Abstract.
Stereoisomers of 8-S-cysteinyl-p-menthan-3-one synthesized from (R)- and (S)- pulegone by Michael addition of cysteine were characterized by means of GC, GC-MS, LC-MS, and 1H-NMR. Conjugates were treated with three sources of cysteine-S-conjugate β-lyase: (i) a crude enzyme extract prepared from Eubacterium limosum, (ii) tryptophanase from E. coli and (iii) yeast cells. The result was liberation of 8-mercapto-p-menthan-3-one, a powerful flavoring substance exhibiting a cassis-type odor note. The enzyme-catalyzed cleavage of the thio-ether bond proceeded with low enantioselectivity. Discrimination of diastereoisomers was more pronounced with a clear preference of the cis-configured substrates.
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Wakabayashi, H., Wakabayashi, M., Eisenreich, W. et al. Stereoselectivity of the β-lyase-catalyzed cleavage of S-cysteine conjugates of pulegone. Eur Food Res Technol 215, 287–292 (2002). https://doi.org/10.1007/s00217-002-0576-0
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DOI: https://doi.org/10.1007/s00217-002-0576-0