar M (1997) Int J Food Microbiol 35:271–274

    Article  CAS  Google Scholar 

  18. Gänzle MG (2005) In: Brandt MJ, Gänzle MG (eds) Mikrobiologie des Sauerteiges. Behr’s Verlag, Hamburg, pp 77–107

  19. Hammes WP, Brandt MJ, Francis KL, Rosenheim J, Seitter MFH, Vogelmann SA (2005) Trends Food Sci Technol 16:4–11

    Article  CAS  Google Scholar 

  20. Vogel RF, Knorr R, Müller MRA, Steudel U, Gänzle MG, Ehrmann MA (1999) Antonie Van Leeuwenhoek 76:403–411

    Article  CAS  Google Scholar 

  21. Ehrmann MA, Vogel RF (2005) Trends Food Sci Technol 16:31–42

    Article  CAS  Google Scholar 

  22. Stiles ME, Holzapfel WH (1997) Int J Food Microbiol 36:1–29

    Article  CAS  Google Scholar 

  23. Fitzsimons NA, Cogan TM, Condon S, Beresford T (1999) Appl Environ Microbiol 65:3418–3426

    CAS  Google Scholar 

  24. Walter J (2005) In: Tannock WG (ed) Probiotics, Prebiotics Caister. Academic Press, Norfolk, UK, pp 51–82

  25. Ouwehand AC, Salminen S, Isolauri E (2002) Antonie Van Leeuwenhoek 82:279–289

    Article  CAS  Google Scholar 

  26. Johansson M-L, Molin G, Jeppsson B, Nobaek S, Ahrné S, Bengmark S (1993) Appl Environ Microbiol 59:15–20

    CAS  Google Scholar 

  27. Tieking M, Korakli M, Ehrmann MA, Gänzle MG, Vogel RF (2003) Appl Environ Microbiol 69:945–952

    Article  CAS  Google Scholar 

  28. Orla-Jensen S (1943) The lactic acid bacteria (1943) Det Kongelige Danske Videnskabernes Selskab Biologiske Skrifter Bind II Nr 3 Kopenhagen

  29. van Hijum SAFT, Kralj S, Ozimek LK, Dijkhuizen L, van Geel-Schutten IGH (2006) Microbiol Mol Biol Rev 70:157–176

    Article  CAS  Google Scholar 

  30. Dols M, Simeon MR, Willemot R-M, Vignon MR, Monsan PF (1998) Carbohydr Res 305:549–559

    Article  Google Scholar 

  31. Rodrigues S ,Lona LMF, Franco TT (2005) Bioprocess Biolsyst Eng 28:9–14

    Article  CAS  Google Scholar 

  32. Seo E-S, Nam S-H, Kang H-K, Cho J-Y, Lee H-S, Ryu H-W, Kim D (2006) Enzyme Microb Technol e-pub ahead of print

  33. Van Loo J, Cummings J, Delzenne N, Englyst H, Franck A, Hopkins M, Kok N, Macfarlane G, Newton D, Quigley M, Roberfroid M, van Vliet T, van der Heuvel E (2000) Br J Nutr 81:121–132

    Google Scholar 

  34. Böhm G, Jelinek J, Stahl B, van Laere K, Knol J, Fanaro S, Moro G, Vigi V (2004) J Clin Gastroenterol 38(6S):S76–S79

    Article  Google Scholar 

  35. Rycroft CE, Jones MR, Gibson GR, Rastall RA (2001) J Appl Microbiol 91:878–887

    Article  CAS  Google Scholar 

  36. Vernazza CL, Gibson GR, Rastall RA (2006) J Appl Microbiol 100:846–853

    Article  CAS  Google Scholar 

  37. Kohmoto T, Kukui F, Takaku H, Mitsuoka T (1991) Agric Biol Chem 55:2157–2159

    CAS  Google Scholar 

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Correspondence to Michael G. Gänzle.

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Dlusskaya, E., Jänsch, A., Schwab, C. et al. Microbial and chemical analysis of a kvass fermentation. Eur Food Res Technol 227, 261–266 (2008). https://doi.org/10.1007/s00217-007-0719-4

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  • DOI: https://doi.org/10.1007/s00217-007-0719-4

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