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Study on extraction efficiency of natural antioxidant in coffee by capillary electrophoresis with amperometric detection

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Abstract

Capillary electrophoresis with amperometric detection (CE-AD) has been used for the determination of catechin, rutin, ferulic acid, o-dihydroxybenzene, chlorogenic acid, caffeic acid, gallic acid, and protocatechuic acid in coffee. Comparison of extraction efficiency of antioxidants using different polar solvents was also conducted by this method. Effects of several important factors including the acidity and concentration of running buffer, separation voltage, and working electrode potential, were evaluated to obtain the optimum analysis conditions. The working electrode was a 300 um carbon disc electrode at an applied potential of +0.95 V (vs. saturated calomel reference electrode). Under the optimum conditions, the analytes can be well separated within 26 min in a 75 cm length fused-silica capillary (i.d. 2.5 × 10−5 m). The current response was linear over three orders of magnitude with detection limits (S/N = 3) ranging from 6.0 × 10−8 to 3.6 × 10−7 g/mL for all analytes.

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Acknowledgments

The authors are grateful for the financial support provided by the National Science Foundation of China (grant No. 06DZ05125).

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, Shanghai, 200062, China

    Qingcui Chu, Miao Lin, Xueqin Yu & Jiannong Ye

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  1. Qingcui Chu
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  2. Miao Lin
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  3. Xueqin Yu
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  4. Jiannong Ye
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Correspondence to Jiannong Ye.

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Chu, Q., Lin, M., Yu, X. et al. Study on extraction efficiency of natural antioxidant in coffee by capillary electrophoresis with amperometric detection. Eur Food Res Technol 226, 1373–1378 (2008). https://doi.org/10.1007/s00217-007-0667-z

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  • DOI: https://doi.org/10.1007/s00217-007-0667-z

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