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Ultrasensitive detection of trypsin in serum via nanochannel device

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Abstract

A highly sensitive trypsin sensing system in serum was developed by using an anodic alumina oxide (AAO)-based, trypsin substrate-decorated hybrid ion permeation membrane. Owing to the trypsin-triggered peptide hydrolyzation reaction, the surface electrical feature of the peptide-decorated hybrid ion membrane changed. The electric double layer effect reduces the effective ion current diameter in the AAO nano unit, so that the ion current rectification ratio will be enhanced, realizing the quantitative detection of trypsin. The lowest detection concentration can be achieved as low as 0.1 pM. This method is no need for sample pre-preparation, easy to operate, highly sensitive, and also applicable to other enzyme evaluation systems by changing corresponding substrates. This study provides a new idea for selective measurements of proteases in complex biological samples.

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Funding

This work was supported by the National Natural Science Foundation of China (21873039), the Project was Supported by the State Key Laboratory of Applied Optics (sklssm2021016), and Interdisciplinary Integration Innovation Project of Jilin University  (JLUXKJC2020106).

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Authors and Affiliations

  1. State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, Jilin, People’s Republic of China

    Shulin Guan, Jing Yue, Weiqing Xu & Shuping Xu

  2. Institute of Frontier Medical Science, Jilin University, Changchun, Jilin, 130021, People’s Republic of China

    Weihan Sun & Chongyang Liang

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  1. Shulin Guan
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  2. Jing Yue
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  3. Weihan Sun
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  4. Weiqing Xu
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  5. Chongyang Liang
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Corresponding authors

Correspondence to Chongyang Liang or Shuping Xu.

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For ethical reasons, we need to stress that the serum used in the experiment was bovine serum. We did not use human serum in the study.

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Guan, S., Yue, J., Sun, W. et al. Ultrasensitive detection of trypsin in serum via nanochannel device. Anal Bioanal Chem 413, 4939–4945 (2021). https://doi.org/10.1007/s00216-021-03491-5

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  • DOI: https://doi.org/10.1007/s00216-021-03491-5

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