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Monitoring metabolites from Schizophyllum commune interacting with Hypholoma fasciculare combining LESA–HR mass spectrometry and Raman microscopy

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Abstract

Microbial competition for territory and resources is inevitable in habitats with overlap between niches of different species or strains. In fungi, competition is brought about by antagonistic mycelial interactions which alter mycelial morphology, metabolic processes, secondary metabolite release, and extracellular enzyme patterns. Until now, we were not able study in vivo chemical interactions of different colonies growing on the same plate. In this report, we developed a fast and least invasive approach to identify, quantify, and visualize co culture-induced metabolites and their location of release within Schizophyllum commune. The pigments indigo, indirubin, and isatin were used as examples to show secondary metabolite production in the interaction zone with Hypholoma fasciculare. Using a combinatory approach of Raman spectroscopy imaging, liquid extraction surface analysis (LESA), and high-resolution mass spectrometry, we identified, quantified, and visualized the presence of indigo and indirubin in the interaction zone. This approach allows the investigation of metabolite patterns between wood degrading species in competition to gain insight in community interactions, but could also be applied to other microorganisms. This method advances analysis of living, still developing colonies and are in part not destructive as Raman spectroscopy imaging is implemented.

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Acknowledgments

We gratefully acknowledge financial support from the Deutsche Forschungsgemeinschaft (Jena School of Microbial Communication—MikroInter). Thanks to Imam Hardiman for conceiving the idea of the co-cultures and Elke-Martina Jung for help with Fig. 1. We thank Dr. Matthias Gube for providing strains and Petra Mitscherlich for general technical help.

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Authors and Affiliations

  1. Department of Microbial Communication, Institute of Microbiology, Friedrich Schiller University, Neugasse 25, 07743, Jena, Germany

    Riya C. Menezes, Katrin Krause & Erika Kothe

  2. Leibniz Institute of Photonic Technology e.V., Albert Einstein Straße 9, 07745, Jena, Germany

    Riya C. Menezes, Christian Matthäus & Jürgen Popp

  3. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University, Helmholtzweg 4, 07743, Jena, Germany

    Christian Matthäus & Jürgen Popp

  4. Research Group Mass Spectrometry, Max Planck Institute for Chemical Ecology, Hans Knöll Straße 8, 07745, Jena, Germany

    Marco Kai & Aleš Svatoš

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  1. Riya C. Menezes
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  2. Marco Kai
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Correspondence to Aleš Svatoš.

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Published in the topical collection Mass Spectrometry Imaging with guest editors Andreas Römpp and Uwe Karst.

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Menezes, R.C., Kai, M., Krause, K. et al. Monitoring metabolites from Schizophyllum commune interacting with Hypholoma fasciculare combining LESA–HR mass spectrometry and Raman microscopy. Anal Bioanal Chem 407, 2273–2282 (2015). https://doi.org/10.1007/s00216-014-8383-6

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  • DOI: https://doi.org/10.1007/s00216-014-8383-6

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