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Label-free magnetic nanoparticles-based electrochemical immunosensor for atrazine detection

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Abstract

This work presents the realization of a label-free electrochemical immunosensor for the quick, cheap, and straightforward determination of atrazine. This biodevice is based on developing a technological platform where a gold screen printed electrode (Au-SPE) surface was modified by the electrodeposition of a highly porous gold layer. As an internal probe redox, a Prussian Blue thin layer (PB) was then electrosynthetized onto the modified Au-SPE. Atrazine antibody (Ab-ATZ) was immobilized using G protein-functionalized magnetic nanoparticles (MNPs@protG) to ensure the correct orientation of the antibody to enhance the immunoaffinity. Under optimum experimental conditions, the electrochemical characterization of the developed immunosensor displays a linearity range towards atrazine within 0.05–1.5 ng/mL, a LOD of 0.011 ng/mL good reproducibility and stability. The immunosensor was tested in the analysis of spiked drinking water samples with a mean recovery ranging from 95.7 to 108.4%. The overall good analytical performances of this immunodevice suggest its application for the screening and monitoring of atrazine in real matrices.

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Authors and Affiliations

  1. Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy

    Rosaceleste Zumpano, Manuela Manghisi, Francesca Polli, Cristine D’Agostino & Franco Mazzei

  2. Dipartimento Innovazioni Tecnologiche E Sicurezza Degli Impiantiprodotti e insediamenti antropici, Istituto Nazionale Per L’Assicurazione Contri Gli Infortuni Sul Lavoro (INAIL), 00143, Rome, Italy

    Federica Ietto

  3. Department of Environmental Biology, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy

    Gabriele Favero

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  1. Rosaceleste Zumpano
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Zumpano, R., Manghisi, M., Polli, F. et al. Label-free magnetic nanoparticles-based electrochemical immunosensor for atrazine detection. Anal Bioanal Chem 414, 2055–2064 (2022). https://doi.org/10.1007/s00216-021-03838-y

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