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Recent advances in bioprobes and biolabels based on cyanine dyes

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Abstract

As a functional dye, cyanine dye promotes the widespread application of bioprobes in the fields of medicine, genetics and environment, owing to its advantages of good photophysical properties, excellent biocompatibility and low toxicity to biological systems. Nowadays, it is mainly used in the fields of life sciences such as fluorescent labeling of biological macromolecules, disease diagnosis, immunoassay and DNA detection, all of which lie at the core of this review. First, we briefly introduced the characteristics and principles of the cyanine dye bioprobe. Afterward, we paid attention to the recent progress of cyanine dye bioprobes widely used in the 10 years from 2010 to 2020. The application of cyanine dyes as bioprobes with different identification elements, including enzymes, organelles, immunity and DNAs, was mainly summarized. Finally, this review gave an outlook on the future development trend of cyanine dye bioprobes. This facilitates the construction of a new type of multifunctional fluorescent probe and promotes its clinical application.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant Number 21807034) and Natural Science Foundation of Hebei Province (No. B2020209079).

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  1. College of Chemical Engineering, Hebei and Tangshan Key Laboratory of Medical-Industrial Integration Precision Medicine, North China University of Science and Technology, 063210, Tangshan, China

    Xiaoying Ma, Lei Shi, Buyue Zhang, Lu Liu, Yao Fu & Xiufeng Zhang

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  1. Xiaoying Ma
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Ma, X., Shi, L., Zhang, B. et al. Recent advances in bioprobes and biolabels based on cyanine dyes. Anal Bioanal Chem 414, 4551–4573 (2022). https://doi.org/10.1007/s00216-022-03995-8

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