Skip to main content
SpringerLink
Log in

Development of a molecular recognition based approach for multi-residue extraction of estrogenic endocrine disruptors from biological fluids coupled to liquid chromatography-tandem mass spectrometry measurement

  • Research Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Multi-residue methods permitting the high-throughput and affordable simultaneous determination of an extended range of endocrine disrupting chemicals (EDCs) with reduced time and cost of analysis is of prime interest in order to characterize a whole set of bioactive compounds. Such a method based on UHPLC-MS/MS measurement and dedicated to 13 estrogenic EDCs was developed and applied to biological matrices. Two molecular recognition-based strategies, either molecular imprinted polymer (MIP) with phenolic template or estrogen receptors (ERα) immobilized on a sorbent, were assessed in terms of recovery and purification efficiency. Both approaches demonstrated their suitability to measure ultra-trace levels of estrogenic EDCs in aqueous samples. Applicability of the MIP procedure to urine and serum samples has also been demonstrated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Skakkebaek N, Rajpert-De Meyts E, Main KM (2001) Hum Reprod 16:972–978

    Article  CAS  Google Scholar 

  2. Zhou F, Zhang L, Liu A, Shen Y, Yuan J, Yu X, Feng X, Xu Q, Cheng C (2013) J Chromatogr B 938:80–85

    Article  CAS  Google Scholar 

  3. Shen Y, Ren M-L, Feng X, Gao Y-X, Xu Q, Cai Y-L (2014) Eur J Obstet Gynecol Reprod Biol 178:137–137

    Google Scholar 

  4. Tang R, Chen M-J, Ding G-D, Chen X-J, Han X-M, Zhou K, Chen L-M, Xia Y-K, Tian Y, Wang X-R (2013) Environ Pollut 178:115–120

    Article  CAS  Google Scholar 

  5. Casals-Casas C, Desvergne B (2011) Annu Rev Physiol 73:135–162

    Article  CAS  Google Scholar 

  6. Virtanen HE, Adamsson A (2012) Mol Cell Endocr 355:208–220

    Article  CAS  Google Scholar 

  7. Grün F, Blumberg B (2009) Mol Cell Endocr 304:19–29

    Article  Google Scholar 

  8. Ziegler RG, Faupel-Badger JM, Sue LY, Fuhrman BJ, Falk RT, Boyd-Morin J, Henderson MK, Hoover RN, Veenstra TD, Keefer LK, Xu X (2010) J Steroid Biochem Mol Biol 121:538–545

    Article  CAS  Google Scholar 

  9. Diamanti-Kandarakis E, Bourguignon J-P, Giudice LC, Hauser R, Prins GS, Soto AM, Zoeller RT, Gore AC (2009) Endocr Rev 30:293–342

    Article  CAS  Google Scholar 

  10. Aris A (2014) Reprod Toxicol 45:8–13

    Article  CAS  Google Scholar 

  11. Prasain JK, Arabshahi A, Moore RD II, Greendale GA, Wyss JM, Barnes S (2010) J Chromatogr B 878:994–1002

    Article  CAS  Google Scholar 

  12. Ferrara F, Ademollo N, Orrù MA, Silvestroni L, Funari E (2011) Chemosphere 82:1044–1049

    Article  CAS  Google Scholar 

  13. Kushnir MM, Rockwood AL, Bergquist J, Varshavsky M, Roberts WL, Yue B, Bunker AM, Meikle AW (2008) Am J Clin Pathol 129:530–539

    Article  CAS  Google Scholar 

  14. Pichon V, Chapuis-Hugon F (2008) Anal Chim Acta 622:48–61

    Article  CAS  Google Scholar 

  15. Hu Y, Pan J, Zhang K, Lian H, Li G (2013) Trends Anal Chem 43:37–52

    Article  CAS  Google Scholar 

  16. Zhang J-H, Jiang M, Zou L, Shi D, Mei S-R, Zhu Y-X, Shi Y, Dai K, Lu B (2006) Anal Bioanal Chem 385:780–786

    Article  CAS  Google Scholar 

  17. Gadzała-Kopciuch R, Ričanyová J, Buszewski B (2009) J Chromatogr B 877:1177–1184

    Article  Google Scholar 

  18. Chrzanowska AM, Poliwoda A, Wieczorek PP (2015) J Chromatogr A 1392:1–9

    Article  CAS  Google Scholar 

  19. Le Maire A, Bourguet W, Balaguer P (2010) Cell Mol Life Sci 67:1219–1237

    Article  CAS  Google Scholar 

  20. Nguyen HP, Li L, Gatson JW, Maass D, Wigginton JG, Simpkins JW, Schug KA (2011) J Pharma Biomed Anal 54(4):830–837

  21. Lin Y-H, Chen C-Y, Wang G-S (2007) Rapid Commun Mass Spectrom 21:1973–1983

    Article  CAS  Google Scholar 

  22. Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (Text with EEA relevance) (notified under document number C(2002) 3044). Off J Eur Commun 2002:L221, 8

  23. Balaguer P, François F, Comunale F, Fenet H, Boussioux A-M, Pons M, Nicolas J-C, Casellas C (1999) Sci Total Environ 233:47–56

    Article  CAS  Google Scholar 

  24. Li W, Li Y-H, Li AC, Zhou S, Naidong W (2005) J Chromatogr B 825:223–232

    Article  CAS  Google Scholar 

  25. Blonder J, Johann DJ, Veenstra TD, Xiao Z, Emmert-Buck MR, Ziegler RG, Rodriguez-Canales J, Hanson JA, Xu X (2008) Anal Chem 80:8845–8852

    Article  CAS  Google Scholar 

  26. Xu L, Spink DC (2008) Anal Biochem 375:105–114

    Article  CAS  Google Scholar 

  27. Pillon A, Boussioux A-M, Escande A, Aït-Aïssa S, Gomez E, Fenet H, Ruff M, Moras D, Vignon F, Duchesne M-J, Casellas C, Nicolas J-C, Balaguer P (2005) Environ Health Perspect 113(3):278–284

    Article  CAS  Google Scholar 

  28. Liu Z-H, Kanjo Y, Mizutani S (2010) Water Res 44:567–577

    Article  CAS  Google Scholar 

  29. Molina-Molina J-M, Amaya E, Grimaldi M, Sáenz J-M, Real M, Fernández MF, Balaguer P, Olea N (2013) Toxicol Appl Pharmacol 272:127–136

    Article  CAS  Google Scholar 

  30. Chandsawangbhuwana C, Baker ME (2014) Steroids 80:37–43

    Article  CAS  Google Scholar 

  31. Delfosse V, Grimaldi M, Cavaillès V, Balaguer P, Bourguet W (2014) Environ Health Perspect 122(12):1306–1313

    CAS  Google Scholar 

  32. Delfosse V, Grimaldi M, Pons J-L, Boulahtouf A, Le Maire A, Cavailles V, Labesse G, Bourguet W, Balaguer P (2012) Proc Natl Acad Sci U S A 109:14930–14935

    Article  CAS  Google Scholar 

  33. Adlercreutz H, Yamada T, Wähälä K, Watanabe S (1999) Am J Obstet Gynecol 180(3):737–743

    Article  CAS  Google Scholar 

  34. Kuijper E, Ket JCF, Caanen MR, Lambalk CB (2013) Reprod Biomed Online 27:33–63

    Article  CAS  Google Scholar 

  35. Pirard C, Sagot C, Deville M, Dubois N, Charlier C (2012) Environ Int 48:78–83

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors want to express their special thanks to all the volunteers who have permitted the collection of biological samples exploited in this work. They also thank the Centre Hospitalier Universitaire de Toulouse as the promoter of the corresponding clinical protocol.

Conflict of Interest

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. LUNAM Université, Oniris, USC 1329 Laboratoire d’Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307, Nantes, France

    Radia Bousoumah, Jean Philippe Antignac, Emmanuelle Bichon, Marie-Line Morvan & Bruno Le Bizec

  2. AgroParisTech, UMR1145 Ingénierie Procédés Aliments, 75005, Paris, France

    Radia Bousoumah & Valérie Camel

  3. INRA, 44307, Nantes, France

    Jean Philippe Antignac & Valérie Camel

  4. INRA, 75005, Paris, France

    Jean Philippe Antignac & Valérie Camel

  5. INSERM Institut National de la santé et de la Recherche Médicale, U1194 IRCM, Institut régional du Cancer Montpellier, Université Montpellier, 34298, Montpellier, France

    Marina Grimaldi & Patrick Balaguer

  6. Département Sciences de l’Environnement et Santé Publique, Faculté de Pharmacie, UMR Hydrosciences, Université Montpellier 1, 34298, Montpellier, France

    Frederique Courant

Authors
  1. Radia Bousoumah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jean Philippe Antignac
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Valérie Camel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marina Grimaldi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Patrick Balaguer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Frederique Courant
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Emmanuelle Bichon
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Marie-Line Morvan
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Bruno Le Bizec
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jean Philippe Antignac.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PDF 71 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bousoumah, R., Antignac, J.P., Camel, V. et al. Development of a molecular recognition based approach for multi-residue extraction of estrogenic endocrine disruptors from biological fluids coupled to liquid chromatography-tandem mass spectrometry measurement. Anal Bioanal Chem 407, 8713–8723 (2015). https://doi.org/10.1007/s00216-015-9024-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-015-9024-4

Keywords

Search

Navigation