Abstract
The effect of pan-frying on the formation of cholesterol oxidation products (COPs) in different processed meat samples (beef patties, braised meat, and fillets of pork) was studied. Samples were pan-fried with or without addition of oil. Different unsaturated oils (olive oil, corn oil or partly hydrogenated plant oil) were used throughout the study. After extraction, seven toxicologically relevant COPs were analyzed using LC–MS. Prior to heat processing up to 6.7 mg COPs/kg extracted fat could be detected in the raw material. Neither the cholestanetriol nor 25-hydroxycholesterol, which are the most cytotoxic COPs in vitro, were detectable in any sample. Differences in the COPs contents were observed between beef (up to 16.5 mg/kg extracted fat) and pork (up to 22.2 mg/kg extracted fat) samples. In prepared samples higher COPs content was noted compared with raw samples. Generally, a certain order of COPs increase dependent on the plant oil used could be recognized: corn oil < partially hydrogenated plant oil < olive oil. It appears that short heating time, mild heating conditions, and the use of fresh and shortly stored raw materials keep COPs levels low.
References
Leonarduzzi G, Sottero B, Poli G (2002) J Nutr Biochem 13:700–710
Russell DW (2000) Biochim Biophys Acta 1529:126–135
Smith LL (1987) Chem Phys Lipids 44:87–125
Bösinger S, Luf W, Brandl E (1993) Int Dairy J 3:1–33
Bodin K, Diczfalusy U (2002) Eur J Lipid Sci Technol 104:435–439
Paniangvait P, King AJ, Jones AD, German BG (1995) J Food Sci 60:1159–1174
Al-Saghir S, Thurner K, Wagner K-H, Frisch G, Luf W, Razzazi-Fazeli E, Elmadfa I (2004) J Agric Food Chem 52:5290–5296
Folch J, Lees M, Sloane-Stanley GM (1957) J Biol Chem 226:497–508
Lai SM, Gray JI, Zabik ME (1995) J Agric Food Chem 43:1122–1126
Razzazi-Fazeli E, Kleineisen S, Luf W (2000) J Chromatogr A 896:321–334
Larkeson B, Dutta PC, Hansson I (2000) J Am Oil Chem Soc 77:675–680
Pie JE, Spahis K, Seillan C (1991) J Agric Food Chem 39:250–254
Hwang KT, Maerker G (1993) J Am Oil Chem Soc 70:371–375
Wasilchuk BA, Le Quesne PW, Vouros P (1992) Anal Chem 64:1077–1087
Savage GP, Dutta PC, Rodriguez-Estrada MT (2002) Asia Pac J Clin Nutr 11:72–78
Smith LL (1981) Initial events of autoxidation. In cholesterol autoxidation, Plenum Press, New York, pp 125–199
Hazell T (1982) J Sci Food Agric 33:1049–1056
Leonhardt M, Wenk C (1997) J Food Compos Anal 10:218–224
Saghir S, Wagner K-H, Elmadfa I (2005) Meat Sci 71:440–445
Li N, Ohshima T, Shozen K, Ushio H, Koizumi C (1994) J Am Oil Chem Soc 71:623–627
Saghir S, Wagner K-H, Elmadfa I (in publication) .
Peng SK, Sevanian A, Morin RJ (1992) Cytotoxicity of cholesterol oxides. In: Peng SH, Morin RJ (eds) Biological Effects of Cholesterol Oxides, CRC Press, Boca Raton, pp 147–166
Acknowledgements
We kindly want to thank AMC International for supplying the Multi-Cooking System. We are also grateful to the International Foundation for the Promotion of Nutrition Research and Nutrition Education (ISFE) for funding this study, No. 275 under their research program.
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Thurner, K., Razzazi-Fazeli, E., Wagner, KH. et al. Determination of cholesterol oxidation products in raw and processed beef and pork preparations. Eur Food Res Technol 224, 797–800 (2007). https://doi.org/10.1007/s00217-006-0372-3
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DOI: https://doi.org/10.1007/s00217-006-0372-3