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Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

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Abstract

In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.

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Acknowledgments

Financial support was provided by Grant Agency of Czech Republic (GACR 17-12774S) and project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Program II. LPV was supported by project 6SA17676 that received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie and which is co-financed by the South Moravian Region under the grant agreement No. 665860.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic

    Michaela Tvrdonova, Viktor Kanicky & Tomas Vaculovic

  2. CEITEC, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic

    Michaela Tvrdonova & Viktor Kanicky

  3. Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic

    Marcela Vlcnovska, Lucie Pompeiano Vanickova, Vojtech Adam & Marketa Vaculovicova

  4. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic

    Lucie Pompeiano Vanickova, Vojtech Adam, Marketa Vaculovicova & Tomas Vaculovic

  5. Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Straße 11, 12489, Berlin, Germany

    Lena Ascher & Norbert Jakubowski

Authors
  1. Michaela Tvrdonova
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  2. Marcela Vlcnovska
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  3. Lucie Pompeiano Vanickova
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  4. Viktor Kanicky
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  5. Vojtech Adam
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  6. Lena Ascher
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  7. Norbert Jakubowski
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  8. Marketa Vaculovicova
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  9. Tomas Vaculovic
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Correspondence to Tomas Vaculovic.

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Published in the topical collection Elemental and Molecular Imaging by LA-ICP-MS with guest editor Beatriz Fernández García.

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Tvrdonova, M., Vlcnovska, M., Vanickova, L.P. et al. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Anal Bioanal Chem 411, 559–564 (2019). https://doi.org/10.1007/s00216-018-1300-7

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  • DOI: https://doi.org/10.1007/s00216-018-1300-7

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