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Detection of aflatoxin B1 with a new label-free fluorescence aptasensor based on PVP-coated single-walled carbon nanohorns and SYBR Gold

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Abstract

This paper describes a novel fluorescence label-free aptasensor to detect aflatoxin B1 (AFB1) by utilizing SYBR Gold, aptamer, and single-walled carbon nanohorns (SWCNHs). In the presence of AFB1, the conformation of AFB1-specific aptamer went through and the spatial structure of this specific aptamer was transformed accordingly. Due to the resistance of the transformed aptamer when adsorbed on the surface of SWCNHs, the protection of the fluorescence of SYBR Gold was accomplished. Consequently, concentrations of AFB1 showed a strong association with fluorescence intensity. The detection limit (LOD) of AFB1 was 1.89 ng/mL, while the linear range was 5–200 ng/mL and fluorescence intensity satisfactorily correlated (R2 = 0.9919) with the logarithm of AFB1 concentration.

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Funding

This work was supported by the Department of Sciences and Technology of Jilin Province(20200403044SF), the State Key Research and Development Plan (2018YFD0400104), the Higher Education Discipline Innovation Project (111 Project, D18012), the Department of Education of Jilin Province (JJKH20200516KJ), and the Training Program of Ronghe College, Yanbian University (012021081).

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Authors and Affiliations

  1. Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Yanbian University, Yanji, 133002, China

    Xin Qi, Lei Lv, Jung Joon Lee, Chengbi Cui & Zhijun Guo

  2. College of Pharmacy, Yanbian University, Yanji, 133002, China

    Xin Qi & Chengbi Cui

  3. College of Agriculture, Yanbian University, Yanji, 133002, China

    Lei Lv, Mengyu Niu & Zhijun Guo

  4. Technology Center of Harbin Customs, Harbin, 150008, China

    Dongxu Wei

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  1. Xin Qi
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Qi, X., Lv, L., Wei, D. et al. Detection of aflatoxin B1 with a new label-free fluorescence aptasensor based on PVP-coated single-walled carbon nanohorns and SYBR Gold. Anal Bioanal Chem 414, 3087–3094 (2022). https://doi.org/10.1007/s00216-022-03938-3

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