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Vitamin D and provitamin D in fish

Determination by HPLC with electrochemical detection

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Abstract

A highly-sensitive high-performance liquid chromatographic method for measuring vitamin D3 and provitamin D3 in the edible part of fish was developed. The sample preparation procedure consisted of cold saponification and liquid/liquid extraction of the unsaponifiable matter using a disposable kieselguhr cartridge. For the purification of the sample extracts a semipreparative stage using normal-phase HPLC with UV-detection was applied. A vitamin D and a provitamin D fraction was collected and subsequently quantified by analytical reversed-phase HPLC with electrochemical detection. Internal standardization was used for quantitation. The accuracy of this method was evaluated by recovery experiments. The investigated fish included most of the important fish species consumed in Germany.

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References

  1. Combs GF (1998) In: Combs GF (ed) The vitamins, fundamental aspects in nutrition and health, 2nd edn. Academic Press, San Diego, California, pp 155–187

    Google Scholar 

  2. Trang HM., Cole DE, Rubin LA, Pierratos A, Siu S, Vieth R (1998) Am J Clin Nutr 68:854–858

    CAS  Google Scholar 

  3. Opinion of the Scientific Committee on Food on the Tolerable Upper Intake Level of Vitamin D expressed on 4.12.2002. http://europa.eu.int/comm/food/fs/sc/scf/out157_en.pdf

  4. Barthel HR, Scharla SH (2003) Dtsch Med Wochenschr 128:440–446

    Article  CAS  Google Scholar 

  5. Mehta RG, Mehta RR (2002) J of Nutr Biochem 13:252–264

    Article  CAS  Google Scholar 

  6. Brown AJ, Dusso A, Slatopolsky E (1999) Am J Physiol 277:F157–F175

    CAS  Google Scholar 

  7. Holick MF (2003) J Cell Biochem 88:296–307

    Article  CAS  Google Scholar 

  8. D-A-CH (2000): Referenzwerte für die Nährstoffzufuhr, 1. Auflage Umschau/Braus Verlag, Frankfurt a. Main

    Google Scholar 

  9. Zittermann A (2003) Br J Nutr 89:552–572

    Article  CAS  Google Scholar 

  10. Rao DS, Raghuramulu N (1997) J Nutr Sci Vitaminol 43:425–433

    CAS  Google Scholar 

  11. Rao DS, Raghuramulu N (1999) J Nutr Sci Vitaminol 45:1–8

    CAS  Google Scholar 

  12. Mattila P, Piironen V, Haapala R, Hirvi T, Uusi-Rauva E (1997) J Agric Food Chem 45:3891–3896

    Article  CAS  Google Scholar 

  13. Eitenmiller RR, Landen WO (1998) Vitamin analysis for the health and food sciences, CRC press, pp 77–107

  14. Jones G, Trafford D, Makin H, Hollis BW (1992) In: De Leenheer AP, Lambert WE, Nelis HJ (eds) Modern chromatographic analysis of vitamins, 2nd edn. Marcel Dekker, Inc., pp 73–151

  15. Parrish DB (1979) CRC Critical Rev Food Sci Nutr 12:29–57

    Article  CAS  Google Scholar 

  16. Untersuchung von Lebensmitteln: Bestimmung von Vitamin D (Cholecalciferol (D3) und Ergocalciferol (D2)) in Lebensmitteln mittels HPLC. L 00.00-61, Juli 2001, in: Amtliche Sammlung von Untersuchungsverfahren nach §35 LMBG, Bundesgesundheitsamt (ed), Beuth, Berlin, Köln, (according to DIN EN 12821)

  17. Hasegawa H (1992) J Chromatogr 605:215–220

    Article  CAS  Google Scholar 

  18. Masuda S, Okano T, Kamao M, Kanedai Y, Kobayashi T (1997) J Pharm Biomed Anal 15:1497–1502

    Article  CAS  Google Scholar 

  19. Zamarreno MMD, Pérez AS, Iglesias MCM, Méndez JH (1993) Anal Lett 26:2565–2573

    Google Scholar 

  20. Zamarreno MMD, Pérez AS, Pérez CG, Méndez JH (1992) J Chromatogr. 623:69–74

    Article  Google Scholar 

  21. ESA Application Note 70–4935, www.esainc.com

  22. Hart JP, Norman MD, Lacey CJ (1992) Analyst 117:1441–1445

    Article  CAS  Google Scholar 

  23. Porteous CE, Coldwell RD, Trafford DJH, Makin HLJ (1987) J steroid Biochem 28:785-801

    Article  Google Scholar 

  24. Oehlenschläger J (1986) Inf Fischw 33:188–190

    Google Scholar 

  25. Schilling M, Armbruster FP, Schmidt-Gayk H (1987) Clin Chem 33:187

    CAS  Google Scholar 

  26. Scharla S, Schmidt-Gayk H, Reichel H, Mayer E (1984) Clin Chim Acta 142:323–338

    Article  Google Scholar 

  27. Proszynska K, Lukaszkiewicz J, Jarocewicz N, Lorenc RS (1985) Clin Chim Acta 153:85–92

    Article  CAS  Google Scholar 

  28. Bourgois CF, Ciba N (1988) J Assoc Off Anal Chem 71:12–15

    Google Scholar 

  29. Brinkmann E, Mehlitz I, Oei HB, Tiebach R, Baltes W (1994) Z Lebensm Unters Forsch 199:206–209

    Article  CAS  Google Scholar 

  30. Bilic N, Sieber R (1988) Fresenius Z Anal Chem 330:143–145

    Article  CAS  Google Scholar 

  31. Souci SW, Fachmann W, Kraut H (2000) Die Zusammensetzung der Lebensmittel, Nährwert-Tabellen, 6. Aufl., Medpharm Scientific Publishers, Stuttgart

    Google Scholar 

  32. Bundeslebensmittelschlüssel, http://www.daskochrezept.de/ernaehrung

  33. Mattila P, Piironen V, Uusi-Rauva E, Koivistoinen P (1995) J Food Comp Anal 8:232–243

    Article  CAS  Google Scholar 

  34. Dias MG, Sánchez MV, Bártolo H, Oliveira L (2003) Electron J Environ Agric Food Chem 2

  35. Lall SP, Parazo MP (1995) In: Ruiter A (ed) Fish and fishery products. CAB International, New York, 157–186

    Google Scholar 

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Authors and Affiliations

  1. Federal Research Centre for Nutrition and Food, Department of Fish Quality, Palmaille 9, 22767, Hamburg, Germany

    Ute Ostermeyer & Thomas Schmidt

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  1. Ute Ostermeyer
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  2. Thomas Schmidt
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Correspondence to Ute Ostermeyer.

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Ostermeyer, U., Schmidt, T. Vitamin D and provitamin D in fish. Eur Food Res Technol 222, 403–413 (2006). https://doi.org/10.1007/s00217-005-0086-y

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  • DOI: https://doi.org/10.1007/s00217-005-0086-y

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