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Molecularly imprinted polymers combined with membrane-protected solid-phase extraction to detect triazines in tea samples

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Abstract

Spherical molecularly imprinted polymers (MIPs) were prepared by emulsion polymerization. The isothermal adsorption and selective adsorption indicated that the MIPs obtained exhibit excellent specific recognition for the template (atrazine) and its analogues. The MIPs were encapsulated in a polypropylene microporous membrane to fabricate MIP adsorption packages for the direct extraction of triazines in uncentrifuged and unfiltered tea extracts. The extraction conditions affecting the extraction efficiency, including the type and volume of extraction solvent, the number of MIP adsorption packages, the surface area of the MIP adsorption packages, the mass of MIPs in the MIP adsorption packages, the extraction time, the eluting solvent, and the eluting volume, were optimized. Under the optimal extraction and high-performance liquid chromatography–tandem mass spectrometry conditions, the method exhibited excellent linearity in the range from 0.5 to 250 ng g-1, with R2 ≥ 0.9992. The detection limit of the method was 0.09–0.18 ng g-1. The intraday and interday relative standard deviations ranged from 3.1% to 7.5% and from 3.1% to 7.9%, respectively. The method was successfully used to detect triazines in five tea samples. At a spiking concentration of 2 ng g-1, satisfactory recoveries ranging from (81 ± 3)% to (104 ± 7)% were obtained. The membrane-protected solid-phase extraction method based on molecularly imprinted material is expected to be widely used to enrich triazines in complex samples.

Schematic illustration of the MIPs combined with membrane-protected solid-phase extraction of triazines in tea sample

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Acknowledgements

This work was supported by the Open Project of the State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (no. QAK201809) and the Development Program of the Ministry of Science and Technology of Jilin Province, China (grant number 20150204070GX).

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Author notes

  1. Tianyu Zhou and Qi Zhao contributed equally to this work.

Authors and Affiliations

  1. College of Chemistry, Jilin University, Changchun, Jilin, 130012, China

    Tianyu Zhou, Qi Zhao, Li Zhao, Haochi Liu, Bo Wang, Ning Huang & Lan Ding

  2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 202 Haihe Road, Nangang District, Harbin, 150090, Heilongjiang, China

    Jie Ding

  3. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun, 130012, Jilin, China

    Yi Li

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  1. Tianyu Zhou
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  2. Qi Zhao
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  3. Li Zhao
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  8. Lan Ding
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Correspondence to Jie Ding or Lan Ding.

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Zhou, T., Zhao, Q., Zhao, L. et al. Molecularly imprinted polymers combined with membrane-protected solid-phase extraction to detect triazines in tea samples. Anal Bioanal Chem 410, 5173–5181 (2018). https://doi.org/10.1007/s00216-018-1171-y

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  • DOI: https://doi.org/10.1007/s00216-018-1171-y

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