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Development of ELISA and chemiluminescence enzyme immunoassay for quantification of histamine in drug products and food samples

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Abstract

Histamine (HA) is a biogenic amine associated with allergies and food poisoning. It is an important indicator of food freshness and quality. In recent years, a series of medical negligence cases have been reported to be related to the intravenous injection of antibiotics produced via fermentation with fish peptone due to HA contamination. To detect HA efficiently, mouse monoclonal antibody was developed. An enzyme-linked immunosorbent assay (ELISA) and a chemiluminescence enzyme immunoassay (CLEIA) were developed and compared with conventional HPLC analysis. Both immunoassays showed low cross-reactivity, low 50% inhibitive concentration (IC50; 1.2 μg/mL and 1.1 μg/mL), low limits of detection (LODs, IC10; 89.0 ng/mL and 73.4 ng/mL), and appreciable recoveries in spiked foods and drugs (from 73.4 to 131.0% and from 77.0 to 119.0%, espectively), demonstrating that the developed methods are sensitive, specific, fast, and reliable for HA detection in complicated real samples.

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Funding

This work is financially supported by the National Natural Science Foundation of China (Nos. 31572556 and 31873006); the Key Program for International S&T Cooperation Project of Shaanxi Province (No. 2017KW-ZD-10); the Ministry of Education and Science of the Russian Federation (No. RFMEFI60417X0198); the FCT I.P, the Ministério da Ciência, Tecnologia e Ensino Superior (MCTES); and the European Regional Development Fund (ERDF) (No. POCI-01-0145-FEDER-007569).

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Author notes

  1. Long Xu and Jiping Zhou contributed equally to this work.

Authors and Affiliations

  1. College of Biological Science and Engineering, Shaanxi University of Technology, East on the 1st Ring Road, Hanzhong, 723000, Shaanxi, China

    Long Xu, Jiping Zhou & Xiaoying Zhang

  2. Department of Biology, Centre of Molecular and Environmental Biology, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Long Xu, Alberto C. P. Dias & Xiaoying Zhang

  3. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China

    Jiping Zhou

  4. Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia

    Sergei Eremin

  5. College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, 712100, Shaanxi, China

    Xiaoying Zhang

  6. Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada

    Xiaoying Zhang

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  1. Long Xu
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Correspondence to Xiaoying Zhang.

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Ethics statement

All procedures performed in this study involving mice were in accordance with the experimental animal regulation of China and according to the measures for the administration of experimental animal in Shaanxi Province concerning protection of animals used for scientific purposes. All experimental animal protocols were reviewed and approved by the Ethics Committee of Shaanxi University of Technology for the use of laboratory animals (No. 2019-010).

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Xu, L., Zhou, J., Eremin, S. et al. Development of ELISA and chemiluminescence enzyme immunoassay for quantification of histamine in drug products and food samples. Anal Bioanal Chem 412, 4739–4747 (2020). https://doi.org/10.1007/s00216-020-02730-5

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  • DOI: https://doi.org/10.1007/s00216-020-02730-5

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