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A novel ESIPT fluorescent probe derived from 3-hydroxyphthalimide for hydrazine detection in aqueous solution and living cells

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Abstract

Hydrazine is a highly toxic and flammable liquid that can damage human liver, kidney, and central nervous system. Therefore, it is valuable to seek a quick and sensitive method for hydrazine detection in environmental and biological science. Herein, a new fluorescent probe derived from 3-hydroxyphthalimide was synthesized. This probe can rapidly and selectively detect hydrazine with a low detection limit of 4.3 × 10−7 M. The recognition principle is based on hydrazine-induced acetyl deprotection and excited-state intramolecular proton transfer (ESIPT) process. Moreover, test paper and fluorescence image experiments showed that this probe had potential to monitor hydrazine in the environment and living cells.

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Funding

The work was supported by the Hubei Province Education Ministry Foundation of China (D20112507), the National Natural Science Foundation of China (20872042), the Major Program of Technical Innovation Special Project in Hubei Province (2019ABA112), and the Central Government Guided Local Science and Technology Development Project of Hubei Province (2017ZYYD008).

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Authors and Affiliations

  1. Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, Hubei, China

    Shengli Hu, Junyu Wang, Meiling Luo & Zhou Wu

  2. School of Life Science, Hubei Normal University, Huangshi, 435002, Hubei, China

    Yaoyao Hou & Xinpeng Chen

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  1. Shengli Hu
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  2. Junyu Wang
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  3. Meiling Luo
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  4. Zhou Wu
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  5. Yaoyao Hou
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  6. Xinpeng Chen
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Correspondence to Shengli Hu or Xinpeng Chen.

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Hu, S., Wang, J., Luo, M. et al. A novel ESIPT fluorescent probe derived from 3-hydroxyphthalimide for hydrazine detection in aqueous solution and living cells. Anal Bioanal Chem 413, 5463–5468 (2021). https://doi.org/10.1007/s00216-021-03530-1

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  • DOI: https://doi.org/10.1007/s00216-021-03530-1

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